Abstract With advances in immunology and cancer biology, there is an unmet need for increasingly sensitive systems to monitor the expression of specific cell markers development new diagnostic therapeutic tools. To address this challenge, a highly labeling method that translates antigen–antibody recognition processes into DNA detection events can be greatly amplified via isothermal rolling circle amplification (RCA) applied. By merging single‐molecule power RCA reactions with microfluidic...

The transition from remifentanil intraoperative anesthesia to postoperative analgesia must be planned carefully due the short duration of action (3-10 min) hydrochloride, a potent, esterase-metabolized mu-opioid agonist. This study compared efficacy and safety regimens using or morphine sulfate for immediate pain relief in patients who had surgery under general with remifentanil/propofol.One hundred fifty received open-label propofol participated this multicenter, double-blind, double-dummy...

We explored the analgesic effect of epidural nifedipine in male Sprague-Dawley rats. By using an implanted catheter, rats were given 35 microL dimethylsulfoxide (DMSO) alone or DMSO containing 2.5, 5, 10, 20 microM nifedipine. Analgesia was measured by tailflick (TF) involving spinal reflexes, and hotplate (HP) requiring intact central nervous system. The latencies recorded up to 120 min after injection. cutoff time noxious stimuli s TF 60 HP prevent tissue damage. technique revealed a...

We tested the hypothesis that remifentanil-nitrous oxide (N2O) anesthesia shortens postoperative emergence and recovery compared with an isoflurane-N2O-fentanyl combination in elderly patients undergoing spinal surgery. A total of 60 (>65 yr old) were randomly assigned to one two groups for maintenance anesthesia. After induction 3.6 ± 1.2 mg/kg IV thiopental endotracheal intubation facilitated 1.4 0.5 succinylcholine, maintained either 0.5%–1.5% isoflurane, 70% N2O, up 7 μg/kg fentanyl...

This study was designed to define the appropriate dose of remifentanil hydrochloride alone or combined with midazolam provide satisfactory comfort and maintain adequate respiration for a monitored anesthesia care setting.One hundred fifty-nine patients scheduled outpatient surgery participated in this multicenter, double-blind study. Patients were randomly assigned one two groups: remifentanil, 1 microgram/kg, given over 30 s followed by continuous infusion 0.1 microgram.kg-1.min-1...

Propofol is a commonly used intravenous anesthetic agent, which produce rapid induction of and recovery from general anesthesia. Numerous clinical studies reported that propofol can potentially cause amnesia memory loss in human subjects. The underlying mechanism for this unclear but may be related to the memory-associated genes such as c-Fos Egr-1 by propofol. This study explored effects on expression rat hippocampal slices.Hippocampal brain slices were exposed varying concentrations at...

Examine the effect of preoperative dose IV ibuprofen on stress response and postoperative recovery in laparoscopic cholecystectomy patients.Prospective, randomized, controlled, double-blind, multicenter trial.Three university-based, tertiary care hospitals.Fifty-five adults, ASA 1, 2, or 3 scheduled for were given a single placebo 800 mg.Neurobehavioral assessments evaluated preoperatively, PACU, POD 3, using 40-item Quality Recovery questionnaire (QoR40), 9-item Modified Fatigue Severity...

To determine emergency medicine (EM) residents' perceptions and reported practices of obtaining informed consent for department procedures.The authors performed a cross-sectional observational study EM residents. A brief, short-answer survey was distributed that covered the following topics related to consent: training, confidence comfort levels, current practices. Data were analyzed using basic frequency displays, descriptive statistics are reported.Of 20 programs contacted, 16 responded...

Providing anesthesia to patients with myotonic dystrophy (DM) can be very challenging due the multisystemic effects of disease and extreme sensitivity these sedatives, opioids, anesthetic agents. Other factors such as hypothermia, shivering, or mechanical electric stimulation during surgery precipitate myotonia which is difficult abolish lead further complications. Generally, local regional preferred avoid complications associated general in this group. However there are several case reports...

Reflex sympathetic dystrophy (RSD) may co-occur with posttraumatic stress disorder (PTSD). a case study is reported of challenging adolescent patient who presented to chronic pain service RSD and PTSD. multidisciplinary approach utilizing nerve-block therapy adjunctive pharmacologic treatment, physical rehabilitation. behavioral/cognitive psychological was employed produce significant reduction in as well more physically psychologically functional adolescent. The diagnosis treatment each...

We report the use of celiac plexus block (CPB) as a diagnostic aid in evaluation median arcuate ligament syndrome (MALS) pediatric patient. MALS is rare cause severe, debilitating, abdominal postprandial pain associated with nausea, vomiting, occasional diarrhea, unintentional weight loss, and bruit. diagnosis exclusion, after multiple investigations. This first case where CPB was used to confirm candidacy for corrective surgery population has served cornerstone MALS.

The combination of 2-chloroprocaine and bupivacaine (C/B) for regional anesthesia has been described, but its use was largely abandoned due to equivocal results in efficacy. In this prospective, double-blind, randomized study, we compared the onset an interscalene block using C/B versus a lidocaine (L/B).Thirty patients scheduled shoulder arthroscopy under were divided into two groups 15 each. One group received 3% combined with bicarbonate epinephrine, immediately followed by 0.5% whereas...

There is an increasing use of electronic health records in hospitals across the United States. The speed and accuracy residents documenting has been insufficiently addressed.We studied resident typing skills at New York Methodist Hospital. Participating typed a standard 100-word alphanumerical paragraph patient's medical history. Typing were assessed by calculating net words per minute (WPM). categorized as follows: (1) fewer than 26 WPM very slow; (2) to 35 (3) 45 intermediate; (4) greater...

Anti-fibrotic and tissue regenerative mesenchymal stromal cell (MSC) properties are largely mediated by secreted cytokines growth factors. MSCs implanted to augment joint cartilage replacement treat diabetic ulcers burn injuries simultaneously with local anesthetics, which reduce pain. However, the effect of anesthetics on therapeutic human MSC secretory function has not been evaluated. In order assess secretome, a panel four different potencies - lidocaine, procaine, ropivacaine bupivacaine...

CATATONIA presents with stupor, excitement, or alternating stupor and excitement 1 covers a broad group of movement disorders sometimes seen in psychotic illness. It is most often associated schizophrenia but can also be found connection mania depression. 2 Other conditions which catatonia present include neurologic disorders, systemic metabolic as side effect certain medications. Any two the following signs manifests catatonia: motor immobility, excessive activity, negativism mutism,...

Acute postoperative pain remains inadequately managed. Although patient-controlled analgesia (PCA) represents a significant advance in management, drawbacks may include invasiveness and the potential for programming errors. The analysis presented here is based on pooled patient-level safety data from four multicenter, randomized, active-controlled trials that evaluated tolerability of needle-free, preprogrammed fentanyl HCl iontophoretic transdermal system (ITS) versus morphine intravenous...

The laryngeal mask airway (LMA) provides a safe and effective method of securing an in anesthesia critical care settings. Although the classic LMA airtight seal over glottic opening to provide gas exchange, several modifications, additions, variations have been developed are currently use. In particular, Fastrach LMA, through which endotracheal tube can be introduced, alternative for intubation difficult patient. LMAs also life-saving facilitating temporary field or interhospital transport....

This study explored the effects of propofol on c-Fos and Egr-1 in neuroblastoma (N2A) cells. We demonstrate that induced expression within 30 60 min exposure time. At 16.8 microM concentration, a 6 2.5-fold Egr-1, respectively. However, at concentrations above 100 microM, failed to induce or Egr-1. Propofol-induced transcription was unaffected by bicuculline, gamma-aminobutyric acid-A receptor antagonist, but abolished PD98059, mitogen-activated protein kinase/extracellular signal-regulated...