A5058092197- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Studies on Chitinases and Chitosanases
- Environmental DNA in Biodiversity Studies
- Lipid metabolism and biosynthesis
- Trypanosoma species research and implications
- Insect-Plant Interactions and Control
- Neurobiology and Insect Physiology Research
- Physiological and biochemical adaptations
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Insect and Arachnid Ecology and Behavior
- Mitochondrial Function and Pathology
Universidade Federal do Rio de Janeiro
2020-2024
National Institute of Allergy and Infectious Diseases
2024
National Institutes of Health
2024
Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular
2022
Abstract Background Real-time quantitative PCR (RT-qPCR) is one of the most widely used gene expression analyses for validating RNA-seq data. This technique requires reference genes that are stable and highly expressed, at least across different biological conditions present in transcriptome. Reference variable candidate selection often neglected, leading to misinterpretation results. Results We developed a software named “Gene Selector Validation” (GSV), which identifies best validation...
Abstract Background Hemocytes are immune cells that patrol the mosquito hemocoel and mediate critical cellular defense responses against pathogens. However, despite their importance, a comprehensive transcriptome of these was lacking because they constitute very small fraction total in insect, limiting study hemocyte differentiation function. Results In this study, an in-depth built by extensive bulk RNA sequencing assembly RNAs from adult A. gambiae female mosquitoes, based on approximately...
Zika virus (ZIKV) is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children adults. A total of 87 countries have had evidence autochthonous mosquito-borne transmission ZIKV, distributed across four continents, no antivirus therapy or vaccines are available. Therefore, several strategies been developed target the main mosquito vector, Aedes aegypti , reduce burden different arboviruses. Among such strategies,...
Abstract In the present work, we established two novel embryonic cell lines from mosquito Aedes fluviatilis containing or not naturally occurring symbiont bacteria Wolbachia, which were called w Aflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate symbiont-host interaction in context of energy metabolism regulation molecular mechanisms immune responses...
The interaction between Rhodnius prolixus and Trypanosoma cruzi has huge medical importance because it responds to the transmission of Chagas disease, a neglected tropical disease that affects about eight million people worldwide. It is known trypanosomatid pathogens depend on active lipid endocytosis from insect host meet growth differentiation requirements. However, until now, knowledge how parasite physiology individual organs was largely unknown. Herein, biochemical molecular dynamics...
The adult females of Aedes aegypti mosquitoes are facultative hematophagous insects but they unable to feed on blood right after pupae emergence. maturation process that takes place during the first post-emergence days, hereafter named hematophagic and gonotrophic capacitation, comprises a set molecular physiological changes prepare for cycle. Notwithstanding, bases underlying mosquito capacitation remain obscure. Here, we investigated biochemical in Ae . along four days post-emergence,...
Carbohydrate metabolism not only functions in supplying cellular energy but also has an important role maintaining physiological homeostasis and preventing oxidative damage caused by reactive oxygen species. Previously, we showed that arthropod embryonic cell lines have high tolerance to H2O2 exposure. Here, describe Rhipicephalus microplus tick line (BME26) employs adaptive glucose mechanism confers hydrogen peroxide at concentrations too for other organisms. This sustained remodeling...
ABSTRACT Zika virus is a global public health emergency due to its association with microcephaly, Guillain-Barré syndrome, neuropathy, and myelitis in children adults. A total of 87 countries have had evidence autochthonous mosquito-borne transmission virus, distributed across four continents, no antivirus therapy or vaccines are available. Therefore, several strategies been developed target the main mosquito vector, Aedes aegypti , reduce burden different arboviruses. Among such strategies,...
Abstract In the present work, we established two novel embryonic cell lines from mosquito Aedes fluviatilis containing or not naturally occurring symbiont bacteria Wolbachia, which were called w Aflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate symbiont-host interaction in context of energy metabolism regulation molecular mechanisms immune responses...