A5043635916- Lipid metabolism and biosynthesis
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Enzyme Catalysis and Immobilization
- Chromatin Remodeling and Cancer
- Neuroblastoma Research and Treatments
- Cancer-related gene regulation
- Photosynthetic Processes and Mechanisms
- RNA modifications and cancer
- DNA Repair Mechanisms
- interferon and immune responses
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Cancer Mechanisms and Therapy
- Metabolomics and Mass Spectrometry Studies
- Lipid Membrane Structure and Behavior
Stratford University
2025
Stanford University
2024-2025
University of Chicago
2019-2022
St. Olaf College
2014-2018
Alcoholic fatty liver disease (AFLD) is characterized by an abnormal accumulation of lipid droplets (LDs) in the liver. Here, we explore composition hepatic LDs a rat model AFLD. Five to seven weeks alcohol consumption led significant increases triglyceride mass, along with LD number and size. Additionally, from rats early alcoholic injury show decreased ratio surface phosphatidylcholine (PC) phosphatidylethanolamine (PE). This occurred parallel increase association perilipin 2, prominent...
Abstract The fatal Diffuse Midline Gliomas (DMG) are characterized by an undruggable H3K27M mutation in H3.1 or H3.3. K27M impairs normal development stalling of differentiation. As replication timing influences gene expression, cell fate, and cellular response to therapeutics, we undertook a multi-omics approach (Repli-seq, cycle RNA-seq, single RNA-seq) DMG cells (H3.1K27M H3.3K27M subgroups) tumors comparison brain gain appreciation for targetable pathways DMG. presented differential each...
Rhabdoid tumors, characterized and driven by the loss of mSWI/SNF (mammalian SWItch/Sucrose Non-Fermentable) subunit SMARCB1, are very aggressive childhood cancers that can arise in brain, kidney, or soft tissues. Cell lines derived from these tumors specifically sensitivity to translation inhibitor homoharringtonin (HHT). Having recently demonstrated roles translation, we assessed SMARCB1 potential rhabdoid tumor cells. We first revealed cell viability assays cells' HHT were dependent on...
Ten-Eleven-Translocation 5-methylcytosine dioxygenases 1–3 (TET1-3) convert to 5-hydroxymethylcytosine (5-hmC), using oxygen as a co-substrate. Contrary expectations, hypoxia induces 5-hmC gains in MYCN-amplified neuroblastoma (NB) cells via upregulation of TET1. Here, we show that MYCN directly controls TET1 expression normoxia, and hypoxia, HIF-1 augments protein stability. Through gene-editing, identify two binding sites within regulate gene expression. Bioinformatic analyses distribution...
Abstract Background The fatal diffuse midline gliomas (DMG) are characterized by an undruggable H3K27M mutation in H3.1 or H3.3. K27M impairs normal development stalling differentiation. identification of targetable pathways remains very poorly explored. Toward this goal, we undertake a multi-omics approach to evaluate replication timing profiles, transcriptomics, and cell cycle features DMG cells from both H3.1K27M H3.3K27M subgroups perform comparative, integrative data analysis with...
Excess lipid is stored in an intracellular organelle known as the droplet. While core of droplet rich neutral lipids, surface primarily comprised two phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE). The further decorated with proteins, such perilipin 2, which facilitate storage utilization. Here, we show that changes to phospholipid composition alters protein binding We utilize studies synthetic droplets demonstrate reduced ratios PC:PE increase 2 vitro . Moreover,...
Lipid droplets are cytoplasmic organelles that store excess lipid. Each lipid droplet consists of a core neutral surrounded by phospholipid monolayer. Proteins interact with the surface and regulate synthesis degradation. We have shown composition synthetic can influence protein binding in an vitro assay. aimed to determine if is influenced cells. altered NIH3T3 cells hexadecylphosphocholine (HePC), inhibitor phosphatidylcholine (PC) synthesis. Isolation from treated control separation...
Excess lipid is stored in organelles known as droplets. The surface of a droplet phospholipid monolayer composed primarily two phospholipids, phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Specific proteins decorate the each regulate its activity. We aimed to determine whether composition influences number type proteins. cultured NIH 3T3 cells choline deficient media measured decrease PC:PE ratio fractions. While total levels were not changed, altered binding subset For example,...