Background and Aim: Despite the endemicity of Japanese encephalitis virus (JEV) in humans animals Province Bali, Indonesia, there is little data on whether seroconversion to occurs pigs, JEV genotypes circulating, it’s potential mosquito vectors area. The aims this study were (i) Determine infection Balinese pigs before reaching their sexual maturity, (ii) identify circulating JEV, (iii) at sites urban peri-urban areas Bali. Materials Methods: Sixteen 1-week-old Landrace piglets from two...

c-Jun is often found to be overexpressed in various cancers, so this gene might a target for cancer therapy. Newcastle disease virus (NDV) recognized its oncolytic properties and potential as virotherapy agent, with mechanisms reported trigger cell death. This study aimed assess the expression rat mammary models. Rat models were categorized into two treatment groups: control group (C) (V). Group C was administered 0.5 cc of sterile PBS, while V received 7 log 2 HAU per Indonesian NDV field...

Ultraviolet B (UVB) irradiation induces photoaging and apoptosis in various cell types. Inhibition of UVB-induced apoptotic pathways has been explored different cascades. Conditioned media from human umbilical cord blood mesenchymal stem cells (CM-hUCB-MSC) contain important substances for regeneration. However, the potential CM-hUCB-MSC preventing not clearly elucidated. Therefore, current research was conducted to investigate inhibiting its role antiapoptotic signaling pathway. An...

Hepatitis E virus (HEV) is a leading cause of acute hepatitis transmitted through the enteric route, impacting millions individuals worldwide annually. While HEV generally self-limiting, it can lead to considerable illness and death, particularly in gestating women, immunocompromised individuals, those with pre-existing conditions, such as chronic liver disease. genotypes 1 2 infect solely humans are prevalent areas poor sanitation, whereas 3 4 zoonotic, infecting both animals humans....

Caulerpa sp., a genus of seaweed native to the Indo-Pacific region, has been known for its antioxidant properties and health benefits when consumed as food. Previous studies have reported sp.'s potential strong antioxidant, but effects on skin in topical preparation, especially role ultraviolet (UV) protection, not studied extensively. Our study investigated protective 0.2% 0.4% sp. extract gels photoaging UVB-irradiated Wistar mice. The subjects were divided into naive control, vehicle 3...

Introduction Japanese encephalitis (JE) is a zoonotic disease caused by virus (JEV) infection which transmitted mosquitoes from pigs as the main amplifier host to human and other vertebrates. JEV still major cause of viral in Asia can severe

Japanese encephalitis (JE) is a zoonotic infectious inflammatory brain disease caused by the JE virus (JEV). Considerable research into seroprevalence of in domestic animals has been conducted, but there have no reports its occurrence wild animals, including long-tailed macaques (Macaca fascicularis). This study aimed to estimate JEV infection and determinants Bali prevalence mosquito vectors.Blood samples (3 mL) were collected from population M. fascicularis (92 heads) inhabiting small...

Chronic total occlusion (CTO) is an angiographic picture of without blood flow which estimated to have lasted at least 3 months. This study attempted provide overview the levels matrix metalloproteinase-9 (MMP-9), soluble suppression tumorigenicity 2 (sST2), and N-terminal pro-B-type natriuretic peptide (NT-pro-BNP) as remodeling, inflammatory, atherosclerotic markers, well changes in angina severity patients with CTO who underwent percutaneous coronary intervention (PCI) compared those...

Aim: This study aimed to prepare binary ethylenimine (BEI)-inactivated virulent Newcastle disease virus (NDV) vaccine and examine their ability induce a protective antibody response in commercial chickens. Materials Methods: A NDV field isolate Gianyar-1/AK/2014 was propagated chicken-embryonated eggs then inactivated with BEI at concentration of 4 mM. Three groups chickens low-level (2 log2 hemagglutination inhibition [HI] units) maternally derived antibodies against were immunized the...

Background: This research aims to find a cure for anti-inflammation, based on the utilization of red piper crocatum. The was started with descriptive study explore active components crocatum leaf and followed by experimental investigate activity extract in anti-inflammation induced Wistar rat. In this observed three dominant components: caryophyllene bicyclo [5.2.0] none,2 methylene-4,8,8-trimethyl-4-vinyl; phytol; 5,9-propano-5H-benzocycloheptene,6,7,8,9-tetrahydro-7,11-bis(methylene);...

The protective antibody response of Balb/c mice to Bali rabies virus (RABV) in BHK-21 cells was studied. isolated from a rabid dog and adapted replicate cell culture for seven passages. BHK-21-adapted RABV (BHK-Bali RABV) inactivated with binary ethylenimine 24 were immunized twice at 21-days intervals the Rabisin® vaccine. Virus replication detected using indirect immunofluorescence, immunocytochemistry, western blotting assays. Enzyme-linked immunosorbent assay examination 2 weeks after...

Background: Green meniran plant (Phyllanthus niruri Linn) is traditionally believed to treat many diseases, such as reducing fever, diarrhea, cough with phlegm, inflammation, malaria, and acne. This research aims determine the class of compounds active in ethanol extract green leaf.Methods: An experimental study was conducted using phytochemical testing includes qualitative quantitative tests identify leaf by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Crude powder packaging...

A study to assess the seroprevalence antibodies against JEV in pigs Denpasar, Badung, and Karangasem as representatives of urban, periurban, rural areas province Bali was conducted. Sampled pigs’ blood collected their sera were tested for antibody detection using commercial IgG ELISA. standard questionnaire used interview pig owners or farmers identify determinants associated with seropositivity antibodies. Overall, 96.6% (95% CI: 94.5–98.1) 443 individual animal‐level seropositive had...

Link of Video Abstract: https://youtu.be/XrtI29YbnTs Background: Osteoarthritis (OA) is the most common form arthritis, which cause pain and disability that can affect quality life. The inflammation in OA chronic characterized by infiltration immune cells secretion cytokines. Previous treatment used hyaluronic acid intraarticular injection, but several studies show no satisfactory clinical outcome. This study aims to determine compare anti-inflammatory anti-catabolism effects stem...

This research aimed to identify the antioxidant capacity, vitamin C (ascorbic acid), and total phenolic compounds of frangipani flower powder. The powder was extracted using ethanol, methanol, acetic acid, water (aquadest). Antioxidant capacity each extract were determined 2,2-diphenyl-1-picrylhydrazyl radical scavenging method, 2,4 Dinitrophenylhydrazine, Folin Ciocalteu reagent. All parameters measured by spectrofotometer. result shows that highest value ethanolic (18.19%) lowest acid...

Hypercholesterolemia or hyperlipidemia has been established as an important risk factor of cardiovascular disease. Patients with hypercholesterolemia usually require a prolonged treatment; and the newer more potent generation antilipid agents are costly.In Bali there several types seaweed that generally consumed by local people known names Bulung Boni (Caulerpa spp.) Sangu (Gracilaria spp.). Preliminary studies on effect extracts appeared to improve lipid profile, but available data still...

Immunoglobulin (Ig) G1 and IgG2a are the surrogate markers respectively for humoral cellular immune responses of hosts against antigens including cystic fluid proteins Cysticercus bovis. A study was conducted to investigate IgG1 Balb/c mice some individual C. bovis in an effort determine roles each protein inducing host.Individual p71, p31, p14 were purified by separation using sodium dodecyl sulfate-polyacrylamide gel electrophoresis elution from gel. Six female immunized 4 times at 10-day...

Background: Inflammation of the synovial membrane involved in knee osteoarthritis (OA) pathomechanism. Cartilage oligomeric matrix protein (COMP) is a cartilage degradation product that has been considered as biomarker OA. Interleukin-6 (IL-6) an inflammatory mediator pathogenesis inflammation this study was conducted to proof whether high level serum COMP and plasma IL-6 increase risk OA. Methods: This case control about primary OA patients who were exposed by circulating IL-6. Every...

BACKGROUND: Preeclampsia (PE) is the disease of theories and second leading cause maternal perinatal morbidities mortalities worldwide. These pathological disturbances will induce inflammation process, oxidative stress, poor subsequent growth on fetus including 32% intrauterine restriction, 22% prematurity, 24% neonatal sepsis asphyxia. There are many about mechanism PE. In molecularly level, it suspected that low level calcitriol interleukin (IL)-10 expressions high expression hypoxia-...