A5004008884- Cardiac Ischemia and Reperfusion
- Cardiac Arrest and Resuscitation
- Cardiac electrophysiology and arrhythmias
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Genetic and phenotypic traits in livestock
- Anesthesia and Neurotoxicity Research
- Reproductive Physiology in Livestock
- Adenosine and Purinergic Signaling
- Caveolin-1 and cellular processes
- Mitochondrial Function and Pathology
- Cardiac Fibrosis and Remodeling
- Stress Responses and Cortisol
- Mechanical Circulatory Support Devices
- Ruminant Nutrition and Digestive Physiology
- Cardiovascular Effects of Exercise
- Cardiac Imaging and Diagnostics
- Nitric Oxide and Endothelin Effects
- Ion Transport and Channel Regulation
- Effects of Environmental Stressors on Livestock
- Pharmacological Receptor Mechanisms and Effects
- Tryptophan and brain disorders
- Transplantation: Methods and Outcomes
- Receptor Mechanisms and Signaling
- Organ Transplantation Techniques and Outcomes
Griffith University
2015-2024
National Heart Foundation of Australia
2008-2021
Prince Charles Hospital
2019-2020
VA San Diego Healthcare System
2019
University of California, San Diego
2019
The University of Queensland
2019
Heart Foundation
2006-2012
Medical College of Wisconsin
2002-2007
United States Military Academy
2006
Consiglio per la ricerca in agricoltura e l’analisi dell’economia agraria
1965-1972
The aged brain exhibits a loss in gray matter and decrease spines synaptic densities that may represent sequela for neurodegenerative diseases such as Alzheimer's. Membrane/lipid rafts (MLR), discrete regions of the plasmalemma enriched cholesterol, glycosphingolipids, sphingomyelin, are essential development stabilization synapses. Caveolin-1 (Cav-1), cholesterol binding protein organizes signaling components within MLR. It is unknown whether synapses dependent on an age-related Cav-1...
Summary Three techniques, lamb-suckling and test-weighing, hand- or machine-milking following administration of oxytocin, measurements body-water turnover, used for the estimation milk yield in suckled sheep are discussed relation to sources error comparability estimates. Results presented from an experiment which oxytocin techniques were compared. The mean daily production Scottish Blackface East Friesland × ewes suckling single twin lambs was measured by both methods at weekly intervals 6...
Background: Ischemia–reperfusion injury (IRI) is one of the major risk factors implicated in morbidity and mortality associated with cardiovascular disease. During cardiac ischemia, buildup acidic metabolites results decreased intracellular extracellular pH, which can reach as low 6.0 to 6.5. The resulting tissue acidosis exacerbates ischemic significantly affects function. Methods: We used genetic pharmacologic methods investigate role acid-sensing ion channel 1a (ASIC1a) IRI at cellular...
G-protein-coupled receptors (GPCRs) are key mediators in cardiovascular physiology, yet frontline therapies for heart disease target only a small fraction of the cardiac GPCR repertoire. Moreover, there is emerging evidence that GPCRs implicated taste (Tas1r and Tas2rs) have specific functions beyond oral cavity. Our recent description these tissue foreshadows potential novel role cells. In this study, we identified agonist ligands cardiac-Tas2rs to enable functional investigation tissue....
1. Using blood plasma FFA and ketone values to assess the nutritional state of individual ewes, high (H), medium (M) low (L) planes nutrition were imposed on 3 groups Blackface ewes during pregnancy. By this method, bearing single or twin foetuses equally well severely undernourished within treatment groups. The effects these pregnancy have been assessed in terms milk production, lamb growth, live-weight changes by duriug their subsequent lactation. Immediately after parturition all...
Abstract We critically review potential involvement of trimethylamine N -oxide (TMAO) as a link between diet, the gut microbiota and CVD. Generated primarily from dietary choline carnitine by bacteria hepatic flavin-containing mono-oxygenase (FMO) activity, TMAO could promote cardiometabolic disease when chronically elevated. However, control circulating is poorly understood, age, body mass, sex hormones, renal clearance, FMO3 expression genetic background may explain little 25 % variance....
We used pharmacological agents and genetic methods to determine whether the potent A<sub>3</sub> adenosine receptor (AR) agonist 2-chloro-<i>N</i><sup>6</sup>-(3-iodobenzyl)adenosine-5′-<i>N</i>-methylcarboxamide (Cl-IB-MECA) protects against myocardial ischemia/reperfusion injury in mice via A<sub>3</sub>AR or interactions with other AR subtypes. Pretreating wild-type (WT) Cl-IB-MECA reduced infarct size induced by 30 min of coronary occlusion 24 h reperfusion at doses (30 100 μg/kg) that...
The Langendorff mouse heart model is widely employed in studies of myocardial function and responses to injury (e.g. ischaemia). Nonetheless, marked variability exists its preparation functional properties. We examined the impact early growth (8, 16, 20 24 weeks), sex, perfusion fluid [Ca 2+ ] pacing rate on contractile min ischaemia followed by 45 reperfusion. also assessed strain, tested utility studying coronary function. Under normoxic conditions, hearts from 8‐week‐old male C57BL/6 mice...
The relative roles of free-radical production, mitochondrial ATP-sensitive K + (mitoK ATP ) channels and possible receptor cross-talk in both opioid adenosine A 1 (A AR) mediated protection were assessed a rat model myocardial infarction. Sprague-Dawley rats subjected to 30 min occlusion 90 reperfusion. untreated exhibited an infarct 58.8 ± 2.9% [infarct size (IS)/area at risk (AAR), %] the end Pretreatment with either nonselective agonist morphine or selective AR...
The genesis of the ischaemia intolerant phenotype in aged myocardium is poorly understood. We tested hypothesis that impaired adenosine-mediated protection contributes to ischaemic intolerance, and examined whether this countered by A1 adenosine receptor (A1AR) overexpression. Responses 20 min 45 reperfusion were assessed perfused hearts from young (2-4 months) moderately (16-18 mice. Post-ischaemic contractility was ageing with elevated ventricular diastolic (32 +/- 2 vs. 18 mmHg young)...
A 5-day exposure to morphine exerts a profound cardioprotective phenotype in murine hearts. In the present study, we examined mechanisms by which generates this effect, exploring roles of G i and s proteins, PKA, PKC, β-adrenergic receptors (β-AR) acute chronic opioid preconditioning. Langendorff-perfused hearts from placebo, (AM; 10 μmol/l)-, or (CM)-treated mice (75-mg pellet, 5 days) underwent 25-min ischemia 45-min reperfusion. After reperfusion, placebo-treated exhibited marked...
SUMMARY In each of two years, 36 Blackface ewes were brought to a high state body condition at mating and in early gostation. At approximately 16 weeks prepartum the divided into similar groups fed maintain live weight one group (fat), decrease that other (lean). 8 mean differences 14kg scores had been created between groups. During last 6 pregnancy food was rationed all per unit provide theoretical requirements late pregnancy. Both made live-weight gains blood plasma F.F.A. values confirmed...
Hematopoietic stem cells surrender organelles during differentiation, leaving mature red blood (RBC) devoid of transcriptional machinery and mitochondria. The resultant absence cellular repair capacity limits RBC circulatory longevity, old are removed from circulation. specific age-dependent alterations required for this apparently targeted removal RBC, however, remain elusive. Here, we assessed the function Piezo1, a stretch-activated transmembrane cation channel, within subpopulations...