A5063025236- Monoclonal and Polyclonal Antibodies Research
- Botulinum Toxin and Related Neurological Disorders
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Prostate Cancer Treatment and Research
- Cancer Immunotherapy and Biomarkers
- Advanced Biosensing Techniques and Applications
- Immunotherapy and Immune Responses
- Biochemical and Structural Characterization
- Genetic Neurodegenerative Diseases
- Neurological disorders and treatments
- Muscle Physiology and Disorders
- Atherosclerosis and Cardiovascular Diseases
- Cancer Treatment and Pharmacology
- Intellectual Capital and Performance Analysis
- Prostate Cancer Diagnosis and Treatment
- Chemokine receptors and signaling
- Bacteriophages and microbial interactions
- Blood groups and transfusion
- Cell Adhesion Molecules Research
- Hereditary Neurological Disorders
- Streptococcal Infections and Treatments
- Inflammatory Biomarkers in Disease Prognosis
- Epigenetics and DNA Methylation
- Nanofabrication and Lithography Techniques
Martin University Hospital
2023
Protagen (Germany)
2016
University of Graz
2008
San Francisco General Hospital
1998-2002
University of California, San Francisco
1997-2002
United States Army Medical Research Institute of Infectious Diseases
2002
Wadsworth Center
2001
Boston Biomedical Research Institute
2001
New York State Department of Health
2001
Massachusetts Institute of Technology
2001
A large library of phage-displayed human single-chain Fv antibodies (scFv), containing 6.7 × 10 9 members, was generated by improving the steps construction. Fourteen different protein antigens were used to affinity select from this library. panel specific isolated with each antigen, and contained an average 8.7 scFv. Measurements antibody–antigen interactions revealed several affinities below 1 nM, comparable observed during secondary murine immune response. In particular, four scFv...
The botulinum neurotoxins (BoNTs) cause the paralytic human disease botulism and are one of highest-risk threat agents for bioterrorism. To generate a pharmaceutical to prevent or treat botulism, monoclonal antibodies (mAbs) were generated by phage display evaluated neutralization BoNT serotype A (BoNT/A) in vivo . Although no single mAb significantly neutralized toxin, combination three mAbs (oligoclonal Ab) 450,000 50% lethal doses BoNT/A, potency 90 times greater than hyperimmune...
This investigation was pursued to test the use of intracellular antibodies (intrabodies) as a means blocking pathogenesis Huntington's disease (HD). HD is characterized by abnormally elongated polyglutamine near N terminus huntingtin protein, which induces pathological protein–protein interactions and aggregate formation or its exon 1-containing fragments. Selection from large human phage display library yielded single-chain Fv (sFv) antibody specific for 17 N-terminal residues huntingtin,...
To produce antibodies capable of neutralizing botulinum neurotoxin type A (BoNT/A), the murine humoral immune response to BoNT/A binding domain (H(C)) was characterized at molecular level by using phage antibody libraries. Mice were immunized with H(C), spleens harvested, and single-chain Fv (scFv) libraries constructed from immunoglobulin heavy light chain variable region genes. Phage expressing scFv isolated selection on immobilized H(C). Twenty-eight unique H(C) identified enzyme-linked...
Abstract Background: Several studies have demonstrated the value of DNA methylation in urine-based assays for prostate cancer diagnosis. However, a multicenter validation with clinical prototype has not been published. Methods: We developed multiplexed, quantitative methylation-specific polymerase chain reaction (MSP) assay consisting 3 markers, GSTP1, RARB, and APC, an endogenous control, ACTB, closed-tube, homogeneous format. tested this format urine samples collected after digital rectal...
The domain organization of the botulinum neurotoxin serotype A was studied by using antibody mapping 44 monoclonal single-chain variable fragments. analysis carried out on (i) individual domains holotoxin (binding, translocation, and catalytic), (ii) holotoxin, (iii) in complex with nontoxic portion, (iv) portion recombined vitro. All antibodies mapped to neurotoxin. Forty fragments bound relative isolated domains, suggesting that 4 epitopes are covered when form. Only 20 showed a positive...
Abstract BACKGROUND The currently used prostate cancer serum marker has a low specificity and improved diagnostics are needed. Here we evaluated whether autoantibodies present in sera of patients they useful diagnostic markers for cancer. METHODS Sera from 20 healthy controls were incubated on expression clone arrays containing more than 37,000 recombinant human proteins. Functional annotation clustering the identified autoantigens was performed using DAVID database. Autoantigens group...
Background Chronic inflammation is frequently observed on histological analysis of malignant and non-malignant prostate specimens. It a suspected supporting factor for diseases their progression main cause false positive PSA tests in cancer screening. We hypothesized that induces autoantibodies, which may be useful biomarkers. aimed to identify validate associated serum autoantibodies patients evaluate the expression corresponding autoantigens. Methods Radical prostatectomy specimens (N =...
The biotechnological generation of high affinity monoclonal antibodies (MAbs) has traditionally involved the production hybridomas from spleen cells immunized animals (). This event, together with availability increasingly sophisticated molecular biology and protein engineering techniques, opened up field numerous applications benefits in not only medical but also industrial world. Now use phage offers a new route for antibodies, including human origin, which cannot be easily obtained by...
We describe a novel procedure for determination and validation of off-target activities anti-cluster designation antigen identity 44 (CD44) variant 6 recombinant antibodies by combining two complementary technology platforms; namely UNIchip AV-400, containing printed serial dilution CD44 approximately 400 different human proteins, TISSOMICS, enabling tissue microarray analysis in high-throughput mode. have analyzed the performance monoclonal directed against protein, BMS 116 125, blinded...
Current literature describes many different types of benefits obtained from biobanking operations.However, whether these will actually be realized depends on the large extent various external and internal factors.The main goal this study is to perform an impact analysis emerging program funded by EU grants (European Regional Development Fund), perspective physicians researchers, also identify factors that could influence achievement benefits.First, we briefly describe concept "BIOFORD"...
Abstract Background: Chronic prostatic inflammation of the prostate is one main reasons for false positive serum PSA tests in cancer screening. In addition there accumulating evidence that an inflammatory microenvironment may support development malignancy and progression to metastatic disease. Easy accessible non-invasive indicators would be very helpful a biopsy decision after elevated test result. However, at present, histological examination biopsies remains only way identification...