A5011566650- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Atherosclerosis and Cardiovascular Diseases
- Monoclonal and Polyclonal Antibodies Research
- Vasculitis and related conditions
- Cell Adhesion Molecules Research
- Systemic Lupus Erythematosus Research
- Liver Disease Diagnosis and Treatment
- Hepatitis Viruses Studies and Epidemiology
- HIV/AIDS Research and Interventions
- Influenza Virus Research Studies
- Liver Diseases and Immunity
- CAR-T cell therapy research
- Immune Response and Inflammation
- HIV Research and Treatment
- Diabetes and associated disorders
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Otitis Media and Relapsing Polychondritis
- Cancer Immunotherapy and Biomarkers
- Nuclear Receptors and Signaling
- Medical Device Sterilization and Disinfection
- Safe Handling of Antineoplastic Drugs
Sapienza University of Rome
2008-2025
Policlinico Umberto I
2001-2022
Istituto Pasteur
2002-2015
Northwest Medical Specialties
1995-2012
Fondazione Andrea Cesalpino
1997-2008
The San Raffaele Telethon Institute for Gene Therapy
2005
Vita-Salute San Raffaele University
2005
University of Milan
2005
Fondazione Humanitas per la Ricerca
2005
Mario Negri Institute for Pharmacological Research
2005
Regulatory T (TR) cells consist of phenotypically and functionally distinct CD4+ CD8+ cell subsets engaged both in maintaining self-tolerance preventing anti–non-self effector responses (microbial, tumor, transplant, so on) that may be harmful to the host. Here we propose proinflammatory function virus-specific memory CCR7–CD8+ cells, which are massively recruited liver, inefficient (in terms IFN-γ production) patients with chronic hepatitis C virus (HCV) infection because concomitant...
The presentation of exogenous protein antigens in a major histocompatibility complex class I–restricted fashion to CD8+ T cells is called cross-presentation. We demonstrate that cross-presentation soluble viral (derived from hepatitis C virus [HCV], B [HBV], or human immunodeficiency virus) specific cell clones dramatically improved when antigen-presenting dendritic (DCs) are pulsed with the antigen presence chloroquine ammonium chloride, which reduce acidification endocytic system. export...
Abstract CTL responses against multiple hepatitis C virus (HCV) epitopes were detected in 7 of 29 (24.1%) healthy family members (HFM) persistently exposed to chronically HCV-infected patients (HCV-HFM). These precursor at very low or undetectable frequencies, as determined by limiting dilution analysis. However, when HCV-specific effector CD8+ T cells, freshly isolated from PBMC HCV-HFM, assessed a sensitive enzyme-linked immunospot assay, their frequencies severalfold higher than those...
Abstract Dendritic cells (DC) generated after a short‐term exposure of monocytes to IFN‐α and GM‐CSF (IFN‐DC) are highly effective in inducing cross‐priming CD8 + T against viral antigens. We have investigated the mechanisms responsible for special attitude these DC compared their activity with that reference DC. Antigen uptake endosomal processing capabilities were similar IFN‐DC IL‐4‐derived Both types efficiently cross‐presented soluble HCV NS3 protein specific cell clone, even though...
The T-cell-mediated immune response plays a crucial role in defense against hepatotropic viruses as well the pathogenesis of viral chronic hepatitides. However, very little is known about specific T cells during hepatitis delta virus (HDV) infection humans. In this study, T-cell to HDV B (HBV) carriers with superinfection was investigated at different levels. Analysis peripheral blood mononuclear cell (PBMC) proliferation recombinant form large antigen (HDAg) revealed that 8 30 patients...
Abstract Hallmark of acute hepatitis C virus (HCV) infection is a severe virus‐specific effector CD8 + T cell dysfunction that seems to be critical factor in preventing the resolution and favoring onset chronic liver immunopathology. We suggest this establishment HCV persistence, unless it compensated by multispecific responses, as found individuals resolving infection. Analyses on purified populations indicate central memory HCV‐specific CCR7 /CD8 cells efficiently proliferate differentiate...
In our study, we investigated the role of CD39 on tumor-infiltrating CD8+ T lymphocytes (CD8+ TILs) in colorectal, head and neck pancreatic cancers. Partially confirming recent observations correlating expression with T-cell exhaustion, demonstrated a divergent functional activity CD39+ TILs. On one hand, TILs (as compared to their CD39- counterparts) produced significantly lower IFN-γ IL-2 amounts, expressed higher PD-1, inversely correlated perforin granzyme B expression. other, they...
Hemophagocytic lymphohistiocytosis (HLH) is a life-threatening condition characterized by the uncontrolled activation of cytotoxic T lymphocytes, NK cells, and macrophages, resulting in an overproduction pro-inflammatory cytokines. A primary secondary form are distinguished depending on whether or not it associated with hematologic, infectious, immune-mediated disease. Clinical manifestations include fever, splenomegaly, neurological changes, coagulopathy, hepatic dysfunction, cytopenia,...
Background Perinatal HIV-1 infection is acquired in the milieu of a developing immune system, leading to high levels uncontrolled viral replication. Few data have been reported that address dynamics and immunological response infants who initiated aggressive antiretroviral therapy (ART) shortly after birth. Methods Six HIV-1-infected started ART within 3 months age were studied. The median follow-up was 61 months. Plasma RNA, cell-associated DNA, unspliced multiply spliced mRNAs, antibodies,...
Abstract In a companion study, we showed dichotomy between the expansion of central memory (CCR7 + ) hepatitis C virus (HCV)‐specific CTL and incomplete effector differentiation in patients with acute HCV infection. Indeed, cells were unable to perform immediate functions, despite expressing tissue‐homing phenotype – ; semi‐effectors). However, since they promptly differentiated into full‐effectors upon IL‐2 contact, suggested that inhibitory effect by environmental (possibly viral) factors...
Caspase-dependent cleavage of antigens associated with apoptotic cells plays a prominent role in the generation CD8+ T cell responses various infectious diseases. We found that emergence large population autoreactive effector specific for cell-associated self-epitopes exceeds antiviral patients acute hepatitis C virus infection. Importantly, they endow mixed polyfunctional type-1, type-2 and type-17 correlate chronic progression This evolution is related to selection higher receptor avidity,...
According to the immunogenic cell death hypothesis, clinical chemotherapy treatments may result in CD8+ and CD4+ T-cell responses against tumor cells. To discover chemotherapy-associated antigens (CAAs), T cells derived from ovarian cancer (OC) patients (who had been treated with appropriate protocols) were interrogated proteins isolated primary OC We screened for immunogenicity using two-dimensional electrophoresis gel-eluted proteins. Only selected molecularly characterized by...