A5027983782- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Cellular Mechanics and Interactions
- Advanced Biosensing Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- biodegradable polymer synthesis and properties
- Hydrogels: synthesis, properties, applications
- Advanced biosensing and bioanalysis techniques
- Modular Robots and Swarm Intelligence
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Electrospun Nanofibers in Biomedical Applications
- Sirtuins and Resveratrol in Medicine
- Transgenic Plants and Applications
- Plant biochemistry and biosynthesis
- Enzyme Catalysis and Immobilization
- Neuroscience and Neural Engineering
- Algal biology and biofuel production
University of Washington
2018-2021
Seattle University
2019-2020
Most mono- and co-culture bioprocess applications rely on large-scale suspension fermentation technologies that are not easily portable, reusable, or suitable for on-demand production. Here, we describe a hydrogel system harnessing the bioactivity of embedded microbes small molecule peptide production in microbial mono-culture consortia. This platform bypasses challenges engineering multi-organism consortia by utilizing temperature-responsive, shear-thinning to compartmentalize organisms...
Additive manufacturing (AM) is energizing the fields of chemistry and materials science to develop new inks for applications within such as aerospace, robotics, healthcare. AM enables fabrication innumerable 3D geometries that cannot be easily produced by other means. In spite great promise an advanced form future manufacturing, there are still fundamental challenges with respect sustainability need addressed. Some material needs include sustainable sources printing inks, resins, filaments,...
Living materials, which are composites of living cells residing in a polymeric matrix, designed to utilize the innate functionalities address broad range applications such as fermentation and biosensing. Herein, we demonstrate additive manufacturing catalytically active materials (AMCALM) for continuous fermentation. A multi-stimuli-responsive yeast-laden hydrogel ink, based on F127-dimethacrylate, was developed printed using direct-write 3D printer. The reversible stimuli-responsive...
Resveratrol is a plant secondary metabolite with diverse, potential health-promoting benefits. Due to its nutraceutical merit, bioproduction of resveratrol via microbial engineering has gained increasing attention and provides an alternative unsustainable chemical synthesis straight extraction from plants. However, many studies on production were implemented the addition water-insoluble phenylalanine or tyrosine-based precursors medium, limiting in sustainable development bioproduction.Here...
Abstract Living materials are created through the embedding of live, whole cells into a matrix that can house and sustain viability encapsulated cells. Through immobilization these cells, their bioactivity be harnessed for applications such as bioreactors production high‐value chemicals. While interest in living is growing, many existing lack robust structure difficult to pattern. Furthermore, employ only one type microorganism, or microbial consortia with little control over arrangement...
Additive manufacturing allows three-dimensional printing of polymeric materials together with cells, creating living for applications in biomedical research and biotechnology. However, an understanding the cellular phenotype within is lacking, which a key limitation their wider application. Herein, we present approach to characterize materials. We immobilized budding yeast Saccharomyces cerevisiae three different photo-cross-linkable triblock hydrogels containing F127-bis-urethane...
The three-dimensional (3D) printing of cell-containing polymeric hydrogels creates living materials (LMs), offering a platform for developing innovative technologies in areas like biosensors and biomanufacturing. polymer material properties cross-linkable F127-bis-urethane methacrylate (F127-BUM) allow reproducible 3D stability physiological conditions, making it suitable fabricating LMs. Though F127-BUM-based LMs permit diffusion solute molecules glucose ethanol, remains unknown whether...
Traditional production of industrial and therapeutic proteins by eukaryotic cells typically requires large-scale fermentation capacity. As a result, these systems are not easily portable or reusable for on-demand protein applications. In this study, we employ Bioproduced Proteins On Demand (Bio-POD), F127-bisurethane methacrylate hydrogel-based technique that immobilizes engineered
Abstract We describe the synthesis, characterization and direct‐write 3D printing of triblock copolymer hydrogels that have a tunable response to temperature shear stress. In aqueous solutions, these polymers utilize temperature‐dependent self‐association poly(alkyl glycidyl ether) ‘A’ blocks central poly(ethylene oxide) segment create physically crosslinked three‐dimensional network. The was dependent upon composition, chain length concentration block in copolymer. Rheological experiments...
Herein, we describe a method to produce yeast-laden hydrogel inks for the direct-write 3D printing of cuboidal lattices immobilized whole-cell catalysis.
Herein, we describe a yeast-laden hydrogel ink that can be printed using direct-write 3D printer and used for the production of peptide. A poly(alkyl glycidyl ether)-based triblock copolymer was synthesized formulated as characterized via rheometry to evaluate printability ink. An engineered yeast strain with an upregulated α-factor pathway incorporated into printed. The immobilized cells exhibited adequate viability 87.5% within hydrogel. up-regulated α- factor detected...
Abstract Additive manufacturing allows three-dimensional printing of polymeric materials together with cells, creating living for applications in biomedical research and biotechnology. However, understanding the cellular phenotype within is lacking a key limitation their wider application. Herein, we present an approach to characterize materials. We immobilized budding yeast Saccharomyces cerevisiae three different photocross-linkable triblock hydrogels containing F127-bis-urethane...
Abstract The three-dimensional printing of cells offers an attractive opportunity to design and develop innovative biotechnological applications, such as the fabrication biosensors or modular bioreactors. Living materials (LMs) are cross-linked polymeric hydrogel matrices containing cells, recently, one most deployed LMs consists F127-bis-urethane methacrylate (F127-BUM). material properties F127-BUM allow reproducible 3D stability in physiological environments. These permissible for small...