A5001301880- Protist diversity and phylogeny
- Microtubule and mitosis dynamics
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Hippo pathway signaling and YAP/TAZ
- Malaria Research and Control
- Enzyme Structure and Function
- Microbial Community Ecology and Physiology
- Geomagnetism and Paleomagnetism Studies
- Plant Parasitism and Resistance
- Toxin Mechanisms and Immunotoxins
- Hereditary Neurological Disorders
- Cellular transport and secretion
- Fungal and yeast genetics research
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Bacterial biofilms and quorum sensing
- Ecology and Vegetation Dynamics Studies
- Biomedical Research and Pathophysiology
University of Technology Sydney
2020-2024
University of Georgia
2023
The University of Melbourne
2019
Apicomplexan parasites possess a plastid organelle called the apicoplast. Inhibitors that selectively target apicoplast housekeeping functions, including DNA replication and protein translation, are lethal for parasite, several (doxycycline, clindamycin, azithromycin) in clinical use as antimalarials. A major limitation of such drugs is treated only arrest one intraerythrocytic development cycle (approximately 48 hours) after treatment commences, phenotype known 'delayed death' effect. The...
Some microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt changed environmental conditions. The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped (‘plate’) cells but may also be present as rods, and can develop into motile rods soft agar, or longer filaments certain biofilms. Here we report improvement of H. growth both semi-defined complex media by supplementing with eight trace element micronutrients....
Archaeal cell biology is an emerging field expected to identify fundamental cellular processes, help resolve the deep evolutionary history of life, and contribute new components functions in biotechnology synthetic biology. To facilitate these, we have developed plasmid vectors that allow convenient cloning production proteins fusion with flexible, rigid, or semi-rigid linkers model archaeon Haloferax volcanii . For protein subcellular localization studies using fluorescent (FP) tags,...
Abstract Archaeal CetZ cytoskeletal proteins are a major group of the tubulin superfamily involved in development motile rod-shaped cells. In model archaeon Haloferax volcanii , it has been unknown whether CetZs contribute to motility solely through effects rod cell shape on swimming speed, or other ways too. Here, we used cryo-electron and fluorescence microscopy observe surface filaments assembly machinery cells carrying cetZ1 cetZ2 deletion, overexpression, dominant GTPase-deficient...
Tubulin superfamily (TSF) proteins are widespread, and known for their multifaceted roles as cytoskeletal underpinning many basic cellular functions, including morphogenesis, division, motility. In eukaryotes, tubulin assembles into microtubules, a major component of the dynamic network fibres, whereas bacterial homolog FtsZ division ring at midcell. The functions lesser-known archaeal TSF beginning to be identified show surprising diversity, homologs well third archaea-specific family,...
Abstract Tubulin superfamily (TSF) proteins include the well-known eukaryotic tubulin and bacterial FtsZ families, lesser-known archaeal CetZ family. In eukaryotes bacteria, GTP-dependent polymerization self-association of protofilaments are integral to formation cytoskeletal structures with essential roles in cell division, growth, morphology. Archaeal CetZs implicated control shape motility through unknown mechanisms. Here, we reveal a sequence subcellular localization patterns CetZ1,...
Abstract Archaeal cell biology is an emerging field expected to identify fundamental cellular processes, help resolve the deep evolutionary history of life, and contribute new components functions in biotechnology synthetic biology. To facilitate these, we have developed plasmid vectors that allow convenient cloning production proteins fusion with flexible, rigid, or semi-rigid linkers model archaeon Haloferax volcanii . For protein subcellular localization studies using fluorescent (FP)...
Abstract Some microbes display pleomorphism, showing variable cell shapes in a single culture, whereas others differentiate to adapt changed environmental conditions. The pleomorphic archaeon Haloferax volcanii commonly forms discoid-shaped (‘plate’) cells but may also be present as rods, and can develop into motile rods soft agar, or longer filaments certain biofilms. Here we report improvement of H. growth both semi-defined complex media by supplementing with eight trace-element...
Abstract CetZ proteins are archaea-specific homologues of the cytoskeletal FtsZ and tubulin. In pleomorphic archaeon Haloferax volcanii , CetZ1 contributes to development rod shape motility, has been implicated in proper assembly positioning archaellum chemotaxis motility proteins. shows complex subcellular localization, including irregular midcell structures filaments along long axis developing rods patches at cell poles motile type. The polar localizations also influenced by MinD4, only...
Abstract CetZs are archaeal tubulin superfamily cytoskeletal proteins implicated in the control of cell shape and motility. In pleomorphic archaeon Haloferax volcanii , CetZ1 is required for transformation a discoid, or plate-like, morphology to rod during early-mid log phase cultures development swimming this study, we found that paralog CetZ2 not reversion plates but was strongly upregulated later stationary phase, where it promotes maintenance plate shape. structures visualized through...
Abstract Increased efforts to use native plants provide ecosystem services in urban landscapes have led breeding increase access horticulture markets. Inadvertent ecological impacts however should be assessed prior market release. Here we trial new interspecific hybrids of milkweed assess outcrossing into natural populations and support for pollinating species. Interspecific Asclepias were unable successfully fertilize A. tuberosa, indicating minimal likelihood populations. Hybrids lacked...
CetZ proteins are archaea-specific homologs of the cytoskeletal FtsZ and tubulin. In pleomorphic archaeon Haloferax volcanii , CetZ1 contributes to development rod shape motility, has been implicated in proper assembly positioning archaellum chemotaxis motility proteins. shows complex subcellular localization, including irregular midcell structures filaments along long axis developing rods patches at cell poles motile type. The polar localizations also influenced by MinD4, only previously...
Tubulin superfamily (TSF) proteins are widespread and known for multifaceted roles as cytoskeletal underpinning many basic cellular functions including morphogenesis, division, motility. In eukaryotes, tubulin assembles into microtubules, a major component of the dynamic network fibres, whereas bacterial homolog FtsZ division ring at midcell. Functions lesser-known archaeal TSF beginning to be identified, show surprising diversity, homologs FtsZ, third archaea-specific family, CetZ,...