A5012338889- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Nerve injury and regeneration
- Retinal Development and Disorders
- Immune Response and Inflammation
- Neurogenesis and neuroplasticity mechanisms
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Calpain Protease Function and Regulation
- NF-κB Signaling Pathways
- RNA regulation and disease
- Endoplasmic Reticulum Stress and Disease
- Adenosine and Purinergic Signaling
- Neuroscience and Neural Engineering
- Lipid Membrane Structure and Behavior
- Ubiquitin and proteasome pathways
- Photodynamic Therapy Research Studies
- GABA and Rice Research
- RNA Research and Splicing
- S100 Proteins and Annexins
University of Coimbra
2016-2025
University of Edinburgh
2024
University of Miami
2024
Centro de Neurociências e Biologia Celular
2003-2015
Center for Neurosciences
1996-2011
Biocant
2010
Academy of Athens
2009
University of Michigan
2007
Lundbeck (Denmark)
2006
Praxis
1998
Brain-derived neurotrophic factor (BDNF) plays an important role in synaptic plasticity the hippocampus, but mechanisms involved are not fully understood. The neurotrophin couples activation to changes gene expression underlying long term potentiation and short plasticity. Here we show that BDNF acutely up-regulates GluR1, GluR2, GluR3 α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor subunits 7-day tropomyosin-related kinase vitro cultured hippocampal neurons. increase...
GABAA receptors (GABAAR) are the major players in fast inhibitory neurotransmission central nervous system (CNS). Regulation of GABAAR trafficking and control their surface expression play important roles modulation strength synaptic inhibition. Different pieces evidence show that alterations distribution dysregulation turnover impair activity synapses. A diminished efficacy affects excitatory/inhibitory balance is a common feature various disorders CNS characterized by an increased...
Machado–Joseph disease is the most frequently found dominantly-inherited cerebellar ataxia. Over-repetition of a CAG trinucleotide in MJD1 gene translates into polyglutamine tract within ataxin 3 protein, which upon proteolysis may trigger disease. We investigated role calpains generation toxic fragments and pathogenesis For this purpose, we inhibited calpain activity mouse models by overexpressing endogenous calpain-inhibitor calpastatin. Calpain blockage reduced size number mutant...
Brain-derived neurotrophic factor (BDNF) plays an important role in neuronal survival through activation of TrkB receptors. The trkB gene encodes a full-length receptor tyrosine kinase (TrkB.FL) and its truncated (T1/T2) isoforms. We investigated the changes protein levels signaling activity under excitotoxic conditions, which are characteristic brain ischemia, traumatic injury, neurodegenerative disorders. Excitotoxic stimulation cultured rat hippocampal or striatal neurons downregulated...
BDNF is a pro-survival protein involved in neuronal development and synaptic plasticity. strengthens excitatory synapses contributes to LTP, presynaptically, through enhancement of glutamate release, postsynaptically, via phosphorylation neurotransmitter receptors, modulation receptor traffic activation the translation machinery. We examined whether upregulated vesicular (VGLUT) 1 2 expression, which would partly account for increased release LTP. Cultured rat hippocampal neurons were...
In Alzheimer's disease (AD), amyloid β (Aβ)-triggered cleavage of TrkB-FL impairs brain-derived neurotrophic factor (BDNF) signaling, thereby compromising neuronal survival, differentiation, and synaptic transmission plasticity. Using cerebrospinal fluid postmortem human brain samples, we show that occurs from the early stages increases as a function pathology severity. To explore therapeutic potential this mechanism, designed small TAT-fused peptides screened their ability to prevent...
Abstract The stability of expression an internal control is required for accurate and reliable normalization in quantitative real‐time reverse transcription–polymerase chain reaction (qRT‐PCR) experiments. However, commonly used reference genes can be regulated under specific experimental conditions, particularly response to stimuli that exert multiple effects on gene expression. neurotrophin brain‐derived neurotrophic factor (BDNF) regulates through activation signaling cascades, we have...
Machado–Joseph Disease (MJD) is the most prevalent autosomal dominantly inherited cerebellar ataxia. It caused by an expanded CAG repeat in ATXN3 gene, which translates into a polyglutamine tract within ataxin-3 protein. Present treatments are symptomatic and do not prevent disease progression. As calpain overactivation has been shown to contribute mutant proteolysis, translocation nucleus, inclusions formation neurodegeneration, we investigated potential role of inhibition as therapeutic...