A5069986581- Fecal contamination and water quality
- Viral gastroenteritis research and epidemiology
- Environmental Justice and Health Disparities
- Urban Stormwater Management Solutions
- Child Nutrition and Water Access
- Groundwater flow and contamination studies
- Microbial Community Ecology and Physiology
- Pharmaceutical and Antibiotic Environmental Impacts
- Water Treatment and Disinfection
- Electrokinetic Soil Remediation Techniques
- Marine and coastal plant biology
- Marine Biology and Ecology Research
- Gut microbiota and health
- Molecular Biology Techniques and Applications
- Environmental Toxicology and Ecotoxicology
- Pesticide and Herbicide Environmental Studies
- Toxic Organic Pollutants Impact
- Plant and animal studies
- Ecology and Vegetation Dynamics Studies
- Freshwater macroinvertebrate diversity and ecology
- Diatoms and Algae Research
- Microplastics and Plastic Pollution
- Environmental DNA in Biodiversity Studies
- Insect-Plant Interactions and Control
- Wildlife-Road Interactions and Conservation
Sydney Water
2018-2024
Newcastle University
1978-1981
The study assessed the performance characteristics of host sensitivity, specificity and concentration for seven human wastewater- six animal scat-associated marker genes by analysing wastewater scat samples from urban catchments mega-coastal city Sydney, Australia. Absolute sensitivity was exhibited across three criteria used to assess wastewater-associated cross-assembly phage (CrAssphage), adenovirus (HAdV), Bacteroides HF183 (HF183), polyomavirus (HPyV), Lachnospiraceae (Lachno3),...
The HF183 marker gene, derived from the 16S rRNA gene of Bacteroides dorei, has been widely used to identify sewage pollution in environmental waters. CrAssphages are recently discovered DNA bacteriophages that highly abundant untreated and have shown promises for tracking contamination In this paper, we report development a duplex quantitative PCR (qPCR) assay simultaneous quantification crAssphage CPQ_056 genes impacted stormwater. Same primer probe sequences were qPCR as published simplex...
Quantitative microbial risk assessment (QMRA) of human health risks using fecal marker genes (HFMGs) is an useful water quality management tool. To inform accurate QMRA analysis, generation probability distribution functions for HFMGs, and reference pathogenic viruses can be improved by input correlation ratios based upon measurement HFMGs gene copies (GC) in untreated wastewater. The concentrations four (Bacteroides HF183, Lachnospiraceae Lachno3, CrAssphage pepper mild mottle virus...
This research investigated the in-situ decay rates of four human wastewater-associated markers (Bacteroides HF183 (HF183), Lechnospiraceae Lachno3 (Lachno3), cross-assembling phage (crAssphage), pepper mild mottle virus (PMMoV) and three enteric viruses (human adenovirus 40/41 (HAdV 40/41), enterovirus (EV) norovirus GII (HNoV GII) in estuarine water environments temperate Sydney, NSW, Australia, employing qPCR RT-qPCR assays. The study also aimed to compare observed mesocosms with...
This study establishes site-specific risk-based thresholds (RBTs) for sewage-associated markers, including Bacteroides HF183 (HF183), Lachnospiraceae Lachno3 (Lachno3), cross-assembly phage (CrAssphage), and pepper mild mottle virus (PMMoV), utilizing quantitative microbial risk assessment (QMRA) recreational estuarine waters (EW). The QMRA model simulates health risks from ingestion of EW contaminated with untreated sewage. RBTs were estimated considering decay rates concentrations markers...
Abstract This study investigated the magnitude of wet weather overflow (WWO)-driven sewage pollution in an urban lake (Lake Parramatta) located Sydney, New South Wales, Australia. Water samples were collected during a dry period and after two storm events, tested for range novel established sewage- [ Bacteroides HF183, crAssphage CPQ_056 pepper mild mottle virus (PMMoV)] animal feces-associated ( BacCan-UCD, cowM2 Helicobacter spp. associated GFD) microbial source tracking marker genes along...
Public complaints arise from visible gross pollutants deposited on receiving waterway margins and in associated riparian vegetation after sewer overflows. The current study was conducted to assess the capability of a modified commercial stormwater trash-net capture that spill overflows sanitary (separate stormwater) system. Trash nets were installed four with differing gauged frequencies volumes. Five categories (wet wipes paper products, items, condoms, miscellaneous identifiable matted...
This study investigated the decay rates of wastewater-associated markers and enteric viruses in laboratory microcosms mimicking estuarine water environments temperate Sydney, NSW, Australia using qPCR RT-qPCR assays. The results demonstrated reduction concentrations Bacteroides HF183, Lachnospiraceae Lachno3, cross-assembly phage (crAssphage), pepper mild mottle virus (PMMoV), human adenovirus (HAdV 40/41), enterovirus (EV) over a span 42 days under spring/summer temperatures,...
Feeding preference tests confirm the existence of an induced food plant in Carausius morosus (Br.) dual and multiple choice tests. The appears to be between ages 10 20 days remains at a very high level for remainder insect's life span. A statistical test concordance indicates that there is less flexibility older animals. Preferences can rapidly modified through feeding on second age differences are apparent throughout study. ZUSAMMENFASSUNG ENTWICKLUNG EINER INDUZIERTEN NAHRUNGSPRÄFERENZ BEI...
The current microbial source tracking (MST) study tracked the reduction of culturable fecal indicator bacteria enterococci, four human markers (Bacteroides HF183, Lachnospiraceae Lachno3, cross-assembly phage (CrAssphage) and pepper mild mottle virus (PMMoV)) along with enteric viruses - adenovirus 40/41 (HAdV 40/41), enterovirus (EV), norovirus GI (HNoV GI) GII GII) post wet weather overflows (WWOs) at two estuarine water sites from depths under separate six-day sampling campaigns over...
Four representative sites in the greater city of Sydney, Australia, were selected for a study wet-weather overflow sanitary (separate to stormwater) sewerage systems. Water samples collected by autosamplers from up eight wet weather events over 16 months and companion receiving water sites. The objective was identify risks posed sewage contaminants aquatic biota waters, aid prioritising management actions. Twelve organic identified influents across four under rainfall ingress diluted...
Macroinvertebrate surveys are commonly used for assessing the health of freshwater systems around world. Traditionally, surveying involves morphologically identifying families, and sometimes genera, present in samples. Biological indices, derived from taxonomic lists, provide convenient ways to summarise community data may be fairly insensitive species-level changes compositions. In recent years, molecular techniques taxa have become increasingly popular metabarcoding approaches that offer...
Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI
Sewer overflows are an environmental concern due to their potential introduce contaminants that can adversely affect downstream aquatic ecosystems. As these occur during rainfall events, the influence of rainwater ingress from inflow and infiltration on raw untreated wastewater (influent) within sewer is a critical factor influencing dilution toxicity contaminants. The Vineyard carrier in greater city Sydney, Australia, was selected for ecotoxicological investigation sanitary (separate...