A5071137776- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Brucella: diagnosis, epidemiology, treatment
- Infectious Diseases and Tuberculosis
- Immune responses and vaccinations
- Pneumonia and Respiratory Infections
- vaccines and immunoinformatics approaches
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 detection and testing
- Bacterial Infections and Vaccines
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Leptospirosis research and findings
- COVID-19 epidemiological studies
- Acute Ischemic Stroke Management
- COVID-19 diagnosis using AI
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- Escherichia coli research studies
- Burkholderia infections and melioidosis
- Monoclonal and Polyclonal Antibodies Research
- Diagnosis and treatment of tuberculosis
- Atherosclerosis and Cardiovascular Diseases
- Animal Disease Management and Epidemiology
- Heat shock proteins research
Central India Institute of Medical Sciences
2015-2025
Australian Institute of Tropical Health and Medicine
2021
James Cook University
2021
In the present study, we aimed to estimate occurrence of bovine tuberculosis (TB) and examine determinants distribution disease in three high-risk populations Central India.A prospective cohort study was conducted India between March 2014 June 2015.Based on requisite inclusion criteria, recruited a total 301 participants whose blood samples were subjected polymerase chain reaction-based detection differentiation Mycobacterium bovis tuberculosis.M. detected 11.4%, 8.9%, 12.6% belonging...
The COVID-19 pandemic showcased the power of genomic sequencing to tackle emergence and spread infectious diseases. However, metagenomic total microbial RNAs in wastewater has potential assess multiple diseases simultaneously yet be explored.A retrospective RNA-Seq epidemiological survey 140 untreated composite samples was performed across urban (n = 112) rural 28) areas Nagpur, Central India. Composite were prepared by pooling 422 individual grab collected prospectively from sewer lines...
<title>Abstract</title> This study aimed to develop a cost-effective middle cerebral artery occlusion (MCAO) model in rats using an intraluminal suture method with magnifying glass lens, alternative surgical microscope. Male Sprague-Dawley underwent MCAO surgery, and neurological deficits were assessed post-surgery. Neurological scores significantly higher (mean score 3.5 ± 0.5) compared sham-operated controls 0.5 0.2) (p < 0.001). 2,3,5-Triphenyltetrazolium chloride (TTC) staining...
Background The present study was designed to investigate the utility of Quantiferon TB gold (QFT-G) and Tuberculin skin test (TST) for diagnosis latent infection (LTBI) in high crowding endemic zone Nagpur, India their comparison with associated risk factors. Methods Out 342 eligible participants, QFT-G TST were performed 162 participants. Results prevalence LTBI observed according 48% 42% respectively, an agreement 52.47%. positivity age while body mass index (BMI). Duration exposure...
Brucellosis is a neglected zoonotic disease of major public health concern. In India, the incidence brucellosis remains vastly underreported due to its non-specific clinical presentation and sub-optimal sensitivity existing gold standard tests. Studies in Northeast India have shown high incidences livestock, but region lacks data on human despite associated risk. present study, we report seroprevalence risk factors Meghalaya, India.A prospective observational study was conducted East Khasi...
Lack of diagnostic capacity has been a crucial barrier preventing an effective response to the challenges malnutrition and tuberculosis (TB). Point-of-care tests for TB in immuno-incompetent, malnourished population are thus needed ensure rapid accurate detection. The aim study was identify potential biomarkers specific infection progression overt disease Melghat. A prospective cohort conducted year 2009 through 2011 six villages Melghat region. 275 participants consisting cases with a)...
Background: Bacterial meningitis (BM) is severe complication of central nervous system (CNS) and often associated with high mortality morbidity rates if not timely diagnosed treated. Current diagnostic tools for BM drug resistance suffer from lack sensitivity due to paucibacillary nature Cerebrospinal fluid (CSF).
 Objectives: The objective the study develop rapid efficacious immunological (Enzyme linked immunosorbent assay Lateral flow) diagnosis in hospital settings. In addition,...
India has a higher tuberculosis (TB) burden than any other country, accounting for an estimated one-fourth of the global burden. Drug-resistant (DR-TB) presents major public health problem in India. Patients with DR-TB often require profound changes their drug regimens, which are invariably linked to poor treatment adherence and sub-optimal outcomes compared drug-sensitive TB. The challenge addressing is critical India, as contributes over 27% cases. In recent decades, been proactive its...
Background: Latent TB infection (LTBI) is an where the presence of disease causing organism M. tuberculosis there without any sign and symptoms hence mostly remains undiagnosed, though Tuberculin skin test (TST) Interferon Gamma Release Assay (IGRA) were used to diagnose LTBI. They have their limitations, TST gives major cross-reactivity with BCG vaccine inaccurate results in individuals who taken IGRA are very costly variable sensitivity repeated various populations modifications needed for...
Tuberculosis (TB) is one of the most prevalent cause death due to a single pathogen. Bacillus Calmette Guérin (BCG) only vaccine available for clinical use that protects against miliary TB; however, this has shown variable levels efficacy pulmonary TB. In India, dose BCG given and there are few countries where repeated doses given. The incidence TB in India very high inspite primary vaccination neonatal period therefore requires consideration immunization. To improve immunogenicity, we have...
Background Malnutrition is a major risk factor for the development of tuberculosis (TB). In India, Melghat among tribal regions which consist highest number malnutrition cases. Because paucity TB data from these malnourished areas there an urgent need and evaluation improved diagnostic tests. present study, three in house developed tests namely TB-Ag(antigen) ELISA, Adenosine deaminase (ADA) estimation IS6110 polymerase chain reaction (PCR) assay were investigated detection Mycobacterium (M....
The present study was designed to investigate Rv2623 antigen, a major dormancy regulon protein of Mycobacterium tuberculosis (MTB) in CSF suspected latent and active tuberculous meningitis (TBM) patients. A total 100 samples from TBM (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>31</mml:mn></mml:math>), id="M2"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn>22</mml:mn></mml:math>), suitable noninfectious control subjects...
In the present study booster efficacies of Ag85 B, Bacillus Calmette-Guerin (BCG), and Ag85B peptides were evaluated using prime boost regimes in BALB/c mice. Mice primed with BCG vaccine subsequently boosted Ag85B, cocktail peptides. Based on analysis immune response it was observed mice showed significant (p < 0.001) cytokines levels (interferon γ, interleukin 12) specific antibodies (anti-BCG anti-purified protein derivative titre) compared to dose BCG, alone. Our pilot results suggest...
Central nervous system (CNS) infection caused by Mycobacterium tuberculosis (MTB) is the most severe form of extrapulmonary (EPTB) due to a high level mortality and morbidity. Limited studies are available on CNS-TB animal model development. The present study describes development murine using clinical strain (C3) isolated from cerebrospinal fluid (CSF) patients.Groups mice were infected intravenous route with MTB C3 CSF patients. Brain lung tissue evaluated for bacterial burden,...
Purpose: In the present study, protective immunological markers in serum and peripheral blood mononuclear cells (PBMCs) of bacillus Calmette-Guérin (BCG) vaccinated unvaccinated children were evaluated after vaccination.Further, PBMCs with low levels boosted BCG, Ag85B, Ag85B peptides to study their booster effects increase waning BCG induced immunity.Materials Methods: Fifty from 1 month 18 years age randomized for study.Blood samples collected 27 participants with/without...
Human brucellosis is a neglected zoonotic disease of significant public health concern. Molecular diagnosis brucella remains challenging in low resource settings, due to the high infrastructure and cost involved. Loop-mediated isothermal amplification (LAMP) rapid point care polymerase chain reaction (PCR) with utility on-field molecular offers convenient alternative conventional PCR. In present study, we developed evaluated diagnostic house LAMP PCR targeting Brucella genus-specific bcsp-31...
Human brucellosis is an important zoonotic disease of public health and often remains neglected owing to lack sensitive efficient diagnostic methods. This study evaluates utility in-house designed enzyme-linked immunosorbent assay (ELISA) using whole-cell antigens Brucella abortus (B. abortus) S19 against the commercially available kits.A prospective cohort involving different populations within Vidarbha regions Maharashtra, India was conducted through camps organised from May 2009 October...
Brucellosis is an important zoonotic disease and has public health importance. In the present study, we studied prevalence associated risk factors of human brucellosis in central Indian population from tertiary care settings. A prospective observational study was conducted from March 2015 to February 2018 in patients attending outpatient department (OPD) Central India Institute Medical Sciences (CIIMS), Nagpur. A total 7026 individuals suspected brucellosis were recruited based on...
Evaluation and screening of vaccines against tuberculosis depends on development proper cost effective disease models along with identification different immune markers that can be used as surrogate endpoints protection in preclinical clinical studies. The objective the present study was therefore evaluation subcutaneous model M.tuberculosis infection investigation biomarkers BALB/c mice. Groups mice were infected subcutaneously two doses : high (2×10(6) CFU) low (2×10(2) including humoral...