A5030028139- Microbial infections and disease research
- Bacterial Infections and Vaccines
- Rabies epidemiology and control
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Bacillus and Francisella bacterial research
- Vector-Borne Animal Diseases
- Mycotoxins in Agriculture and Food
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Aquaculture disease management and microbiota
- Yersinia bacterium, plague, ectoparasites research
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Poxvirus research and outbreaks
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- Brucella: diagnosis, epidemiology, treatment
- Streptococcal Infections and Treatments
- Coccidia and coccidiosis research
- Virology and Viral Diseases
- Influenza Virus Research Studies
- Escherichia coli research studies
Institute for Veterinary Medical Research
2016-2025
University of Veterinary Medicine
2025
Hungarian Research Network
2021
Centre for Agricultural Research
2012-2020
Hungarian Academy of Sciences
2011-2020
National Food Chain Safety Office
2013
Magyar Agrár- és Élettudományi Egyetem
2013
University of Debrecen
2010
Colorado State University
1997
Biotechnology and Biological Sciences Research Council
1988
Q fever is an important zoonotic disease caused by Coxiella burnetii. There are few reliable data about C. burnetii infection available. The aim of this study was to assess the importance and potential infectious sources in Hungary. A total 215 milk samples (10 individual from each herd 1 bulk tank sample cattle herd), 400 serum (20 herd) were tested 15 dairy herds 5 sheep flocks located different parts found 19.3% (58/300) 38.0% (57/150) seropositivity cattle, 0% (0/100) 6.0% (3/50) sheep,...
ABSTRACT Bordetella bronchiseptica is one of the etiologic agents causing atrophic rhinitis and pneumonia in swine. It produces several purported virulence factors, including dermonecrotic toxin (DNT), which has been implicated turbinate atrophy seen cases rhinitis. The purpose these experiments was to clarify role this respiratory disease by comparing pathogenicity swine two isogenic dnt mutants their virulent DNT + parent strains. Two separate were performed, with each mutant-parent pairs....
Pasteurella multocida causes infectious diseases in a wide range of animal species. Antimicrobial therapy is still an effective tool for treatment. Generally, P. isolates are susceptible to most the widely used commercial antimicrobial agents but their excessive and unjustified use accelerates emergence resistant strains. We defined sensitivity pattern 56 strains isolated from poultry (20) swine [16 toxin (PMT) positive 20 PMT negative] 16 applied antibiotics (apramycin, cefquinome,...
Mycoplasma bovis is a worldwide pathogen, causative agent of pneumonia, mastitis, arthritis, and variety other symptoms in cattle. The economic losses due to mycoplasma pneumonia could be reduced by antibiotic treatment. aim the present study was determine vitro susceptibility M. strains isolated from cattle Hungary eleven antibiotics. Minimal inhibitory concentration (MIC) values 35 collected different parts between 2010 2013 were determined microbroth dilution method. Strains with high MIC...
In the recent years, African swine fever has become biggest animal health threat to industry. To facilitate quick genetic analysis of its causative agent, virus (ASFV), we developed a simple and efficient method for next generation sequencing viral DNA. Execution protocol does not demand complicated purification steps, enrichment by ultracentrifugation or DNA ASFV-specific PCRs, minimizes use Sanger sequencing. Efficient DNA-se treatment, monitoring sample preparation qPCR, whole genome...
African swine fever (ASF) is a highly lethal hemorrhagic viral disease that causes extensive economic and animal welfare losses in the Eurasian pig (Sus scrofa) population. To date, no effective safe vaccines have been marketed against ASF. A starting point for vaccine development using naturally occurring attenuated strains as base. Here, we aimed to remove multigene family (MGF) 110 gene of unknown function from Lv17/WB/Rie1 genome improve usability virus live-attenuated vaccine, reducing...
Background/Objectives: African swine fever virus (ASFV) is a devastating disease affecting domestic and wild suids causing significant economic losses in the global pig industry. Attenuated modified live (MLV) vaccines are most promising approaches for vaccine development. This study aimed to evaluate safety efficacy of four recombinant ASFV genotype II strains, derived from non-hemadsorbing (non-HAD) attenuated isolate Lv17/WB/Rie1, through single or simultaneous deletion...
A sudden mass mortality involving 44 fallow deer (Dama dama) occurred in September 2020 at a game management area near Budapest, Hungary. The die-off affected both sexes and all age classes equally. On postmortem examination of 2 subadults 1 fawn, we found average body condition, diffuse subcutaneous edema, swelling peripheral lymph nodes, marked hemorrhages nodes along the esophagus trachea, left ventricular dilation. We isolated Pasteurella-like colonies from spleen, liver, small...
Determining the in vitro susceptibility to 11 antibiotics of Francisella tularensis subsp. holarctica strains belonging phylogenetic group B.13, from different areas Hungary. Twenty-nine F. isolated between 2003 and 2010 free-ranging European brown hares (Lepus europaeus) a captive patas monkey (Erythrocebus patas) were collected parts Hungary examined for antibiotic with commercially available MIC test strips on modified Francis agar plates; values interpreted according CLSI breakpoints....
Several new taxa belonging to the genus Francisella have been described recently. The present study describes prevalence of tularensis and Francisella-like endosymbionts (FLE) in ticks collected from Hungary 2007 2009 characterizes genetic variability FLEs. A total 5402 Ixodid (Ixodes ricinus, I. acuminatus, Dermacentor marginatus, D. reticulatus, Haemaphysalis inermis, H. concinna, punctata) were vegetation animal hosts tested with conventional PCR, detecting 16S rRNA tul4 genes. F. ssp....
Mycoplasma hyopneumoniae has a primary role in the porcine respiratory disease complex (PRDC). The objective of this study was to determine whether fumonisin mycotoxins influence character and/or severity pathological processes induced lungs pigs by hyopneumoniae. Four groups ( n = 7/group) were used, one fed 20 ppm B 1 (FB ) from 16 days age (group F), only infected with M. on day 30 M), and group FB MF), along an untreated control C). Computed tomography (CT) scans (M MF) 44 demonstrated...
Riemerella anatipestifer causes disease in many avian species. A total of 185 R. strains isolated Hungary between 2000 and 2014 from geese ducks were tested against 13 antibiotics (ampicillin, doxycycline, enrofloxacin, erythromycin, florfenicol, flumequine, gentamicin, penicillin, spectinomycin, streptomycin, sulphamethoxazole—trimethoprim, sulphonamide compounds, tetracycline) by the Kirby-Bauer disk diffusion method. The majority susceptible to florfenicol (97.9%), ampicillin (95.1%),...
DATA REPORT article Front. Genet., 28 July 2020Sec. Genomic Assay Technology Volume 11 - 2020 | https://doi.org/10.3389/fgene.2020.00758
Lv17/WB/Rie1-Δ24 was produced via illegitimate recombination mediated by low-dilution serial passage in the Cos7 cell line and isolated on PAM culture. The virus contains a huge ~26.4 Kb deletion left end of its genome. Lv17/WB/Rie1-ΔCD-ΔGL generated homologous recombination, crossing two ASFV strains (Lv17/WB/Rie1-ΔCD Lv17/WB/Rie1-ΔGL containing eGFP mCherry markers) during co-infection. presence unique parental markers genome indicates at least events crossing, suggesting that is...
Ornithobacterium rhinotracheale is a widely distributed rod-shaped Gram-negative bacterium that infects several avian species including chickens and turkeys. It associated with respiratory signs, growth retardation, mortality, reduced egg production, thus causing severe economic losses to the poultry industries. In this study, 37 field isolates of O. rhinotracheale, collected from various locations in Hungary between 1997 2015, were identified characterized by analysis partial 16S rRNA gene...
The antimicrobial susceptibility of 19 Bordetella avium and 36 Ornithobacterium rhinotracheale strains was tested by the Kirby-Bauer disk diffusion method, minimal inhibitory concentrations (MIC) amoxicillin, doxycycline erythromycin were also determined. Most O. resistant to nalidixic acid, sulphamethoxazole-trimethoprim gentamicin, susceptible ampicillin, chloramphenicol, spectinomycin tilmicosin. All B. ceftiofur lincomycin doxycycline, polymyxin B, sulphonamides. MICs ranged widely for...