A5064676997- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Neural dynamics and brain function
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Neurogenesis and neuroplasticity mechanisms
- Neurotransmitter Receptor Influence on Behavior
- Biochemical effects in animals
- Biochemical Analysis and Sensing Techniques
- Neuroscience of respiration and sleep
- Photoreceptor and optogenetics research
- CAR-T cell therapy research
- Autism Spectrum Disorder Research
- Light effects on plants
- Nerve injury and regeneration
- Retinal Development and Disorders
- Cholinesterase and Neurodegenerative Diseases
- CRISPR and Genetic Engineering
- Genetics, Aging, and Longevity in Model Organisms
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Memory Processes and Influences
Tufts University
2011-2020
Scripps Research Institute
2006-2009
Utrecht University
1999-2001
University Medical Center Utrecht
2000-2001
Do learning and retrieval of a memory activate the same neurons? Does number reactivated neurons correlate with strength? We developed transgenic mouse that enables long-lasting genetic tagging c-fos-active neurons. found in basolateral amygdala are activated during Pavlovian fear conditioning retrieval. The correlated positively behavioral expression memory, indicating stable neural associative memory. ability to manipulate these genetically should allow more precise dissection molecular...
The stabilization of long-term memories requires de novo protein synthesis. How can proteins, synthesized in the soma, act on specific synapses that participate a given memory? We studied dynamics newly AMPA-type glutamate receptors (AMPARs) induced with learning using transgenic mice expressing GluR1 subunit fused to green fluorescent (GFP-GluR1) under control c-fos promoter. found learning-associated recruitment GFP-GluR1 selectively mushroom-type spines adult hippocampal CA1 neurons 24...
This study describes, for the first time, rhythmic translational program within circadian clock cells. The results indicate that most cell mRNAs are translated at low-energy times of either mid-day or mid-night, and also related cellular functions coordinately regulated by synchronized translation relevant same time day.
Abstract Learned fear and safety are associated with distinct oscillatory states in the basolateral amygdala (BLA) medial prefrontal cortex (mPFC). To determine if how these network support retrieval of competing memories, we mimicked endogenous activity through optogenetic stimulation parvalbumin-expressing interneurons mice during contextual extinction memories. We found that exogenously induced 4 Hz 8 BLA exerts bi-directional control over conditioned freezing behavior an experience-...
It is currently thought that memory formation requires the activation of NMDA receptors (NMDARs) in hippocampus. However, recent studies indicate these are not necessary for all forms learning. The current experiments examine this issue using context fear conditioning mice. First, we show can be acquired without NMDAR previously trained animals. Mice one environment (context A) subsequently able to learn about a second B) presence antagonists. Second, demonstrate NMDAR-independent learning...
The use of molecular tools to study the neurobiology complex behaviors has been hampered by an inability target desired changes relevant groups neurons. Specific memories and specific sensory representations are sparsely encoded a small fraction neurons embedded in sea morphologically functionally similar cells. In this review we discuss genetics techniques that being developed address difficulty. several studies promoter elements responsive neural activity have used drive long-lasting...
Memory loss is one of the hallmark symptoms Alzheimer's disease (AD). It has been proposed that soluble amyloid-beta (Abeta) oligomers acutely impair neuronal function and thereby memory. We here report natural Abeta contextual fear memory in mice. A oligomer solution containing monomers, dimers, trimers, tetramers was derived from conditioned medium 7PA2 cells, a cell line expresses human amyloid precursor protein Val717Phe familial AD mutation. As control we used which were removed through...
Autism spectrum disorder (ASD) is a highly prevalent and genetically heterogeneous brain disorder. Developing effective therapeutic interventions requires knowledge of the regions that malfunction how they during ASD-relevant behaviors. Our study provides insights into activated by novel social stimulus activation pattern differs between mice display autism-like disabilities control littermates. Adenomatous polyposis coli (APC) conditional knockout (cKO) reduced interest, increased...
Targeted mutagenesis in mice has shown that genes from a wide variety of gene families are involved memory formation. The efficient identification learning and could be achieved by random combined with high-throughput phenotyping. Here, we provide the first report screen generated mutants mouse. We tested group N-ethyl-N-nitrosourea (ENU) mutagenized conditioned fear paradigm. screened for both dominant recessive mutations caused impairments contextual or tone conditioning. Heritability...
Acquiring the gene expression profiles of specific neuronal cell-types is important for understanding their molecular identities. Genome-wide genetically defined can be acquired by collecting and sequencing mRNA that bound to epitope-tagged ribosomes (TRAP; translating ribosome affinity purification). Here, we introduce a transgenic mouse model combines TRAP technique with tetracycline transactivator (tTA) system expressing EGFP-tagged ribosomal protein L10a (EGFP-L10a) under control...
The brain contains a large variety of projection neurons with different functional properties. properties arise from their connectivity other and molecular composition. We describe novel tool for obtaining the gene expression profiles that are anatomically defined by location soma axon terminals. Our utilizes adeno-associated virus serotype 9 (AAV9), which we found to retrogradely transduce after injection at site used AAV9 express Enhanced Green Fluorescent Protein (EGFP)-tagged ribosomal...
Abstract Both the basal amygdala (BA) and bed nucleus of stria terminalis (BNST) can participate in contextual fear, but it is unclear whether fear engrams involve a direct interaction between these two brain regions. To determine dorsal BNST (dBNST)-projecting neurons BA engrams, we combined TetTag mouse with retrograde tracer to label dBNST-projecting cells BA. We identified population located anterior subdivision (aBA) that was activated during conditioning reactivated retrieval did not...