A5048399550- Celiac Disease Research and Management
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Gout, Hyperuricemia, Uric Acid
- Microscopic Colitis
- Liver Disease Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Folate and B Vitamins Research
- Cerebrovascular and Carotid Artery Diseases
- Venous Thromboembolism Diagnosis and Management
- Adenosine and Purinergic Signaling
- Eosinophilic Esophagitis
- Diabetes and associated disorders
- Liver Diseases and Immunity
- Antioxidant Activity and Oxidative Stress
- Amino Acid Enzymes and Metabolism
- Helicobacter pylori-related gastroenterology studies
- Cancer, Lipids, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Metabolomics and Mass Spectrometry Studies
- Microbial Metabolites in Food Biotechnology
- Hormonal and reproductive studies
- Enzyme Structure and Function
- Advanced Proteomics Techniques and Applications
University of Siena
2011-2024
Azienda Ospedaliera Universitaria Senese
2013-2024
Ospedale Santa Maria alle Scotte
2020-2024
Università Cattolica del Sacro Cuore
1999
Karolinska Institutet
1993
Celiac disease (CD) is an immune-mediated intolerance to dietary gluten, affecting genetically predisposed individuals. ELISA-based serological tests help decide if further duodenal biopsy necessary, for this the diagnostic kits have be accurate, specific, and sensible. In study, we investigate performance of ELISA that uses purified cross-linked complex tissue transglutaminase gliadin, referred as "neoepitope" (AESKULISA® tTG New Generation), antigen.We evaluated 41 newly diagnosed celiac...
Autoantibodies against-phospholipase A2 receptor (PLA2R) are specific markers of idiopathic membranous nephropathy (iMN). Enzyme-linked immunosorbent assay (ELISA) is becoming the preferred method in many laboratories for determination anti-PLA2R antibodies, because it provides quantitative results, and not prone to subjective interpretation, as case with indirect immunofluorescence assay.The purpose our study was determine diagnostic performance serum PLA2R antibodies detected by...
Background It is important to have methods for evaluating dietary compliance in patients with celiac disease (CD). Determination of fecal gluten immunogenic peptides (GIPs) was recently proposed as a method detecting intake. The aim this study evaluate whether determination GIPs can be used an indicator gluten-free diet (GFD). Methods Twenty-five persons CD on at least one year were enrolled the study. Compliance assessed by Biagi questionnaire, evaluation symptoms and assay IgA anti-tissue...
Anti-double-stranded DNA antibodies (anti-dsDNA) are considered a specific marker for systemic lupus erythematosus (SLE). Though the Farr technique was once reference method their detection, it has been almost entirely replaced by more recently developed assays. However, there is still no solid evidence of commutability these methods in terms diagnostic accuracy and correlation with Crithidia luciliae immunofluorescence test (CLIFT). Anti-dsDNA antibody levels were measured 80 subjects: 24...
In previous studies, we found that castration induced interesting morphological and biochemical changes in rat liver. For the present study, have examined effects of testosterone on kinetics purine nucleotide metabolism with aim determining steps affected by deficiency. A biomathematical model was used to analyze many reactions involved. The simplifies four main steps: 1) de novo synthesis from PRPP IMP; 2) inosinic branch point IMP GMP or AMP; 3) catabolism IMP, AMP uric acid; 4) RNA DNA...
Heparan sulfate proteoglycans, HSPGs, modulate major transformations of cancer cells, leading to tumor growth, invasion and metastasis. HSPGs also regulate neo-angiogenesis which prompts progression metastatic spread. A different aspect heparin analogs is their prominent role in the coagulation blood. The interplay between metastasis being actively studied: anticoagulants such as heparin-derivatives have anticancer activity procoagulants, thrombin, positively proliferation, migration...
Serum autoantibodies specifically directed toward intracellular cytoskeletal actin filaments (anti-actin antibodies, AAA) were found to be associated with intestinal villous atrophy (IVA) in celiac disease (CD). The aim of this study was assess IgA-AAA a commercial test that uses sections rat epithelial cells well-selected cohort patients and evaluate the relationship between presence serum severity mucosa damage.Serum samples from 70 CD 150 controls subjects analyzed retrospectively for...
This study was carried out on carotid artery plaque and plasma of 50 patients. We analyzed uric acid, hypoxanthine, xanthine, allantoin levels to verify if enzymatic purine degradation occurs in advanced plaque; we also determined free radicals sulphydryl groups check there is a correlation between oxidant status catabolism. Comparing found higher radicals, decrease some protectors, such as plaque. observed very important phenomenon plaque, the presence due chemical oxidation since humans do...
Free radical excess and oxidative stress are implicated in the formation progression of atherosclerotic plaque through actions on susceptible vascular cells, such as by activating xanthine oxidase. Purine bases other antioxidant compounds could play important protective roles atherogenesis, nonenzymatic low molecular weight thiol defenses, not previously evaluated carotid artery plaque. Therefore, we measured purine catabolites (hypoxanthine, xanthine, uric acid, allantoin) (total sulphydryl...