A5007092930- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- DNA Repair Mechanisms
- Neurobiology and Insect Physiology Research
- Cancer Cells and Metastasis
- Invertebrate Immune Response Mechanisms
- Immune cells in cancer
- Silk-based biomaterials and applications
- Genomics and Phylogenetic Studies
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- COVID-19 and Mental Health
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- COVID-19 Pandemic Impacts
- Adipokines, Inflammation, and Metabolic Diseases
- Mycobacterium research and diagnosis
- Genomics, phytochemicals, and oxidative stress
- Protease and Inhibitor Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Adipose Tissue and Metabolism
- Aquaculture disease management and microbiota
- Fungal and yeast genetics research
- Microtubule and mitosis dynamics
- Extracellular vesicles in disease
Beijing Academy of Science and Technology
2024-2025
Institute of New Materials
2025
The University of Texas MD Anderson Cancer Center
2020-2024
Xijing Hospital
2019-2022
Tsinghua University
2015-2021
Air Force Medical University
2019
University of Florida
2010-2011
Peking University
2010
Kettering University
2004
Johns Hopkins University
2002-2004
Evaluation of potential immunity against the novel severe acute respiratory syndrome (SARS) coronavirus that emerged in 2019 (SARS-CoV-2) is essential for health, as well social and economic recovery. Generation antibody response to SARS-CoV-2 (seroconversion) may inform on acquired from prior exposure, antibodies spike protein receptor binding domain (S-RBD) are speculated neutralize virus infection. Some serology assays rely solely nucleocapsid (N-protein) detection antigen; however,...
The outbreak the SARS-CoV-2 (CoV-2) virus has resulted in over 6.5 million cases of COVID19, greatly stressing global healthcare infrastructure. Lacking medical prophylactic measures to combat disease spread, many nations have adopted social distancing policies order mitigate transmission CoV-2. While mathematical models suggested efficacy curb spread CoV-2, there is a lack systematic studies quantify real-world these approaches. Here, we first demonstrate that implementation US states...
The silent information regulator protein (Sir2) and its homologs are NAD+-dependent deacetylase enzymes that play important roles in a variety of physiological processes. However, the functions Sir2 family plants poorly understood. Here, we report Arabidopsis AtSRT2, homolog yeast Sir2, negatively regulates plant basal defense against pathogen Pseudomonas syringae pv. tomato DC3000 (PstDC3000). In response to PstDC3000 infection, expression AtSRT2 was down-regulated salicylic acid...
Exit of secretory cargo from the endoplasmic reticulum (ER) takes place at specialized domains called ER exit sites (ERESs). In mammals, loss TANGO1 and other MIA/cTAGE (melanoma inhibitory activity/cutaneous T cell lymphoma–associated antigen) family proteins prevents large cargoes such as collagen. Here, we show that Drosophila melanogaster Tango1, only member in fruit flies, is a critical organizer ERES–Golgi interface. Tango1 rings hold COPII (coat protein II) carriers Golgi close...
Abstract Cancer models play critical roles in basic cancer research and precision medicine. However, current vitro are limited by their inability to mimic the three-dimensional architecture heterogeneous tumor microenvironments (TME) of vivo tumors. Here, we develop an innovative patient-specific lung assembloid (LCA) model using droplet microfluidic technology based on a microinjection strategy. This method enables precise manipulation clinical microsamples rapid generation LCAs with good...
Origins of DNA replication in Schizosaccharomyces pombe lack a specific consensus sequence analogous to the Saccharomyces cerevisiae autonomously replicating ( ARS ) consensus, raising question how they are recognized by machinery. Because all well characterized S. origins located intergenic regions, we analyzed properties and biological activity such regions. The AT content intergenes is very high (≈70%), runs A's or T's occur with significantly greater frequency than expected....
Signalling networks that control the life or death of a cell are central interest in modern biology. While defined roles c-Jun N-terminal kinase (JNK) pathway regulating have been well-established, additional factors modulate JNK-mediated yet to be fully elucidated. To identify novel regulators JNK-dependent death, we performed dominant-modifier screen Drosophila and found Toll participates death. Loss signalling suppresses ectopically physiologically activated JNK signalling-induced Our...
Basement membranes (BMs) are thin sheet-like specialized extracellular matrices found at the basal surface of epithelia and endothelial tissues. They have been conserved across evolution required for proper tissue growth, organization, differentiation maintenance. The major constituents BMs two independent networks Laminin Type IV Collagen in addition to proteoglycan Perlecan glycoprotein Nidogen/entactin (Ndg). ability Ndg bind vitro Laminin, both with key functions during embryogenesis,...
ABSTRACT The outbreak the SARS-CoV-2 (CoV-2) virus has resulted in over 2.5 million cases of COVID19, greatly stressing global healthcare infrastructure. Lacking medical prophylactic measures to combat disease spread, many nations have adopted social distancing policies order mitigate transmission CoV-2. While mathematical models suggested efficacy curb spread CoV-2, there is a lack systematic studies quantify real-world these approaches. Here, we rate COVID19 before and after national were...
To investigate potential structures of d(CGG/CCG)n that might relate to their biological function and association with triplet repeat expansion diseases (TREDs), the structure a single-stranded (ss) oligonucleotide containing d(CCG)15 [ss(CCG)15] was examined by studies pH temperature dependence electrophoretic mobility, UV absorbance, circular dichroism, chemical modification, P1 nuclease digestion. ss(CCG)15 had an unusually high pKa (7.7 ± 0.2). At 8.5, formed relatively unstable (Tm = 30...
ABSTRACT Mycobacteria include a large number of pathogens. Identification to species level is important for diagnoses and treatments. Here, we report the development Web-accessible database hsp65 locus sequences ( http://msis.mycobacteria.info ) from 149 out 150 Mycobacterium species/subspecies. This can serve as reference identifying species.
Objective and aims: Osteopontin (OPN), an oxidant stress sensitive cytokine, plays a central role in liver fibrosis. While OPN expression can be reduced by small interfering RNA (siRNA), the challenge to deliver siRNA safely effectively into remains unresolved. Exosomes are promising natural nanocarriers for drug delivery that able enter cells with different biological barriers efficiently. In this study, we used exosomes as vehicle target Methods: selectively home fibrotic according animal...
To identify loci useful for species identification and to enhance our understanding of the population structure genetic variability genus Mycobacterium, we conducted a multiple-genome comparison total 27 sequenced genomes in suborder Corynebacterineae (18 from Mycobacterium genus, 7 Corynebacterium 1 each Nocardia Rhodococcus genera). Our study revealed 26 informative Mycobacterium. The sequences these were used phylogenetic analysis infer evolutionary relations 18 mycobacterial genomes....
Abstract Tumor models are important tools for cancer research and drug development. The existing tumor unable to reconstruct the microphysiological processes of immune responses in vivo, thus limiting their applications development immunological drugs immunotherapy. How rapidly accurately construct with a microenvironment an system is critical problem that urgently needs be solved field immunology therapy. In this study, innovatively based on bio-3D printing organ-on-a-chip technology, lung...
The origin recognition complex (ORC) plays a central role in the initiation of DNA replication eukaryotic cells. It interacts with origins chromosomal and recruits additional proteins to form functional complexes. These processes have not been well characterized at biochemical level except case Saccharomyces cerevisiae ORC. We report here expression, purification, initial characterization Schizosaccharomyces pombeORC (SpORC) containing six recombinant subunits. Purified SpORC binds...
This work was carried out to investigate the effect of alcohol drinking on serum LDL. Agarose gel electrophoresis showed that LDL samples from alcoholic patients without serious liver disease were more negatively charged and moved faster toward cathode than nondrinking control subjects. Rabbit antibodies raised by using keyhole limpet hemocyanin modified in vitro 4-hydroxynonenal or acetaldehyde as immunogens reacted strongly with patients' LDL, indicating presence oxidatively epitopes...
During Drosophila metamorphosis, dorsal and ventral wing surfaces adhere, separate, reappose in a paradoxical process involving cell-matrix adhesion, matrix production degradation, long cellular projections. The identity of the intervening matrix, logic behind adhesion-reapposition cycle, role projections are unknown. We find that laminin spots devoid other main basement membrane components mediate adhesion. Through live imaging, we show microtubule-actin cables grow from those adhesion...
The cross-reactivities of four quinolone antibiotics -ofloxacin, norfloxacin, ciprofloxacin, and nalidixic acid - toward the EMIT II Opiates enzyme immunoassay were examined. Drug-free urine was spiked with individual drugs up to 5,000 mg/L. Only ofloxacin showed potential for causing a false-positive opiates screening result (apparent morphine >300 µg/L). method produced positive opiate at an concentration 200 mg/L, 0.16% cross-reactivity. Three hospitalized patients taking therapeutic...
Abstract The construction of reliable preclinical models is crucial for understanding the molecular mechanisms involved in gastric cancer and advancing precision medicine. Currently, existing vitro tumor often do not accurately replicate human environment are unsuitable high‐throughput therapeutic drug screening. In this study, droplet microfluidic technology employed to create novel assembloids by encapsulating patient‐derived xenograft cells patient stromal Gelatin methacryloyl...
Abstract Currently surveillance strategies have inadequate performance for the early detection of hepatocellular carcinoma (HCC). Protein glycosylation is a potential source biomarkers to differentiate between cirrhosis and HCC. We performed comprehensive LC-PRM-MS approach where targeted parallel reaction monitoring (PRM) strategy was coupled powerful LC system study microheterogeneity haptoglobin (Hp) extracted from 15 patients with found that our able identify large number isomeric N-...