A5016230542- Dental materials and restorations
- Dental Erosion and Treatment
- Natural product bioactivities and synthesis
- Laser Applications in Dentistry and Medicine
- Bioactive natural compounds
- Endodontics and Root Canal Treatments
- Oral microbiology and periodontitis research
- Biological Activity of Diterpenoids and Biflavonoids
- Bone and Dental Protein Studies
- Biological Stains and Phytochemicals
- Chromatography in Natural Products
- Recycling and utilization of industrial and municipal waste in materials production
- Collagen: Extraction and Characterization
- Phytochemicals and Antioxidant Activities
- Analytical Chemistry and Chromatography
- Synthesis of Organic Compounds
- Therapeutic Uses of Natural Elements
- Medicinal Plants and Bioactive Compounds
- Silicone and Siloxane Chemistry
- Pain Management and Placebo Effect
- Marine Sponges and Natural Products
- Biological and pharmacological studies of plants
- Dermatologic Treatments and Research
- Calcium Carbonate Crystallization and Inhibition
- Structural Analysis of Composite Materials
University of Illinois Chicago
2019-2024
Marquette University
2020-2023
University of Illinois Urbana-Champaign
2020
Universidade de São Paulo
2011-2018
In nature, proanthocyanidins (PACs) with A-type linkages are relatively rare, likely due to biosynthetic constraints in the formation of additional ether bonds be introduced into more common B-type precursors. However, confer greater structural rigidity on PACs than do linkages. Prior investigations structure–activity relationships (SAR) describing how plant-derived B- and complex AB-type affect their capacity for dentin biomodification indicate that a higher ratio double leads interaction...
A dentin biomodification strategy with selective proanthocyanidin (PAC)–enriched extracts reinforces and dentin-resin interfaces. Enrichment of the according to degree polymerization allows exploration bioactive principles PACs structure-activity relationships. This study investigated sustained matrix bioadhesion 2 fractions consisting exclusively B-type PAC dimers or without a single galloyl motif (specifically, DIMER G NG ) their precursor material, enriched grape seed extract (e-GSE;...
Guided by dentin biomechanical bioactivity, this phytochemical study led to the elucidation of an extended set structurally demanding proanthocyanidins (PACs). Unambiguous structure determination involved detailed spectroscopic and chemical characterization four A-type dimers (2 4-6), seven trimers (10-16), six tetramers (17-22). New outcomes confirm feasibility determining absolute configuration catechol monomers in oligomeric PACs one-dimensional (1D) two-dimensional (2D) NMR. Electronic...
To enable translational studies, a scalable preparative isolation scheme was developed for underivatized cocoa (Theobroma cacao) proanthocyanidins (PACs), affording six all-B-type oligomeric PACs, including new tetramer 4. Their structures, absolute configuration, were unambiguously established by comprehensive spectroscopic and chemical methods. Evaluation of the PACs' dentin biomodification properties employed dynamic mechanical infrared analyses in bioassay models. PAC treatment enhanced...
To enable the further exploration of structure–activity relationships (SARs) proanthocyanidins (PACs) with dentin biomodification abilities, Cinnamomum verum was selected for scaled-up purification mixed A-/B-type, medium-size PAC oligomers. Sequential by centrifugal partition chromatography (CPC), Sephadex LH-20, and semiprep HPLC yielded four underivatized tetrameric (5–8) two pentameric (9–10) PACs. Their unambiguous structural characterization involved extensive spectral chemical...
An investigation of the dental bioactive proanthocyanidin (PAC) oligomer fractions led to three structurally distinct new PACs (
The use of polymer additives that stabilize fluidic amorphous calcium phosphate is key to obtaining intrafibrillar mineralization collagen in vitro. On the other hand, this biomimetic approach inhibits nucleation mineral crystals unconfined extrafibrillar spaces, is, mineralization. content a significant feature replicate from hard connective tissues such as bone and dentin it contributes final microarchitecture mechanical stiffness biomineral composite. Herein, we report straightforward...
Abstract Flavan‐3‐ol monomers are the building blocks of proanthocyanidins (PACs), natural compounds from plants shown to mediate specific biologic activities on dentin. While stereochemistry terminal flavan‐3‐ols, catechin (C) versus epicatechin (EC), impacts biomechanical properties dentin matrix treated with oligomeric PACs, structure–activity relationships driving this bioactivity remain elusive. To gain insights into modulatory role monomers, two highly congruent trimeric PACs Pinus...
Objective: the aim of this study was to assess effects whitening dentifrices on surface roughness three commercial nanofiller-based composite. Material and Methods: two optical analyses were performed: stereomicroscopy atomic force microscopy (AFM). Disks (8.0 diam. x 2.0 mm thick.) nanocomposite Z350 (3M ESPE) nano-hybrid composites (EsthetXHD, Dentsply Premisa, Kerr) submitted in vitro brushing (1000 cycles) with (Colgate Luminous White, Oral-B 3D White e Close-Up Diamond Attraction) a...
Proanthocyanidins (PACs) are complex flavan-3-ol polymers with stunning chemical complexity due to oxygenation patterns, oxidative phenolic ring linkages, and intricate stereochemistry of their heterocycles inter-flavan linkages. Being promising candidates for dental restorative biomaterials, trace analysis dentin bioactive cinnamon PACs now yielded novel trimeric (1 2) tetrameric (3) unprecedented o- p-benzoquinone motifs (benzoquinonoid PACs). Challenges in structural characterization,...
Abstract Plant‐derived proanthocyanidins (PACs) mediate physicochemical modifications to the dentin extracellular matrix (ECM). The structure–activity relationships of PACs remain largely unknown, mostly due varied complex composition crude extracts, as well challenges purification and mechanistic assessment. To assess role galloylated significant contributors high yet unstable biomodification activity ECM, we removed galloyl moieties (de‐galloylation) via enzymatic hydrolysis from three...
Abstract Objective Devise a pipeline to investigate the protein composition of human root dentin extracellular matrix (ECM) from single individuals different age cohorts. Design Individual cervical sound molars two brackets, young (18-25 years old; n=3) and old (75-85 n=3), were cut pulverized. Protein extraction fractionation completed by sequential demineralization with EDTA buffer, chaotropic guanidine hydrochloride, urea. The resulting extracts differential solubility digested into...
Aim: This work evaluated dental porcelain sintering by CO2 laser irradiation. Methods: Two commercial veneering porcelains were used: VM7 and VM9 (VITA Zahnfabrik). Porcelain disks (0.35 mm dia. x 2.0 mm) sintered a continuous (Coherent, USA – 35 W e ?= 10.6 ?m) in two irradiances (20 23 W/cm2) exposure times: 5 10 min. A control group was conventional oven following manufacturer’s instructions. After sintering, one of the surfaces mirror polished. Superficial pores (%) assessed images...
Guided by dentin biomechanical bioactivity, this phytochemical study led to the elucidation of an extended set structurally demanding proanthocyanidins (PACs). Unambiguous structure determination involved detailed spectroscopic and chemical characterization four A-type dimers (2, 4–6), seven trimers (10–16), six tetramers (17–22). New outcomes confirm feasibility determining absolute configuration catechol monomers in oligomeric PACs 1D 2D NMR. Electronic circular dichroism (ECD) as well...