A5026913897- CRISPR and Genetic Engineering
- Nanowire Synthesis and Applications
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
Université Laval
2021-2023
Centre hospitalier universitaire de Québec
2021-2023
Abstract Prime editing enables the introduction of precise point mutations, small insertions, or short deletions without requiring donor DNA templates. However, efficiency remains a key challenge in broad range human cell types. In this work, we design robust co-selection strategy through coediting ubiquitous and essential sodium/potassium pump (Na + /K ATPase). We readily engineer highly modified pools cells clones with homozygous modifications for functional studies minimal pegRNA...
ABSTRACT Chimeric antigen receptors (CARs) reprogram T cells to recognize and target cancer cells. Despite remarkable responses observed with CAR-T cell therapy in patients hematological malignancies, engineering still relies mostly on randomly integrating vectors, limiting the possibilities of fine-tuning function. Here, we designed a CRISPR-based marker-free selection strategy simultaneously therapeutic transgene gain-of-function mutation MTOR locus enrich resistant rapamycin, clinically...
ABSTRACT Prime editing enables the introduction of precise point mutations, small insertions, or short deletions without requiring donor DNA templates. However, efficiency remains a key challenge in broad range human cell types. In this work, we designed robust coselection strategy through coediting ubiquitous and essential sodium/potassium pump (Na + /K ATPase). We readily engineered highly modified pools cells clones with homozygous modifications for functional studies minimal pegRNA...