A5030422950- Connexins and lens biology
- Exercise and Physiological Responses
- Ion channel regulation and function
- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Thermoregulation and physiological responses
- Muscle and Compartmental Disorders
- Intensive Care Unit Cognitive Disorders
- Dialysis and Renal Disease Management
- Heme Oxygenase-1 and Carbon Monoxide
- Nicotinic Acetylcholine Receptors Study
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Thermal Regulation in Medicine
Universidad de Los Andes, Chile
2025
Pontificia Universidad Católica de Chile
2016-2020
Clínica Las Condes
2017
Sepsis pathogenesis is complex, and effective treatments are limited, leading to high mortality rates between 20% 55%. Early identification of factors contributing sepsis-related muscle dysfunction critical for risk stratification potential therapeutic development. The immune response during sepsis affects skeletal muscles, organ worsening prognosis. In this study, we explore the role connexin hemichannels (Cx HCs) in early changes homeostasis sepsis. Using a cecal ligature puncture...
Although connexins (Cxs) are broadly expressed by cells of mammalian organisms, Cx39 has a very restricted pattern expression and the biophysical properties Cx39-based channels [hemichannels (HCs) gap junction (GJCs)] remain largely unknown. Here, we used HeLa transfected with (HeLa-Cx39 cells) in which intercellular electrical coupling was not detected, indicating absence GJCs. However, functional HCs were found on surface exposed to conditions known increase open probability other Cx...
Glucocorticoids are frequently used as anti-inflammatory and immunosuppressive agents. However, high doses and/or prolonged use induce undesired secondary effects such muscular atrophy. Recently, de novo expression of connexin43 connexin45 hemichannels (Cx43 HCs Cx45 HCs, respectively) has been proposed to play a critical role in the mechanism underlying myofiber atrophy induced by dexamethasone (Dex: synthetic glucocorticoid), but their involvement specific muscle changes promoted Dex...
Skeletal muscles (∼50% of the body weight) are affected during acute and late sepsis represent one associate organ dysfunction. Cell membrane changes have been proposed to result from a channelopathy yet unknown cause associated with mitochondrial dysfunction muscle atrophy. We hypothesize that might be explained at least in part by expression non-selective channels. Here, this possibility was studied characterized mice model evident skeletal atrophy induced cecal ligation puncture (CLP). At...
The mechanism of glucocorticoid-induced atrophy remains unclear. Objectives: to evaluate the involvement oxidative stress, connexin hemichannels (Cx HCs) and mitochondrial dysfunction in dexamethasone (DEX)-induced muscle atrophy. Methods: We used specific Cx43/Cx45 expression deficient wild-type mice treated with DEX or DEX+vitaminE. Atrophy was evaluated by atrogin-1 reactivity (immunofluorescense) cross sectional area (CSA) myofibers. functional state mitochondria measuring oxygen...