A5010358245- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- DNA and Nucleic Acid Chemistry
- Nanocluster Synthesis and Applications
- Molecular Sensors and Ion Detection
- Advanced Nanomaterials in Catalysis
- Analytical Chemistry and Chromatography
- Peptidase Inhibition and Analysis
- Gold and Silver Nanoparticles Synthesis and Applications
- Neuropeptides and Animal Physiology
- GABA and Rice Research
- Analytical Methods in Pharmaceuticals
- Antibiotics Pharmacokinetics and Efficacy
- RNA Interference and Gene Delivery
- bioluminescence and chemiluminescence research
- Protease and Inhibitor Mechanisms
- Inflammatory mediators and NSAID effects
- Advancements in Battery Materials
- Angiogenesis and VEGF in Cancer
- Conducting polymers and applications
- Supercapacitor Materials and Fabrication
- Surfactants and Colloidal Systems
- Solar Thermal and Photovoltaic Systems
- Solar-Powered Water Purification Methods
Fudan University
2015-2025
Minhang District Central Hospital
2019-2025
The First Affiliated Hospital, Sun Yat-sen University
2024
Zhongshan Hospital
2024
Sun Yat-sen University
2024
First Affiliated Hospital of GuangXi Medical University
2017
Guangxi Medical University
2017
University of Florida Health
2017
Interface (United States)
2017
Tianjin University
2016
Disease-related biomarkers are objectively measurable molecular signatures of physiological status that can serve as disease indicators or drug targets in clinical diagnosis and therapy, thus acting a tool support personalized medicine. For example, the prostate-specific antigen (PSA) biomarker is now widely used to screen patients for prostate cancer. However, few such currently available, process identification validation prolonged complicated by inefficient methods discovery reliable...
A self-floating photothermal membrane with simultaneous mechanical stability and antibacterial activity is facilely prepared for efficient solar-driven interfacial water evaporation.
Bacterial determination, emerging as a critical step in the understanding of increasingly serious bacterial contaminations, remains major challenge. Herein, novel chemiluminescence biosensor was exploited for ultrasensitive determination nuclease activity and bacteria, which, hemin, chemiluminescent (CL) tag molecule encapsulated into ordered mesopores mesoporous silica nanoparticles with specific DNA gate. The capped could be specifically switched upon exposure to or lysate allowed an...
Quantum dots (QDs) have the potential to simplify performance of multiplexed analysis. In this work, a novel protocol for performing simultaneous dual-protein immunoassay, i.e. two lung cancer biomarkers, carcinoembryonic antigen (CEA) and neuron-specific enolase (NSE), based on dual-color QDs, is described. First, capture antibodies (both with biotin tags), antigens detection were mixed together sandwich complexes thus formed in homogeneous solution, then streptavidin coated polystyrene...
We introduce here a novel assay for the detection of platelet-derived growth factor BB (PDGF-BB) via hybridization chain reaction (HCR) based on an aptameric system, where stable DNA monomers assemble only upon exposure to target PDGF-BB aptamer. In this process, two complementary species biotinylated hairpins coexist in solution until introduction initiator aptamer strands triggers cascade events that yields nicked double helices analogous alternating copolymers. detail, firstly opens...
Sensitive detection and accurate diagnosis/prognosis of glioma remain urgent challenges. Herein, dispersed magnetic covalent organic framework nanospheres (MCOF) with uniformed Fe3O4 nano-assembly as cores high-crystalline COF shells were prepared by monomer-mediated in-situ interface growth strategy. Based on the unique interaction between MCOF hairpin DNA, a fluorescent signal amplified miRNA biosensor was constructed. It could realize sensitive miRNA-182 in different matrixes, where...
There are potential advantages, in terms of simplicity and speed, for detecting DNA hybridization steps directly without using any external labels, especially the multiplexed assays. In current paper, we describe use a carrier-resolved label-free assay simultaneous detection multiple targets. Herein demonstrate that this protocol, three homogeneous carriers thermosensitive poly(N-isopropylacrylamide), polystyrene beads, magnetic respectively, determination short fragments specific to...
We have developed a new aptamer-based chemiluminescence (CL) biosensing platform for the sequential detection of two small molecules as exemplified by adenosine and cocaine. Each comprises NH2-functionalized capture DNA immobilized on magnetic beads; this can hybridize with one end aptamer. A corresponding reporter probe labeled either digoxigenin or biotin its 5′-terminus recognizes other The target compounds cocaine act specific competitors to aptamer–reporter binding, aptamers...
Fibroblast activation protein-alpha (FAPα) is a key modulator of the microenvironment in multiple pathologies and becoming next pan-cancer target for cancer diagnostics therapeutics. Chemiluminescence (CL) luminophores are considered as one most sensitive families probes detection imaging applications due to their high signal-to-noise ratio. Until now, however, no such effective CL probe was reported FAPα detection. Herein, we developed novel endogenous activity by incorporating...
Being a class of highly ordered porous materials, hydrogen-bonded organic framework (HOFs) hold great potential for developing promising enzyme mimics in sensing applications, yet remain underutilized this field. Herein, robust HOF@Co/Heme with stable peroxidase-like activity was synthesized by loading Heme and Co2+ to HOF establishing sensitive chemiluminescence (CL) assay glucose. The scavenger experiment EPR test revealed that reactive oxygen species (ROS) including 1O2, OH· O2·- were...
We report on a highly sensitive aptameric assay system for the determination of IgE, where special chemiluminescence (CL) reagent, 3,4,5-trimethoxylphenylglyoxal (TMPG), acts as signaling molecule and polystyrene beads amplification platform. Briefly, "sandwich-type" detection strategy is employed in our design, magnetic functionalized with capture antibody were reacted target protein then sandwiched aptamer-barcodes which prepared by assembling IgE aptamer. The immunoreaction event could be...
A new concept is proposed in this article to detect multiple cancer markers a single sample. Quantum dot (QD) fluorescence (FL) labels were successfully combined with enzyme chemiluminescence (CL) for simultaneous detection of three human serum using just common 96-well plate reader and equal limits the markers. As proof-of-concept, herein we coupled one QD FL label two CL hybrid multiplexed lung as exemplified by neuron-specific enolase (NSE), carcinoembryonic antigen (CEA) cytokeratin...
By using the allosteric hairpin DNA switch, a novel label-free assay for detection of microRNA (miRNA) let-7a <italic>via</italic> hybridization chain reaction (HCR) was introduced, which can achieve ultrasensitivity up to approximately 0.1 fmol, <italic>i.e.</italic> 1 pM.
A simple, rapid, and accurate stability-indicating reverse phase liquid chromatographic method was developed validated for the simultaneous determination of pheniramine maleate naphazoline hydrochloride in bulk drugs pharmaceutical formulations. Optimum separations among maleate, stress-induced degradation products have been achieved within 10 minutes by using an Agilent zorbax eclipse XDB C18 column (150 mm × 4.6 mm, 5 μm) as stationary with a mobile consisted mM phosphate buffer pH 2.8...
A robust, selective and highly sensitive chemiluminescent (CL) platform for protein assay was presented in this paper. This novel CL approach utilized rolling circle amplification (RCA) as a signal enhancement technique the 96-well plate immobilization separation carrier. Typically, antibody immobilized on surface of sandwiched with target aptamer–primer sequence. sequence then employed primer RCA. Based design, number biotinylated probes streptavidin–horseradish peroxidase (SA–HRP) were...