A5089791620- Topic Modeling
- Plant biochemistry and biosynthesis
- Natural Language Processing Techniques
- Information Retrieval and Search Behavior
- Monoclonal and Polyclonal Antibodies Research
- Plant Stress Responses and Tolerance
- RNA and protein synthesis mechanisms
- Plant tissue culture and regeneration
- Text and Document Classification Technologies
- Cardiac Ischemia and Reperfusion
- HER2/EGFR in Cancer Research
- Microbial Metabolic Engineering and Bioproduction
- Plant Gene Expression Analysis
- CAR-T cell therapy research
- Computational Drug Discovery Methods
- Cardiac Arrest and Resuscitation
- Protein Structure and Dynamics
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Microbial Natural Products and Biosynthesis
- Phytochemistry and Biological Activities
- Fungal Biology and Applications
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Web Data Mining and Analysis
Northwest A&F University
2025
First Hospital of Shanxi Medical University
2024
Henan University of Traditional Chinese Medicine
2016-2024
First Affiliated Hospital of Henan University
2018-2024
Shanxi Medical University
2024
Iowa State University
2013-2023
Anhui Agricultural University
2021-2022
Qingdao Agricultural University
2021
Anhui University
2013-2021
Shandong University
2017-2021
Abstract Background The terpenoid indole alkaloid (TIA) pathway leads to the production of pharmaceutically important drugs, such as anticancer compounds vinblastine and vincristine. Unfortunately, these drugs are produced in trace amounts, causing them be very costly. To increase an improved understanding TIA regulatory is needed. Towards this end, transgenic Catharanthus roseus hairy roots that overexpress ORCA2 transcriptional activator were generated characterized. Results...
The intestinal microbiota is significantly affected by the external environment, but our understanding of effects extreme environments such as plateaus far from adequate. In this study, we systematically analyzed variation in and 76 blood clinical indexes among 393 healthy adults with different plateau living durations (Han individuals no living, for 4 to 6 days, >3 months, who returned plain 3 well plateau-living Tibetans). results showed that high-altitude environment rapidly (4 days)...
Immune checkpoint blockade has shown significant clinical benefit in multiple cancer indications, but many patients are either refractory or become resistant to the treatment over time. HER2/neu oncogene overexpressed invasive breast associates with more aggressive diseases and poor prognosis. Anti-HER2 mAbs, such as trastuzumab, currently standard of care for HER2-overexpressing cancers, response rates below 30% generally suffer relapse within a year. In this study we developed bispecific...
The probability that a term appears in relevant documents (P(t | R)) is fundamental quantity several probabilistic retrieval models, however it difficult to estimate without relevance judgments or model. We call this value necessity because measures the percentage of retrieved by - how necessary term's occurrence document relevance. Prior research typically either set constant, estimated based on inverse frequency, neither which was very effective.
The oncoprotein MDM2 negatively regulates the activity and stability of p53 tumor suppressor is an important molecular target for anticancer therapy. Aided by mirror image phage display native chemical ligation, we have previously discovered several proteolysis-resistant duodecimal d-peptide antagonists MDM2, termed (D)PMI-α, β, γ. prototypic inhibitor (D)PMI-α binds ((25-109))MDM2 at affinity 220 nM kills cells in vitro inhibits growth vivo reactivating pathway. Herein, report design a...
Abnormal DNA methylation/demethylation is recognized as a hallmark of cancer. TET (ten-eleven translocation) family members are novel demethylation related proteins that dysregulate in multiple malignances. However, their effects on ovarian cancer remain to be elucidated. The changes during TGF-β1-induced epithelial-mesenchymal transition (EMT) SKOV3 and 3AO cells were detected. TET3 was ectopically expressed TGF-β1-treated examine its effect EMT phenotype. downstream target further...
Pancreatic cancer is the fourth leading cause of cancer-related deaths worldwide. However, therapeutic strategies for treatment pancreatic are still limited. Therefore, it urgent us to develop novel effective therapies cancer. In this study, we explored effects rosmarinic acid on progression and underlying molecular mechanisms. Rosmarinic significantly suppressed cell viability, growth, invasion migration as well epithelial mesenchymal transition (EMT) cells, induced apoptosis in cells....
Yarrowia lipolytica is an oleaginous yeast that recognized for its ability to accumulate high levels of lipids, which can serve as precursors biobased fuels and chemicals. Polyketides, such triacetic acid lactone (TAL), also a precursor diverse commodity This study used Y. host organism the production TAL via expression 2-pyrone synthase gene from Gerbera hybrida. Induction lipid biosynthesis by nitrogen-limited growth conditions increased titers. We manipulated basal using DNA cut-and-paste...
The majority of patients with metastatic breast cancer who are treated the anti-HER2 monoclonal antibody, trastuzumab, generally develop resistance to drug within a year after initiation treatment. Here we describe new humanized 19H6-Hu, which binds HER2 extracellular domain (ECD) high affinity and inhibits proliferation multiple HER2-overexpressing cell lines as single agent or in combination trastuzumab. 19H6-Hu III proximity IV ECD, differs from trastuzumab pertuzumab. was more effective...
Diterpenoids play important roles in rice microbial disease resistance as phytoalexins, well acting allelopathy and abiotic stress responses. Recently, the casbane-type phytoalexin ent-10-oxodepressin was identified rice, but its biosynthesis has not yet been elucidated. Here investigated via co-expression analysis biochemical characterisation, with use of CRISPR/Cas9 technology for genetic analysis. The results a biosynthetic gene cluster (BGC) on chromosome 7 (c7BGC), containing relevant...
Silver nanoparticles (AgNPs), widely recognized for their nanoscale geometric size and unique properties, such as large specific surface area, high permeability, safety, were synthesized using the endophytic fungus
The green alga Chlamydomonas reinhardtii is a model organism to study H(2) metabolism in photosynthetic eukaryotes. To understand the molecular mechanism of metabolism, we used 2-DE coupled with MALDI-TOF and MALDI-TOF/TOF-MS investigate proteomic changes cells that undergo sulfur-depleted photoproduction process. In this report, obtained 2-D PAGE soluble protein profiles at three time points representing different phases leading production. We found over 105 Coomassie-stained spots,...
The E3 ubiquitin ligase MDM2 functions as a crucial negative regulator of the p53 tumor suppressor protein by antagonizing transactivation activity and targeting for degradation. Cellular stress activates alleviating MDM2-mediated functional inhibition, even though molecular mechanisms stress-induced activation still remain poorly understood. Two opposing models have been proposed to describe structural role in Ser17 phosphorylation N-terminal “lid” (residues 1–24) MDM2. Using native...
The substrate specificity of acyl-ACP thioesterase (TE) plays an essential role in controlling the fatty acid profile produced by type II synthases. Here we identify two groups residues that synergistically determine different specificities TEs from Cuphea viscosissima (CvFatB1 and CvFatB2). One group (V194, V217, N223, R226, R227, I268 CvFatB2) is critical determining structure depth a hydrophobic cavity N-terminal hotdog domain binds substrate's acyl moiety. other (255-RKLSKI-260...
Abstract DNA-binding hot spot residues of proteins are dominant and fundamental interface that contribute most the binding free energy protein–DNA interfaces. As experimental methods for identifying spots expensive time consuming, computational approaches urgently required in predicting on a large scale. In this work, we systematically assessed wide variety 114 features from combination protein sequence, structure, network solvent accessible information their combinations along with various...
Terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus is a complex and highly regulated process. Understanding the biochemistry regulation of TIA pathway particular interest as it may allow engineering plants to accumulate higher levels pharmaceutically important alkaloids. Toward this end, we generated transgenic C. hairy root line that overexpresses CrBPF1 transcriptional activator under control β-estradiol inducible promoter. MYB-like protein was previously postulated help...