A5015063334- Analytical Chemistry and Chromatography
- Pharmacogenetics and Drug Metabolism
- Protein purification and stability
- Polymer Surface Interaction Studies
- Drug Transport and Resistance Mechanisms
- Advanced Polymer Synthesis and Characterization
- Amino Acid Enzymes and Metabolism
- Extracellular vesicles in disease
- Biochemical Analysis and Sensing Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Olfactory and Sensory Function Studies
- Glycosylation and Glycoproteins Research
- Cancer therapeutics and mechanisms
- Hydrogels: synthesis, properties, applications
- Epigenetics and DNA Methylation
- Ginkgo biloba and Cashew Applications
- Lymphoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Innovative Microfluidic and Catalytic Techniques Innovation
- Molecular Sensors and Ion Detection
- Diabetes Treatment and Management
- Supramolecular Self-Assembly in Materials
- Acute Lymphoblastic Leukemia research
- Neutropenia and Cancer Infections
Daiichi-Sankyo (Japan)
2019-2023
Keio University
2014-2022
Poly(N-isopropylacrylamide) (PNIPAAm)-based temperature-responsive fluorescence polymer probes were developed using radical polymerization, with 3-mercaptopropionic acid as the chain-transfer agent, followed by activation of terminal carboxyl groups N-hydroxysuccinimide and reaction 5-aminofluorescein (FL). The lower critical solution temperatures (LCSTs) resulting fluorescent differed depending on copolymer composition, had a sharp phase-transition (hydrophilic/hydrophobic) boundary at...
L-type amino acid transporter 1 (LAT1) is a that more highly expressed in cancer cells compared with normal cells. In the present study, liposomes, composed of egg phosphatidylcholine (EPC) and dioleoyl phosphatidylethanolamine, were modified LAT1-targeting thermoresponsive polymer, l-tyrosine-conjugated poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) (P(NIPAAm-co-DMAAm)). The cellular uptake prepared liposomes was evaluated using HeLa as cell model. At temperatures above polymer's...
The existing methods for exosome isolation, such as ultracentrifugation, size exclusion, and affinity separation, suffer from some limitations. Herein, we aimed to develop temperature-modulated exosome-capturing materials using thermoresponsive polymers peptides with exosomes. Poly(2-hydroxyethyl methacrylate-co-propargyl acrylate)-b-poly(N-isopropylacrylamide) (P(HEMA-co-PgA)-b-PNIPAAm) was grafted on silica beads via a two-step process of activator regenerated by electron transfer atom...
Abstract High-performance liquid chromatography (HPLC) is the most common analytical method practiced in various fields and used for analysis of almost all drug compounds pharmaceutical industries. During development, an evaluation potential interaction with cytochrome P450 (CYP) essential. A “cocktail” approach often development to evaluate effect a candidate on multiple CYP enzymes single experiment. So far, simultaneous substrates, which have greatly different structure physicochemical...
Abstract Valemetostat is an oral, selective inhibitor of enhancer zeste homolog‐2 (EZH2) and EZH1. In a first‐in‐human phase‐1 trial, valemetostat capsules were well tolerated clinically active in patients with relapsed/refractory non‐Hodgkin lymphoma. Subsequently, film‐coated tablet formulation was developed for future clinical trials commercialization. We report outcomes from 2 phase 1 healthy Japanese participants, assessing the safety, tolerability, pharmacokinetics (PK) tablets at...
Abstract Oligonucleotide therapeutics have contributed remarkably to healthcare, being well suited for the treatment of intractable diseases that are difficult approach using conventional drug modalities. However, as common techniques oligonucleotide analysis rely on reversed-phase or ion-exchange liquid chromatography and thus employ toxic organic solvents and/or ion-pairing reagents, better alternatives highly sought after. Poly( N -isopropylacrylamide) (PNIPAAm) is widely used in...
Abstract Valemetostat tosylate (valemetostat) is an oral, potent, dual inhibitor of enhancer zeste homolog (EZH) 2 and EZH1 under investigation for the treatment cancer, including non‐Hodgkin's lymphomas solid tumors. Itraconazole fluconazole are antifungal medications often used as typical inhibitors cytochrome P450 3A (CYP3A [itraconazole fluconazole]) P‐glycoprotein (P‐gp [itraconazole]) in drug–drug interaction studies. a substrate CYP3A P‐gp vitro. This phase I, open‐label,...
In recent years, drug discovery and therapeutics trends have shifted from a focus on small-molecule compounds to biopharmaceuticals, genes, cell therapy, regenerative medicine. Therefore, new approaches technologies must be developed respond these changes in medical care. To achieve this, we applied temperature-responsive separation system purify variety of proteins cells. We chromatography technique based poly(N-isopropylacrylamide) (PNIPAAm)-grafted stationary phase. This method may...
Memantine hydrochloride, an N-methyl-D-aspartate receptor antagonist, is used to treat Alzheimer's disease (AD). A new dry syrup formulation containing memantine hydrochloride has been developed improve medication adherence in AD patients and reduce family caregiver burden. This study was conducted assess the bioequivalence of this tablet. Two single-dose, randomized, open-label, two-period, two-group, crossover studies were a test product [dry syrup, 2%, 1 g (containing 20 mg...