A5001080962- Diabetic Foot Ulcer Assessment and Management
- Wound Healing and Treatments
- Genetics and Neurodevelopmental Disorders
- Hepatitis C virus research
- Hepatitis B Virus Studies
- Genomics and Rare Diseases
- Virology and Viral Diseases
- Pressure Ulcer Prevention and Management
- Ocular Disorders and Treatments
- Liver Disease Diagnosis and Treatment
- Bacterial biofilms and quorum sensing
- Genetic Neurodegenerative Diseases
- Autism Spectrum Disorder Research
- Brucella: diagnosis, epidemiology, treatment
- Viral Infections and Vectors
- Oral microbiology and periodontitis research
- Corneal Surgery and Treatments
- Animal Virus Infections Studies
- Skin Protection and Aging
- Biochemical Analysis and Sensing Techniques
- Retinal Development and Disorders
- Animal Disease Management and Epidemiology
- Hereditary Neurological Disorders
- Pneumonia and Respiratory Infections
- melanin and skin pigmentation
Kohat University of Science and Technology
2016-2025
Institute of Biomedical and Genetic Engineering
2016
Pakistan Institute of Medical Sciences
2016
National Institute of Health
2007
Background . Mycobacterium tuberculosis ( M. ) that causes (TB) kills millions of infected people annually especially multidrug-resistant (MDR-TB). On infection, macrophages recognize the mycobacteria by toll-like receptor (TLR) followed phagocytosis and control mycobacteria. In addition, also secrete IL-12 to induce IFN- γ production T, which, in turn, increases oxidative burst. Individuals with defects innate or adaptive immunity exhibit increased susceptibility Understanding these...
Distribution of Hepatitis C Virus (HCV) genotypes may be changed over time. Epidemiological Studies on distribution patterns HCV in Pakistani population might assist for better treatment options and preventive strategies.This study was conducted to determine different geographical regions Pakistan.In this cross-sectional study, 1818 randomly selected patients from Pakistan, diagnosed with infection by the third generation Enzyme Linked Immunosorbent Assay (ELISA), were included between April...
The epidemiological significance of Hepatitis B virus genotypes has been well established and becoming an essential concern day by however, much little is known about the mixed infection with more than one their clinical relevance.Intravenous drug abusers are considered as a major risk group for acquisition transmission blood borne infections like hepatitis B, in Pakistan, no such data ever reported epidemiology HBV its Injecting Drug Users. 250 individuals were analyzed after prior...
Chronic wound infections impose major medical and economic costs on health-care systems, cause significant morbidity, mortality prolonged hospitalisation. The presence of biofilm producing bacteria in these wounds is considered as an important virulence factor that leads to chronic implications including ulceration. undertaken study aimed isolate identify the aerobic bacterial pathogens from patients with infections, determine their antibiotics resistance profiles Method: During this study,...
Histological and hematological disturbance caused by Arsenic containing water were studied in mice model. Animal divided into four groups. Control group exposed to arsenic free distilled 3 treatmental the with 30, 150 300 ppb. Blood samples organs collected after 40 days. Histopathological results revealed mild severe type of necrosis degenerative changes kidney liver feed animals. Kidney ppb showed distal proximal tubules. The renocytes tubules showing hydropic fatty degeneration. Due cells...
Determination of an individual’s hepatitis C virus (HCV) genotypes prior to antiviral therapy has become increasingly important for the clinical management and prognosis HCV infection. Therefore, this study was conducted investigate prevalence in infected patients district Bannu Khyber Pakhtunkhwa region Pakistan. Serum samples 117 seropositive were screened HCV-RNA by using reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) then PCR positive subjected genotyping. Out...
Autosomal recessive anophthalmia and microphthalmia are rare developmental eye defects occurring during early fetal development. Syndromic non-syndromic forms of demonstrate extensive genetic allelic heterogeneity. To date, disease mutations have been identified in 29 causative genes associated with microphthalmia, autosomal dominant, X-linked inheritance patterns described. Biallelic ALDH1A3 gene variants the leading causes countries frequent parental consanguinity. This study describes...
Peste des petits Ruminants (PPR) is an acute, febrile, highly contagious and economically important viral disease of small ruminants.However PPR more prevalent in sheep goat.Competitive ELISA, Virus neutrilization test, RT-PCR are the available techniques for diagnosis which give rapid detection where as Agar gel immunodiffusion Counter immunoelectrophoresis were previously used detection.In this study two serological compared diagnosis.The main aim was to evaluate comparative sensitivity...
Background: Pseudomonas aeruginosa is a Gram-negative and rod-shaped opportunistic pathogen highly involved in biofilm production chronic wounds. Biofilms wounds are the main cause of resistance to multiple antimicrobial agents. This study aimed identify biofilm-producing bacteria most frequently found examine association antibiotic among isolates. Objectives: was evaluate isolation P. from different types wounds, as one causes delay wound healing formation, well determine Methods:...
Objective: Studies on diabetic foot ulcers (DFU) involving a representative sample of patients in Pakistan are scarce. This study aimed to determine baseline characteristics infected DFUs hospitalised at the Institute Medical Sciences Islamabad. Method: In this cross-sectional study, carried out during May 2015 and June 2016, ulcer with were investigated documented. From DFUs, aerobic bacterial pathogens isolated, identified evaluated for antimicrobial susceptibility. Results: A total 214...
Retinitis pigmentosa (RP) is the most common inherited retinal dystrophy, affecting approximately 1 in 4000 individuals worldwide. The form of syndromic RP Usher syndrome (USH) accounting for 20-30 % cases. Mutations USH2A gene cause a significant proportion recessive non-syndromic and USH type II (USH2). This study aimed to determine causative role autosomal ocular diseases establish genotype-phenotype correlation associated with variants.We performed direct Sanger sequencing co-segregation...
Abstract Background Epilepsy, a challenging neurological condition, is often present with comorbidities that significantly impact diagnosis and management. In the Pakistani population, where financial limitations geographical challenges hinder access to advanced diagnostic methods, understanding genetic underpinnings of epilepsy its associated conditions becomes crucial. Methods This study investigated four distinct families, each presenting spectrum comorbidities, using combination whole...
Hereditary motor and sensory neuropathy (HMSN) refers to a group of inherited progressive peripheral neuropathies characterized by reduced nerve conduction velocity with chronic segmental demyelination and/or axonal degeneration. HMSN is highly clinically genetically heterogeneous multiple inheritance patterns phenotypic overlap other neurodegenerative diseases. Due this high complexity genetic heterogeneity, study aimed elucidate the causes in Pakistani families using Whole Exome Sequencing...
To map Usher phenotype in a consanguineous Pakistani family and identify disease-associated mutation causative gene to establish phenotype-genotype correlation.A which was segregating as an autosomal recessive trait ascertained. On the basis of results clinical investigations affected members this disease diagnosed syndrome (USH). locus responsible for family, genomic DNA from blood sample each individual genotyped using microsatellite Short Tandem Repeat (STR) markers known loci. Then...
In the current study, possible outcome of gender difference and genotypic polymorphism ABCB1 gene encoding P-glycoprotein on pharmacokinetics azithromycin has been evaluated. An open-label, comparative pharmacokinetic study was done in healthy Pakistani volunteers (females (n = 8) males 8)). They were administered a single 500 mg oral dose azithromycin. Blood samples (≈5 mL) collected heparinized tubes HPLC/MS/MS method used to determine plasma levels. (single nucleotide polymorphisms) at...
In the following study, comparative efficacy of Milk Ring Test (MRT), Serum Plate Agglutination (SPAT) was determined by calculating sensitivities and specificities for detection Brucellosis in bovines from district Peshawar Khyber PakhtunKhwa, Pakistan.Considering Polymerase Chain Reaction (PCR) as a gold standard diagnostic test, MRT showed very low sensitivity (4.8%), while its specificity 90.9 %, likewise, SPAT also (41 %), 66.7% bovines.Statistically, high recommended poor these tests,...
Abstract Oculocutaneous albinism (OCA) is a genetic disorder of melanin synthesis that results in hypopigmentation hair, skin and eyes. OCA has been reported individuals from all ethnic backgrounds but it more common among those with Europeans ancestry. heterogeneous group disorders seven types are caused by mutations TYR (OCA1), OCA2 (OCA2), TYRP1 (OCA3), SLC45A2 (OCA4), SLC24A5 (OCA6) C10oRF11 (OCA7) genes. However, MC1R gene variants have modify phenotype the knowledge about function...
Abstract Background Intellectual disability (ID) can be associated with different syndromes such as Rubinstein‐Taybi syndrome (RSTS) and also related to conditions metabolic encephalomyopathic crises, recurrent,with rhabdomyolysis, cardiac arrhythmias neurodegeneration. Rare congenital RSTS1 (OMIM 180849) is characterized by mental growth retardation, significant duplicated distal phalanges of thumbs halluces, facial dysmorphisms, an elevated risk malignancies. Microdeletions point mutations...