A5052222931- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Advanced biosensing and bioanalysis techniques
- Insect symbiosis and bacterial influences
- Monoclonal and Polyclonal Antibodies Research
- Innovation and Socioeconomic Development
- Vibrio bacteria research studies
- Fungal and yeast genetics research
- Plant Virus Research Studies
- Chemical Synthesis and Analysis
- Cytomegalovirus and herpesvirus research
- Protein Kinase Regulation and GTPase Signaling
- Mass Spectrometry Techniques and Applications
- RNA Interference and Gene Delivery
- Evolution and Genetic Dynamics
- Viral Infections and Immunology Research
- Glycosylation and Glycoproteins Research
- Bacterial biofilms and quorum sensing
- DNA and Nucleic Acid Chemistry
- Escherichia coli research studies
University of Toronto
2015-2024
National Institute of Technology Hamirpur
2020
Canada Research Chairs
2000-2018
Davidson College
2018
University of New Brunswick
2002-2015
University of Cambridge
2014
Laboratory of Molecular Genetics
1999-2011
University of Cape Town
2007
Rensselaer Polytechnic Institute
2007
Muscular Dystrophy Canada
2004
Here we report the isolation of 6 temperate bacteriophages (phages) that are prevented from replicating within laboratory strain Pseudomonas aeruginosa PA14 by endogenous CRISPR/Cas system this microbe. These phages only second identified group naturally occurring demonstrated to be blocked for replication a nonengineered system, and our results provide first evidence P. type I-F can function in phage resistance. Previous studies have highlighted importance protospacer adjacent motif (PAM)...
Most bacteriophages possess long tails, which serve as the conduit for genome delivery. We report solution structure of N-terminal domain gpV, protein comprising major portion noncontractile phage λ tail tube. This is very similar to a previously solved tube from contractile-tailed phage, providing first direct evidence an evolutionary connection between these 2 distinct types tails. A remarkable structural similarity also seen Hcp1, component bacterial type VI secretion system. The...
CRISPR-Cas systems are one of the most widespread phage resistance mechanisms in prokaryotes. Our lab recently identified first examples phage-borne anti-CRISPR genes that encode protein inhibitors type I-F system Pseudomonas aeruginosa. A key question arising from this work was whether there other types genes. In current work, we address by demonstrating some same phages carrying also possess mediate inhibition I-E P. We have discovered four distinct families these These do not inhibit...
Recent years have seen the publication of both empirical and theoretical relationships predicting rates with which proteins fold. Our ability to test refine these has been limited, however, by a variety difficulties associated comparison folding unfolding rates, thermodynamics, structure across diverse sets proteins. These include wide, potentially confounding range experimental conditions methods employed date difficulty obtaining correct complete sequence structural details for...
A library of synthetic genes encoding 80- to 100-residue proteins composed mainly random combinations glutamine (Q), leucine (L), and arginine (R) has been expressed in Escherichia coli. These also encode an epitope tag six carboxyl-terminal histidines. Screening this by immunoblotting showed that 5% these QLR are at readily detectable levels. Three well-expressed were purified characterized. Each significant alpha-helical content, is largely resistant degradation Pronase, a distinct...
A statistical analysis method can identify short, functionally important linear motifs in disordered regions of proteins.