A5025829442- Immune cells in cancer
- Immune Response and Inflammation
- Cancer-related molecular mechanisms research
- Genetic and phenotypic traits in livestock
- Veterinary Equine Medical Research
- Immune Cell Function and Interaction
- Enhanced Recovery After Surgery
- Neuroinflammation and Neurodegeneration Mechanisms
- Reproductive Physiology in Livestock
- Clinical Nutrition and Gastroenterology
- Cytokine Signaling Pathways and Interactions
- Molecular Biology Techniques and Applications
- Chemokine receptors and signaling
- Phagocytosis and Immune Regulation
- Livestock Farming and Management
- Intensive Care Unit Cognitive Disorders
- Bladder and Urothelial Cancer Treatments
- Diet and metabolism studies
- Radiation Dose and Imaging
- Platelet Disorders and Treatments
- Gastrointestinal motility and disorders
- Tissue Engineering and Regenerative Medicine
- Cancer-related gene regulation
- Reproductive System and Pregnancy
- Autopsy Techniques and Outcomes
Roslin Institute
2016-2021
University of Edinburgh
2016-2021
Bell Equine Veterinary Clinic
2011-2013
Abstract The proliferation, differentiation and survival of mononuclear phagocytes depend on signals from the receptor for macrophage colony-stimulating factor, CSF1R. mammalian Csf1r locus contains a highly conserved super-enhancer, fms -intronic regulatory element (FIRE). Here we show that genomic deletion FIRE in mice selectively impacts CSF1R expression tissue development specific tissues. Deletion ablates murine embryonic stem cells. ΔFIRE/ΔFIRE lack macrophages embryo, brain microglia...
We have produced Csf1r-deficient rats by homologous recombination in embryonic stem cells. Consistent with the role of Csf1r macrophage differentiation, there was a loss peripheral blood monocytes, microglia brain, epidermal Langerhans cells, splenic marginal zone macrophages, bone-associated macrophages and osteoclasts, peritoneal macrophages. Macrophages red pulp, liver, lung, gut were less affected. The pleiotropic impacts on development multiple organ systems distinct from those reported...
The F4/80 antigen, encoded by the Adgre1 locus, has been widely-used as a monocyte-macrophage marker in mice, but its value macrophage other species is unclear, and even questioned. ADGRE1 seven transmembrane G protein-coupled receptor with an extracellular domain containing repeated Epidermal Growth Factor (EGF)-like calcium binding domains. Using new monoclonal antibody, we demonstrated that myeloid differentiation pigs, absent from progenitors bone marrow, highly-expressed mature...
Abstract CSF1 is the primary growth factor controlling macrophage numbers, but whether expression of receptor differs between discrete populations mononuclear phagocytes remains unclear. We have generated a Csf1r-mApple transgenic fluorescent reporter mouse that, in combination with lineage tracing, Alexa Fluor 647–labeled CSF1-Fc and CSF1, modified ΔCsf1–enhanced cyan protein (ECFP) transgene that lacks 150 bp segment distal promoter, we used to dissect differentiation responsiveness...
Objective Postoperative ileus (POI) is assumed to result from myeloid cells infiltrating the intestinal muscularis externa (ME) in patients undergoing abdominal surgery. In current study, we investigated role of monocytes a murine model manipulation (IM)-induced POI order clarify whether mediate tissue damage and dysfunction or they are rather involved recovery gastrointestinal (GI) motility. Design IM was performed mice with defective monocyte migration tissues (C-C motif chemokine receptor...
Equine mesenchymal stem/stromal cells (MSCs) are multipotent that widely used for treatment of musculoskeletal injuries, and there is significant interest in expanding their application to nonorthopedic conditions. MSCs possess antibacterial immunomodulatory properties may be relevant combating infection; however, comparative studies using from different origins have not been carried out the horse, this was focus study. Our results showed MSC-conditioned media attenuated growth Escherichia...
Macrophage colony-stimulating factor (CSF1) is an essential growth and differentiation for cells of the macrophage lineage. To explore role CSF1 in steady-state control monocyte production tissue repair, we previously developed a bioactive protein with longer half-life circulation by fusing pig Fc region IgG1a. CSF1-Fc administration to pigs expanded progenitor pools marrow selectively increased numbers their expression maturation marker CD163. There was rapid increase size liver, extensive...
Abstract Activated mouse macrophages metabolize arginine via NO synthase (NOS2) to produce as an antimicrobial effector. Published gene expression datasets provide little support for the activation of this pathway in human macrophages. Generation requires coordinated regulation multiple genes. We have generated RNA-sequencing data from bone marrow–derived representative rodent (rat), monogastric (pig and horse), ruminant (sheep, goat, cattle, water buffalo) species, analyzed genes involved...
The domestic water buffalo (Bubalus bubalis) makes a major contribution to the global agricultural economy in form of milk, meat, hides and draught power. population is predominantly found Asia, per head more people depend upon than on any other livestock species. Despite its importance, there are comparatively fewer genomic transcriptomic resources available for We have generated large-scale gene expression atlas covering multiple tissue cell types from all organ systems collected three...
Mammalian macrophages differ in their basal gene expression profiles and response to the toll-like receptor 4 (TLR4) agonist, lipopolysaccharide (LPS). In human macrophages, LPS elicits a temporal cascade of transient including feed forward activators feedback regulators that limit response. Here we present transcriptional network analysis sheep bone marrow-derived (BMDM) based upon RNA-seq at 0, 2, 4, 7, 24 h post-stimulation. The reveals conserved transcription factor with humans, rapid...
Goats (Capra hircus) are an economically important livestock species providing meat and milk across the globe. They of particular importance in tropical agri-systems contributing to sustainable agriculture, alleviation poverty, social cohesion utilisation marginal grazing. There excellent genetic genomic resources available for goats, including a highly contiguous reference genome (ARS1). However, gene expression information is limited comparison other ruminants. To support functional...
Abstract Expression of Csf1r in adults is restricted to cells the macrophage lineage. Transgenic reporters based upon locus require inclusion highly conserved Fms-intronic regulatory element for expression. We have created Csf1r-EGFP transgenic sheep via lentiviral transgenesis a construct containing elements mouse and promoter. Committed bone marrow precursors blood monocytes express EGFP these animals. Sheep were divided into three populations, similar classical, intermediate, nonclassical...
One of the most significant physiological challenges to neonatal and juvenile ruminants is development establishment rumen. Using a subset RNA-Seq data from our high-resolution atlas gene expression in sheep (Ovis aries) we have provided first comprehensive characterization transcription entire gastrointestinal (GI) tract during transition pre-ruminant ruminant. The dataset comprises 164 tissue samples at four different time points (birth, one week, 8 weeks adult). network cluster analysis...
Summary A 12‐year‐old B elgian W armblood gelding was examined for haematuria and dysuria of 24 h duration. Cystoscopy revealed an intraluminal multinodular soft tissue mass originating from the dorsal bladder neck. Histopathological examination biopsies identified transitional cell carcinoma. The surgically debulked via a temporary perineal urethrotomy. horse commenced treatment with oral piroxicam. Follow‐up 18 months post operatively no evidence tumour recurrence. Neoplasia equine is...
Abstract Goats ( Capra hircus ) are an economically important livestock species providing meat and milk across the globe. They of particular importance in tropical agri-systems contributing to sustainable agriculture, alleviation poverty, social cohesion utilisation marginal grazing. There excellent genetic genomic resources available for goats, including a highly contiguous reference genome (ARS1). However, gene expression information is limited comparison other ruminants. To support...
Studies in rodents and humans have demonstrated that intestinal manipulation or surgical trauma initiates an inflammatory response the intestine which results leucocyte recruitment to muscularis externa causing smooth muscle dysfunction.To examine horses undergoing colic surgery by measuring relative differential gene expression tissues harvested from cases control horses.Prospective case-control study.Mucosa were healthy margins of resected small (n = 12) derived euthanised for reasons...
Abstract One of the most significant physiological challenges to neonatal and juvenile ruminants is development establishment rumen. Using a subset RNA-Seq data from our high-resolution atlas gene expression in sheep ( Ovis aries ) we have provided first comprehensive characterisation transcription entire gastrointestinal (GI) tract during transition pre-ruminant ruminant. The dataset comprises 168 tissue samples at four different time points (birth, one week, 8 weeks adult). network cluster...
Searchable abstracts of presentations at key conferences on reproductive biology and medicine ISSN 2052-1472 (online)