A5008408495- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Heavy metals in environment
- Reproductive tract infections research
- Calcium signaling and nucleotide metabolism
- Photoreceptor and optogenetics research
- Cellular transport and secretion
- Syphilis Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Gout, Hyperuricemia, Uric Acid
- Photosynthetic Processes and Mechanisms
- Electrochemical sensors and biosensors
- Blood groups and transfusion
- Advanced Nanomaterials in Catalysis
- Enzyme Catalysis and Immobilization
- Lysosomal Storage Disorders Research
- Genomics and Phylogenetic Studies
- Mast cells and histamine
- Plant Stress Responses and Tolerance
- Urinary Tract Infections Management
- Complement system in diseases
- Heavy Metals in Plants
- Trace Elements in Health
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Centogene (Germany)
2022-2023
Forschungszentrum Jülich
2015-2018
University of Konstanz
2008-2012
Abstract The controversial discussion about the role of Chlamydia pneumoniae in atherosclerosis cannot be solved without a reliable diagnosis that allows discrimination between past and persistent infections. Using proteomic approach immunoblotting with human sera, we identified 31 major C. Ags originating from 27 different proteins. More than half proteins represent not described previously. comparative analysis spot reactivity Pmp6, OMP2, GroEL, DnaK, RpoA, EF-Tu, as well CpB0704 CpB0837,...
Ceroid lipofuscinoses neuronal 6 (CLN6) disease belongs to the ceroid (NCLs), complex and genetically heterogeneous disorders with wide geographical phenotypic variation. The first clinical signs usually appear between 18 months 8 years, but examples of later-onset have also been reported. Common manifestations include ataxia, seizures, vision impairment, developmental regression. Because these are shared by other neurological diseases, identification CLN6 genetic variants is imperative for...
The primary visual cortex (area V1) is an extensively studied part of the cerebral with well-characterized connectivity, cellular and molecular architecture functions (for recent reviews see Amunts Zilles, Neuron 88:1086–1107, 2015; Casagrande Xu, Parallel pathways: a comparative perspective. neurosciences, MIT Press, Cambridge, pp 494–506, 2004). In humans, V1 defined by heavily myelinated fibers arriving from radiatio optica that form Gennari stripe in cortical layer IV, which further...
Hereditary angioedema (HAE) with C1-inhibitor deficiency (C1-INH-HAE) is a rare disease caused by low level (type I) or dysfunction II) of the protein subsequent reduction certain complement levels.To develop and test reliability two-tier method based on C1-INH C4 quantitation followed genetic analysis from dried blood spot (DBS) for establishing diagnosis C1-INH-HAE. proteins have been quantified in human plasma using classical immuno-assay DBS newly developed proteolytic liquid...
Structure and dynamics of membrane-bound light-harvesting pigment-protein complexes (LHCs), which collect transmit light energy for photosynthesis thereby play an essential role in the regulation photoprotection, were identified characterized using high-resolution Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). LHCs from photosystem II (LHCII) isolated thylakoid membrane Arabidopsis thaliana leaves after stress treatment sucrose density gradient centrifugation,...
We present here a new approach that enabled the identification of protein from bacterial strain with unknown genomic background using combination inverted PCR degenerate primers derived N-terminal sequences and high resolution peptide mass determination proteolytic digests two-dimensional electrophoretic separation. Proteins sulfate-reducing bacterium Desulfotignum phosphitoxidans specifically induced in presence phosphite were separated by gel electrophoresis as series apparent soluble...
We report here an affinity-proteomics approach that combines 2D-gel electrophoresis and immunoblotting with high performance mass spectrometry to the identification of both full length protein antigens antigenic fragments Chlamydophila pneumoniae (C. pneumoniae). The present affinity-mass effectively utilized resolution FTICR LC-tandem-MS for identification, enabled several new highly C. proteins were not hitherto reported or previously detected only in other Chlamydia species, such as...