- Plant Gene Expression Analysis
- Biochemical Analysis and Sensing Techniques
- Plant Molecular Biology Research
- Cancer Genomics and Diagnostics
- Advanced Biosensing Techniques and Applications
- Gene expression and cancer classification
- Plant biochemistry and biosynthesis
- South Asian Studies and Conflicts
- Phytase and its Applications
- Nuclear Issues and Defense
- South Asian Studies and Diaspora
- Biofuel production and bioconversion
- Natural product bioactivities and synthesis
- Genetic Mapping and Diversity in Plants and Animals
- Plant responses to water stress
- Plant-Microbe Interactions and Immunity
- Global Peace and Security Dynamics
- International Relations and Foreign Policy
- Phytochemical Studies and Bioactivities
- Enzyme Production and Characterization
- Plant Stress Responses and Tolerance
- Plant-derived Lignans Synthesis and Bioactivity
- Medicinal Plants and Neuroprotection
- Advanced Breast Cancer Therapies
- Populism, Right-Wing Movements
Institute of Himalayan Bioresource Technology
2015-2024
Academy of Scientific and Innovative Research
2019-2024
Dr. Yashwant Singh Parmar University of Horticulture and Forestry
2024
Sardar Vallabhbhai Patel University of Agriculture & Technology
2024
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2022
Polish Academy of Sciences
2022
Indira Gandhi Medical College
2021
G.B. Pant Institute of Himalayan Environment and Development
2020
Council of Scientific and Industrial Research
2019
Bellingham Technical College
2017
β-Glucosidases are diverse group of enzymes with great functional importance to biological systems. These grouped in multiple glycoside hydrolase families based on their catalytic and sequence characteristics. Most studies carried out β-glucosidases focused industrial applications rather than endogenous function the target organisms. performed many functions bacteria as they components large complexes called cellulosomes responsible for hydrolysis short chain oligosaccharides cellobiose. In...
Abstract Trillium govanianum , an endangered medicinal herb native to the Himalaya, is less studied at molecular level due non-availability of genomic resources. To facilitate basic understanding key genes and regulatory mechanism pharmaceutically important biosynthesis pathways, first spatial transcriptome sequencing T. was performed. 151,622,376 (~11.5 Gb) high quality reads obtained using paired-end Illumina were de novo assembled into 69,174 transcripts. Functional annotation with...
Abstract Stevia is a natural source of commercially important steviol glycosides (SGs), which share biosynthesis route with gibberellic acids (GAs) through plastidal MEP and cytosolic MVA pathways. Ontogeny-dependent deviation in SGs one the key factor for global cultivation Stevia, has not been studied at transcriptional level. To dissect underlying molecular mechanism, we followed transcriptome sequencing approach generated more than 100 million reads. Annotation 41,262 de novo assembled...
Tea is popular health beverage consumed by millions of people worldwide. Drought among the acute abiotic stress severely affecting tea cultivation, globally. In current study, transcriptome sequencing four diverse genotypes with inherent contrasting genetic response to drought (tolerant & sensitive) generated more than 140 million reads. De novo and reference-based assembly functional annotation 67,093 transcripts multifarious public protein databases yielded 54,484 (78.2%) significant...
Abstract Considering the major source of plant-derived low/non-calorie steviol glycosides (SGs), comprehensive physiological, biochemical, and deep transcriptional investigations were conducted to explicit deeper insight into multiple abiotic stress responses in Stevia rebaudiana . The physiological indicators including photosynthesis, chlorophyll, relative water content, shoot growth, electrolyte leakage, SG biosynthesis negatively impacted under drought (DS), followed by salinity (SS)...
The present study investigates the life cycle and reproductive biology of Callosobruchus maculatus, a major pest stored pulses, with focus on its development chickpea (Cicer arietinum). experiment was conducted under controlled laboratory conditions at 30 ± 1°C. results revealed that total developmental period, from egg to adult emergence, averaged 35.79 1.81 days. incubation period lasted 5.93 0.63 days, while larval pupal stages combined took 27.79 0.83 sex ratio recorded 1:0.93 (male...
Summary Stevia rebaudiana Bertoni is popular source of plant‐derived low/no‐calorie natural sweeteners (LNCSs), collectively known as steviol glycosides (SGs). Nevertheless, genetic predisposition for targeted biosynthesis SGs complex due to multi‐substrate functionality key uridine diphosphate glycosyltransferases (UGTs). Here, we created a high‐quality monoploid assembly 1.34 Gb with N50 value 110 Mb, 55 551 predicted protein‐coding genes, and ~80% repetitive regions in Rebaudioside‐A...
Histidine acid phytases (HAPhy) are widely distributed enzymes among bacteria, fungi, plants, and some animal tissues. They have a significant role as an feed enzyme in the solubilization of insoluble phosphates minerals present form phytic complex. A set 50 reference protein sequences representing HAPhy were retrieved from NCBI database characterized for various biochemical properties, multiple sequence alignment (MSA), homology search, phylogenetic analysis, motifs, superfamily search. MSA...
Angelica glauca Edgew, which is an endangered medicinal and aromatic herb, a rich source of numerous industrially important bioactive metabolites, including terpenoids, phenolics, phthalides. Nevertheless, genomic interventions for the sustainable utilization restoration its genetic resources are greatly offset due to scarcity key regulators underlying specialized metabolism. To unravel global atlas metabolism, first spatial transcriptome sequencing leaf, stem, root generated 109 million...
β -Propeller phytases (BPPhy) are widely distributed in nature and play a major role phytate-phosphorus cycling. In the present study, BPPhy gene from Bacillus licheniformis strain was expressed E. coli with phytase activity of 1.15 U/mL specific 0.92 U/mg proteins. The enzyme represented full length ORF “PhyPB13” 381 amino acid residues differs by 3 closest similar existing sequences. PhyPB13 sequence characterized silico using various bioinformatic tools to better understand structural,...
Inula racemosa Hook. f. (Pushkarmula), a perennial Himalayan herb known for its aromatic and phytopharmaceutical attributes, is not yet explored at genomic/transcriptomic scale. In this study, efforts were made to unveil the global transcriptional atlas underlying organ-specific specialized metabolite biosynthesis by integrating RNA-Seq analysis of 433 million sequenced reads with phytochemical leaf, stem, root tissues. Overall, 7242 83,772 assembled nonredundant unigenes identified...
The pathogen Helminthosporium oryzae was subjected to different cultural conditions viz., media, temperature, pH and nitrogen sources under in vitro conditions. supreme radial progress of Czapeck Agar, OMA & PDA recorded highest with mean growth followed by Richard’s agar (88 mm), Water agar. colony showed up as thick, leathery, slightly elevated, abundant mycelia brown-coloured conidia on this medium. Its colour ranged from greyish-white dark brown. When exposed constant light, the...