A5059343866- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Chronic Lymphocytic Leukemia Research
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Cell death mechanisms and regulation
- Lymphoma Diagnosis and Treatment
- Extracellular vesicles in disease
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- Glycosylation and Glycoproteins Research
- CAR-T cell therapy research
- Neonatal Respiratory Health Research
- Glioma Diagnosis and Treatment
- Toxin Mechanisms and Immunotoxins
- Phagocytosis and Immune Regulation
- Blood groups and transfusion
- Cancer Immunotherapy and Biomarkers
- Peptidase Inhibition and Analysis
- Pneumonia and Respiratory Infections
- Retinoids in leukemia and cellular processes
- Acute Lymphoblastic Leukemia research
University of Kentucky
2015-2024
Markey Cancer Center
2012-2024
Sri Krishnadevaraya University
2024
Laboratory of Molecular Genetics
2007-2022
Cancer Research Center
2008-2014
University of Louisville
2010
Sapienza University of Rome
2010
Bipar
2001-2008
University of Pennsylvania
1982-2008
The Graduate Center, CUNY
2006-2008
A subset of B lymphocytes present primarily in the peritoneal and pleural cavities is defined by expression CD5 elevated autoimmune diseases. Upon signaling through membrane immunoglobulin M (mIgM), splenic (B-2) proliferate, whereas cells (B-1) undergo apoptosis. However, CD5-deficient mice, B-1 responded to mIgM crosslinking developing a resistance apoptosis entering cell cycle. In wild-type cells, prevention association between rescued their growth response crosslinking. Thus...
Lung cancer is the leading cause of cancer-related deaths worldwide. Non-small-cell lung (NSCLC) most common type accounting for 84% all cancers. Paclitaxel (PAC) a widely used drug in treatment broad spectrum human cancers, including lung. While efficacious, PAC generally not well tolerated and its limitations include low aqueous solubility, significant toxicity. To overcome dose-related toxicity solvent-based PAC, we utilized bovine colostrum-derived exosomes as delivery vehicle cancer....
Rapamycin, a potent immunosuppressive drug that prevents rejection of organ transplants in many animals, caused profound growth inhibition an immature B cell lymphoma, BKS-2, at very low concentrations (2 ng/ml). Similar was also observed series lymphomas (i.e., L1.2, NFS.1.1, and WEHI-279) as well thymoma cells. The death induced by rapamycin BKS-2 lymphoma found to be via programmed death, or apoptosis. In contrast rapamycin, neither FK506 nor CsA affected the normal these FK506, but not...
Aged humans and rodents are susceptible to infection with Streptococcus pneumoniae bacteria as a result of an inability make antibodies capsular polysaccharides. This is partly decreased production proinflammatory cytokines increased interleukin (IL)-10 by macrophages (Mphi) from aged mice. To understand the molecular basis cytokine dysregulation in mouse Mphi, microarray analysis was performed on RNA resting lipopolysaccharide (LPS)-stimulated Mphi control mice using Affymetrix Mouse Genome...
Abstract A reduction in macrophage (MΦ) function with aging makes mice less responsive to bacterial capsular polysaccharides, such as those present the pneumococcal polysaccharide vaccine, a model of thymus independent (TI) antigen (Ag). Using trinitrophenol (TNP)-lipopolysaccharide (LPS) and TNP-Ficoll, two other well-studied TI Ag, we studied mechanistic basis reduced MΦ aged. We show that aged are profoundly hyporesponsive these Ag. As result requirement for MΦ, highly purified B cells...
Abstract Neonates do not respond to thymus-independent (TI) antigens (Ag), making them vulnerable infection with encapsulated bacteria. The antibody (Ab) response of adult and neonatal B cells TI Ag requires certain cytokines, which are provided by T or macrophages (MΦ). Lipopolysaccharide (LPS) failed induce MΦ produce interleukin (IL)-1β tumor necrosis factor α (TNF-α) mRNA secrete IL-1β, IL-12, TNF-α. However, LPS induced neonates some IL-6 three- fivefold more IL-10 than adults....
Abstract B lymphomas account for the majority of lymphoma cases. BCR expression appears to be important because most oncogenes are translocated nonrearranged Ig loci and all variants that arise in anti-idiotypic Ab-treated patients remain positive. Based on this fact is required mature cell survival, we tested requirement continued growth survival cells. Using Igα or Igβ-specific small interfering RNA (siRNA) inhibit expression, demonstrate first time constitutive signaling by critical...
Withaferin A (WA), a withanolide from the plant, Ashwagandha (Withania somnifera) used in Ayurvedic medicine, has been found to be valuable treatment of several medical ailments. WA have anticancer activity against various solid tumors, but its effects on hematological malignancies not studied detail. strongly inhibited survival human and murine B cell lymphoma lines. Additionally, vivo studies with syngeneic-graft cells suggest that inhibits growth tumor does affect other proliferative...
The demise of B cell progenitors expressing functional IgM receptors for self appears to be the main mechanism by which tolerance is accomplished. genetic mechanisms that regulate death process during this critical step development are still poorly understood. We have studied regulation apoptosis in WEHI-231 lymphoma cells after treatment with a panel anti-IgM mAbs as an vitro model clonal deletion. showed product bcl-x, Bcl-xL, can inhibit anti-IgM-induced but not cycle arrest...
The Legionnaire's disease bacterium, Legionella pneumophila, is a facultative intracellular pathogen which invades and replicates within two evolutionarily distant hosts, free-living protozoa mammalian cells. Invasion replication are thought to be major factors in the transmission of disease. Although attachment invasion human macrophages by L. pneumophila mediated part complement receptors CR1 CR3, protozoan receptor involved bacterial has not been identified. To define molecular events we...
Abstract Curcumin (diferuloylmethane), a component of dietary spice turmeric (Curcuma longa), has been shown in recent studies to have therapeutic potential the treatment cancer, diabetes, arthritis, and osteoporosis. We investigated ability curcumin modulate growth B lymphomas. inhibited both murine human lymphoma vitro vivo. also demonstrate that curcumin-mediated inhibition is through survival kinase Akt its key target Bad. However, assays show not direct curcumin. identified novel for...
Monoclonal antibody to Lyb 2, a differentiation antigen present on all B cells, has been used study the role of 2 molecules in cell activation. anti-Lyb (m-anti-Lyb 2) transforms resting cells into blast and induces proliferation these activated cells. The proliferative response is property 5+ subset since fails stimulate from mice expressing CBA/N immune defect. by mature antibody-secreting added presence T cell-replacing factors contained supernatants concanavalin A-activated Thus, may...
Abstract Chronic lymphocytic leukemia (CLL) patients progressively develop an immunosuppressive state. CLL have more plasma IL-10, anti-inflammatory cytokine, than healthy controls. In vitro human cells produce IL-10 in response to BCR cross-linking. We used the transgenic Eμ–T cell oncogene-1 (TCL1) mouse model study role of associated immunosuppression. Eμ-TCL mice spontaneously because a B cell–specific expression oncogene, TCL1. Eμ-TCL1 constitutively which is further enhanced by...
Abstract Prostate apoptosis response-4 (Par-4) is a tumor-suppressor protein that induces in cancer cells, but not normal/immortalized cells. The cancer-specific proapoptotic action of Par-4 encoded its centrally located SAC domain. We report here the characterization novel mouse model with ubiquitous expression Although transgenic mice displayed normal development and life span, they were resistant to growth spontaneous, as well oncogene-induced, autochthonous tumors. Resistance...
Abstract Egr-1 (early growth response gene-1) is an immediate early gene encoding a zinc finger motif-containing transcription factor. Upon cross-linking of BCR, mature B cells undergo proliferation with increase in message. Immature lymphoma that express message and protein constitutively are inhibited when down-regulated by negative signals from BCR or antisense oligonucleotides. To test the hypothesis important for cell development, we examined primary secondary lymphoid organs Egr-1−/−...
Anti-Lyb-5.1 serum contains antibodies against two different B cell surface antigenic determinants, Lyb-5.1 and Lyb-7.1, which are defined in cytotoxicity functional tests, respectively. The antibody Lyb-7.1 is identified by its ability to specifically inhibit vitro primary responses TNP-Ficoll. can be made monospecific for anti-Lyb-7.1 activity absorption with spleen cells from AL/N mice have been typed as Lyb-5.1+, Lyb-7.1-. each under control of one or set closely linked genes. loci...
Lyb 5 is a B-cell alloantigen which expressed on 50-60% of B cells. It was defined originally the basis cytotoxicity. We have described new reactivity within anti-Lyb serum selective inhibition antibody responses in vitro by this antiserum absence complement. This inhibitory activity 5.1 appears to be due recognition antigenic determinants different from prototype antigens detected cytotoxicity assay. Anti-Lyb incorporated into spleen cell cultures selectively inhibits class...