A5069503065- Lymphatic System and Diseases
- Hippo pathway signaling and YAP/TAZ
- Planarian Biology and Electrostimulation
- Angiogenesis and VEGF in Cancer
- Sphingolipid Metabolism and Signaling
- Congenital heart defects research
- Tissue Engineering and Regenerative Medicine
- Cancer-related molecular mechanisms research
- Spaceflight effects on biology
- Electrospun Nanofibers in Biomedical Applications
Stanford University
2015-2023
Cardiovascular Institute of the South
2015
Sufficient blood flow to tissues relies on arterial vessels, but the mechanisms regulating their development are poorly understood. Many arteries, including coronary arteries of heart, form through remodeling an immature vascular plexus in a process triggered and shaped by flow. However, little is known about how cues from fluid shear stress translated into responses that pattern artery development. Here, we show mice lacking endothelial Dach1 had small decreased cell polarization, reduced...
The role of nanotopographical extracellular matrix (ECM) cues in vascular endothelial cell (EC) organization and function is not well-understood, despite the composition nano- to microscale fibrillar ECMs within blood vessels. Instead, predominant modulator EC traditionally thought be hemodynamic shear stress, which uniform stress induces parallel-alignment ECs with anti-inflammatory function, whereas disturbed flow a disorganized configuration pro-inflammatory function. Since acts on by...
Abstract One-way valves in the lymphatic system form from endothelial cells (LECs) during embryonic development and are required for efficient tissue drainage. Although fluid flow is thought to guide both valve formation maintenance, how this occurs at a mechanistic level remains incompletely understood. We built microfluidic devices that reproduce critical aspects of patterns found sites valvulogenesis. Using these devices, we observed LECs replicated early steps valvulogenesis: oriented...
The endothelial cells that line blood and lymphatic vessels undergo complex, collective migration rearrangement processes during embryonic development, are known to be exquisitely responsive fluid flow. At present, the molecular mechanisms by which sense flow remain incompletely understood. Here, we report both G-protein-coupled receptor sphingosine 1-phosphate 1 (S1PR1) its ligand (S1P) required for upstream of human microvascular in an vitro setting. These findings consistent with a model...
Cytosolic calcium (Ca2+) is a ubiquitous second messenger that influences numerous aspects of cellular function. In many cell types, cytosolic Ca2+ concentrations are characterized by periodic pulses, whose dynamics can influence downstream signal transduction. Here, we examine the general question how cells use pulses to encode input stimuli in context response lymphatic endothelial (LECs) fluid flow. Previous work shows flow regulates LECs and Ca2+-dependent signaling plays key role...
One-way valves within lymphatic vessels are required for the efficient drainage of fluids. Fluid flow is proposed to be a key cue in regulating both formation and maintenance valves. However, our knowledge, no previous study has systematically examined response LECs complex combination spatially temporally varying fluid flows that occur at vivo. We built an vitro microfluidic device reproduces aspects environment found Using this device, we wall shear stresses (WSSs) led upregulated...
Introduction: Spatial variations in fluid flow are thought to help trigger the formation of valves within blood and lymphatic circulatory systems during embryonic development. However, how physical stimulus drives collective cell movements changes gene expression that underlie valve is not known. This knowledge gap considerable importance since failure venous vessels significant a wide variety cardiovascular disease states. Methods: In this study, we developed vitro devices allow us...