A5040874943- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Polymer Surface Interaction Studies
- Electrochemical Analysis and Applications
- Luminescence and Fluorescent Materials
- Soil Carbon and Nitrogen Dynamics
- Adhesion, Friction, and Surface Interactions
- Lipid Membrane Structure and Behavior
- Surface Modification and Superhydrophobicity
- Gold and Silver Nanoparticles Synthesis and Applications
- Image Processing Techniques and Applications
- Advanced Image Fusion Techniques
- Power System Optimization and Stability
- Nanopore and Nanochannel Transport Studies
- Smart Agriculture and AI
- Agricultural Science and Fertilization
- Microplastics and Plastic Pollution
- Advanced Chemical Sensor Technologies
- Marine Biology and Environmental Chemistry
- Clay minerals and soil interactions
- Bacteriophages and microbial interactions
- Solid State Laser Technologies
- Optical measurement and interference techniques
China University of Geosciences
2011-2025
Taiyuan University of Technology
2025
Guangxi Normal University
2024
Hebei Agricultural University
2024
Tianjin Research Institute of Water Transport Engineering
2023
Sichuan University
2016-2021
Chengdu University
2016
Chinese Academy of Sciences
2006-2015
Academia Sinica
2009
Shanghai Institute of Optics and Fine Mechanics
2006
An ortho-nitrobenzyl phosphate ester-caged nucleic acid hairpin structure coupled to the CRISPR-Cas12a complex is introduced as a functional reaction module for light-induced activation of (LAC12a) machinery toward amplified fluorescence detection microRNA-21 (miRNA-21). The LAC12a applied selective, in vitro sensing miRNA-21 and intracellular imaging different cell lines. system used image cycle phases MCF-7 cells. Moreover, integrated cells enables two-photon laser confocal...
A rapid signal amplification system based on the self-replicating catalyzed hairpin assembly is reported in which two hairpins, H1 and H2, were well-designed split target/trigger DNA G-quadruplex sequences respectively integrated into H2. Target/trigger can be cyclically used this to form duplex assemblies (H1–H2), will bring fragments close-enough proximity induce formation of intact as a colorimetric readout. Similarly, reunite sequence that identical DNA; then, obtained replica also new...
Specific and sensitive detection imaging of cancer-related miRNA in living cells are desirable for cancer diagnosis treatment. Because the spatiotemporal variability expression level during different cell cycles, signal amplification strategies that can be activated by external stimuli required to image miRNAs on demand at desired times selected locations. Herein, we develop a strategy termed as photoactivated DNA walker based nanoflares, which enables photocontrollable single cells. The...
Abstract Cytochrome c (Cyt. ) is a key initiator of the caspases that activate cell apoptosis. The spatiotemporal evaluation contents Cyt. in cellular compartments and detection delivery between upon apoptosis important for probing viabilities. We introduce an optical probe electrochemical quantitative assessment at single level. or probes are functionalized with photoresponsive o‐nitrobenzylphosphate ester‐caged aptamer constituents. These uncaged by light stimuli compartments, allowing...
The subcellular distribution of adenosine 5′-triphosphate (ATP) and the concentration ATP in living cells dynamically fluctuate with time during different cell cycles. dictated activation biosensing process enables spatiotemporal target detection single cells. Herein, a kind o-nitrobenzylphosphate ester hairpin nucleic acid was introduced as photoresponsive DNA probe for light-activated Two methods to spatiotemporally activate were discussed. One method usage micrometer-sized optical fiber...
It is known that the adsorption of short single-stranded DNA (ssDNA) on unmodified gold nanoparticles (AuNPs) much faster than for long ssDNA, and thus leads to length-dependent AuNPs aggregation after addition salt, color solutions sequentially changed from red blue in accordance with increase ssDNA length. However, we found herein sticky end hairpin exhibited a completely different behavior compared an inverse blue-to-red variation was observed colloid solution length when within certain...
The expression level and subcellular distribution of mRNA dynamically changed during the different cell circles. Spatiotemporally controllable signal amplification methods capable controlling when where process could allow sensitive imaging selected living cells at dictated time-intervals life-cycle. However, present for amplified are hard to control due lack an effective strategy precisely trigger process. Herein, we a conceptual study termed as photocontrollable nucleic acid cascade...
One-dimensional DNA polymer chains were obtained based on the catalyzed hairpin assembly and sticky end self-assembly, which led to a signal amplified colorimetric nucleic acid assay.
The natural clay resources that filled the impermeability requirement in Loess Plateau are scarce. Therefore, it is necessary to modify loess soil meet requirements of landfill barriers. In this study, an experimental study was performed determine effect different application rates (1%, 3%, and 7%) fly ash, nano silica sol, nanoclay amendments on hydraulic conductivity loess. Also, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM),...
A new detector, silvering detection window and in‐capillary optical fiber light‐emitting diode‐induced fluorescence detector (SDW‐ICOF‐LED‐IFD), is introduced for capillary electrophoresis (CE). The strategy of the work was that half surface coated with silver mirror, which could reflect undetected to photomultiplier tube be detected, consequently enhancing sensitivity. Sulfonamides (SAs) are important antibiotics achieved great applications in many fields. However, they pose a serious...
Abstract Background Pepper Phytophthora blight is a devastating disease during the growth process of peppers, significantly affecting their yield and quality. Accurate, rapid, non-destructive early detection pepper great importance for production management. This study investigated possibility using multispectral imaging combined with machine learning to detect in peppers. Peppers were divided into two groups: one group was inoculated blight, other left untreated as control. Multispectral...
Abstract Increasing use of ion-selective electrodes (ISEs) in the biological and environmental fields has generated demand for high-sensitivity ISEs. However, improving sensitivities ISEs remains a challenge because limit Nernstian slope (59.2/n mV). Here, we present universal ion detection method using an electronic integrated multi-electrode system (EIMES) that bypasses 59.2/n mV, thereby enabling substantial enhancement sensitivity The results reveal response is greatly increased from...