A5015115334- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Selenium in Biological Systems
- Invertebrate Immune Response Mechanisms
- Chromosomal and Genetic Variations
- Genetics, Aging, and Longevity in Model Organisms
- Genomics and Phylogenetic Studies
- Sperm and Testicular Function
- Planarian Biology and Electrostimulation
- Epigenetics and DNA Methylation
- Hippo pathway signaling and YAP/TAZ
- Melanoma and MAPK Pathways
- Ubiquitin and proteasome pathways
- Marine Ecology and Invasive Species
- Cancer-related gene regulation
- Trace Elements in Health
- Cancer-related molecular mechanisms research
- Cancer-related Molecular Pathways
- Heat shock proteins research
- PI3K/AKT/mTOR signaling in cancer
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neuroblastoma Research and Treatments
- Silk-based biomaterials and applications
- PARP inhibition in cancer therapy
Universitat de Barcelona
2013-2025
Institut de Biomedicina de la Universitat de Barcelona
2009-2024
Institut d'Estudis Catalans
2022-2024
Catalan Society of Family and Community Medicine
2022
Institució Catalana de Recerca i Estudis Avançats
2018-2019
Centro de Investigación Biomédica en Red
2009
Universitat de València
2009
Barcelona Biomedical Research Park
2009
Institut Químic de Sarrià
2004
Universitat Ramon Llull
2004
Abstract We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. mobilized pair engineered P elements, p{RS3} and p{RS5}, to collect 3243 lines unambiguously mapped the genome sequence. The contains, on average, an element every 35 kb. demonstrate deletions their end points mapped, with base-pair resolution, was generated isogenic strain, thus affording uniform background screens where...
November 2020 marked 2 y since the launch of Earth BioGenome Project (EBP), which aims to sequence all known eukaryotic species in a 10-y timeframe. Since then, significant progress has been made across aspects EBP roadmap, as outlined 2018 article describing project’s goals, strategies, and challenges (1). The phase ended clock started on reaching EBP’s major milestones. This Special Feature explores many facets EBP, including review progress, description scientific exemplar projects,...
Upon apoptotic stimuli, epithelial cells compensate the gaps left by dead activating proliferation. This has led to proposal that dying signal surrounding living maintain homeostasis. Although nature of these signals is not clear, reactive oxygen species (ROS) could act as a signaling mechanism they can trigger pro-inflammatory responses protect epithelia from environmental insults. Whether ROS emerge and what genetic response triggered pivotal understand regeneration Drosophila imaginal...
We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% the Release 5.1 genome. Using previously reported collection FRT-bearing P-element insertions, we have generated 655 new and verified set 209 deletion-bearing fly stocks. In addition to deletions, demonstrate how P elements may also be used generate custom inversions duplications, particularly useful balancing difficult...
Regeneration and tissue repair allow damaged or lost body parts to be replaced. After injury fragmentation of Drosophila imaginal discs, regeneration leads the development normal adult structures. This process is likely involve a combination cell rearrangement compensatory proliferation. However, detailed mechanisms underlying these processes are poorly understood. We have established system temporally restricted induction death in situ. Using Gal4/Gal80 UAS-rpr constructs, targeted ablation...
Abstract The Catalan Initiative for the Earth BioGenome Project (CBP) is an EBP-affiliated project network aimed at sequencing genome of >40 000 eukaryotic species estimated to live in Catalan-speaking territories (Catalan Linguistic Area, CLA). These represent a biodiversity hotspot. While covering less than 1% Europe, they are home about one fourth all known European species. include high proportion endemisms, many which threatened. This trend likely get worse as effects global...
One of the most important questions in regenerative biology is to unveil how and when genes change expression trigger regeneration programs. The resetting gene patterns during response injury governed by coordinated actions genomic regions that control activity multiple sequence-specific DNA binding proteins. Using genome-wide approaches interrogate chromatin function, we here identify elements regulate tissue recovery Drosophila imaginal discs, which show a high capacity after genetically...
In vitro DNA amplification followed by oligonucleotide mismatch hybridization was used to study the frequency of HRAS mutations in benign self-regressing skin tumors keratoacanthomas and squamous cell carcinomas. We freshly obtained as well Formalin-fixed paraffin-embedded tissues from both types tumors. 50 samples each tumor type analyzed for activating involving codons 12 61. A relatively high percentage (30%) found compared with 13% The most frequent mutation identified is A-T-to-T.A...
Selenoproteins are proteins that incorporate selenocysteine (Sec), a nonstandard amino acid encoded by UGA, normally stop codon. Sec synthesis requires the enzyme Selenophosphate synthetase (SPS or SelD), conserved in all prokaryotic and eukaryotic genomes encoding selenoproteins. Here, we study evolutionary history of SPS genes, providing map selenoprotein function spanning whole tree life. is itself many species, although functionally equivalent homologs replace site with cysteine (Cys)...
How cells communicate to initiate a regenerative response after damage has captivated scientists during the last few decades. It is known that one of main signals emanating from injured Reactive Oxygen Species (ROS), which propagate surrounding tissue trigger replacement missing cells. However, link between ROS production and activation signaling pathways not yet fully understood. We describe here non-autonomous sensing mechanism by living launch their program. To this aim, we used...
Tumor necrosis factor receptors (TNFRs) control pleiotropic pro-inflammatory functions that range from apoptosis to cell survival. The ability trigger a particular function will depend on the upstream cues, association with regulatory complexes, and downstream pathways. In Drosophila melanogaster, two TNFRs have been identified, Wengen (Wgn) Grindelwald (Grnd). Although several reports associate these JNK-dependent apoptosis, it has recently found Wgn activates variety of other functions. We...
Abstract Background Despite its pervasiveness, the genetic basis of adaptation resulting in variation directly or indirectly related to temperature (climatic) gradients is poorly understood. By using 3-fold replicated laboratory thermal stocks covering much physiologically tolerable range for temperate (i.e., cold tolerant) species Drosophila subobscura we have assessed whole-genome transcriptional responses after three years adaptation, when populations had already diverged inversion...
Abstract Background Regeneration is the ability of an organism to rebuild a body part that has been damaged or amputated, and can be studied at molecular level using model organisms. Drosophila imaginal discs, which are larval primordia adult cuticular structures, capable undergoing regenerative growth after transplantation in vivo culture into abdomen. Results Using expression profile analyses, we behaviour wing discs 0, 24 72 hours fragmentation implantation females. Based on level,...
H3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and known to be important for transcription activation. The way in which regulated TSS actual molecular basis its contribution remain largely unanswered. To address these questions, we have analyzed dKDM5/LID, main demethylase Drosophila , regulation pattern H3K4me3. ChIP-seq results show that, developmental genes, dKDM5/LID localizes regulates target are highly transcribed enriched...
The mammalian liver exhibits remarkable regenerative capabilities after injury or resection. Central to this process is the precise modulation of gene expression, driven by changes in chromatin structure and temporal activation distal regulatory elements. In study, we integrated accessibility transcriptomic data partial hepatectomy mice. We show that expression crucial regeneration genes orchestrated a diverse array cis-regulatory elements, including regeneration-specific enhancers...
An important mechanism for gene regulation involves chromatin changes via histone modification. One such modification is H3 lysine 4 trimethylation (H3K4me3), which requires methyltranferase complexes (HMT) containing the trithorax-group (trxG) protein ASH2. Mutations in ash2 cause a variety of pattern formation defects Drosophila wing. We have identified genome-wide binding ASH2 wing imaginal discs using immunoprecipitation combined with sequencing (ChIP-Seq). Our results show that genes...
PTOV1 is an adaptor protein with functions in diverse processes, including gene transcription and translation, whose overexpression associated a higher proliferation index tumor grade prostate cancer (PC) other neoplasms. Here we report its interaction the Notch pathway involvement PC progression. Stable knockdown or were performed by lentiviral transduction. Protein interactions analyzed co-immunoprecipitation, pull-down and/or immunofluorescence. Endogenous expression was real time RT-PCR...