RNA-binding proteins (RBPs) have essential roles in determining the fate of RNA from synthesis to decay and been studied on a protein-by-protein basis, or computationally based number well-characterised domains. Recently, high-throughput methods enabled capture mammalian proteomes. To gain insight into role Arabidopsis thaliana RBPs at systems level, we employed interactome techniques using cells different ecotypes grown cultures leaves. In vivo UV-crosslinking RBPs, oligo(dT) mass...

Increasing structural and biochemical evidence suggests that post-translational methionine oxidation of proteins is not just a result cellular damage but may provide the cell with information on oxidative status. In addition, residues in key regulatory proteins, such as calmodulin, does influence homeostasis. Previous findings also indicate signaling molecules have role stress responses since these specific modifications can turn change biological activities proteins.Here we use tandem mass...

Corals and their endosymbiotic dinoflagellates of the genus Symbiodinium have a fragile relationship that breaks down under heat stress, an event known as bleaching. However, many coral species adapted to high temperature environments such Red Sea (RS). To investigate mechanisms underlying adaptation in zooxanthellate cnidarians we compared transcriptome- proteome-wide stress response (24 h at 32°C) three strains model organism Aiptasia pallida from regions with differing profiles; North...

RNA-binding proteins (RBPs) are increasingly recognized as regulatory component of post-transcriptional gene expression. RBPs interact with mRNAs via domains and these interactions affect RNA availability for translation, stability turn-over thus affecting both protein expression essential developmental stimulus specific responses. Here we investigate the effect severe drought stress on proteome to gain insights into mechanisms that govern responses at systems level. Label-free mass...

Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H 2 O ) induces rapid net K + ‐efflux and Ca 2+ ‐influx Arabidopsis roots. Pre‐treatment with either 10 μM cAMP or cGMP for 1 24 h does significantly reduce ‐leakage ‐influx, the case of ‐fluxes, cell permeant cyclic more effective. We also examined effect 8‐Br‐cGMP on microsomal proteome noted a specific increase proteins role responses ion transport, suggesting is sufficient to directly...

Xanthomonas citri subsp.citri (Xcc) is a bacterial pathogen that causes citrus canker in susceptible Citrus spp.The Xcc genome contains genes encoding enzymes from three separate pathways of trehalose biosynthesis.Expression trehalose-6-phosphate synthase (otsA) and phosphatase (otsB) was highly induced during development, suggesting the two-step pathway biosynthesis via has function pathogenesis.This eliminated bacterium by deletion otsA gene.The resulting XccΔotsA mutant produced less than...

Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein system (T3SS). The role T3SS in pathogenesis is well established but whether this involved multicellular processes, such as biofilm formation has not been elucidated. Here, phytopathogen Xanthomonas citri subsp. (X. citri) was used model to gain further insights about formation.The capacity different X. mutants compared wild type strain and it observed that necessary for...

Summary The brassinosteroid receptor insensitive 1 ( BRI 1) is a member of the leucine‐rich repeat receptor‐like kinase family. intracellular domain an active and also encapsulates guanylate cyclase catalytic centre. Using liquid chromatography tandem mass spectrometry, we confirmed that recombinant cytoplasmic generates pmol amounts cGMP per μg protein with preference for magnesium over manganese as co‐factor. Importantly, functional essential optimal generation. Therefore, activity...

Indoor horticulture offers a sensible solution for sustainable food production and is becoming increasingly widespread. However, it incurs high energy cost due to the use of artificial lighting such as high-pressure sodium lamps, fluorescent light or increasingly, light-emitting diodes (LEDs). The efficiency quality currently available horticultural suboptimal, therefore less than ideal cost-effective large-scale plant production. Here, we demonstrate high-powered single-wavelength lasers...

Abstract Background Xanthomonas axonopodis pv. citri ( X . a ) causes citrus canker that can result in defoliation and premature fruit drop with significant production losses worldwide. Biofilm formation is an important process bacterial pathogens several lines of evidence suggest this requirement to achieve maximal virulence since it has major role host interactions. In study, proteomics was used gain further insights into the functions biofilms. Results order identify differentially...

Date palm (Phoenix dactylifera) is an economically important fruit tree in the Middle East and North Africa characterized by large cultivar diversity, making it a good model for studies on development other traits. Here gel comparative proteomics combined with tandem mass spectrometry were used to study date ripening. Total proteins extracted using phenol-based protocol. A total of 189 protein spots differentially regulated (p≤0.05). The identified classified into 14 functional categories....

The second messenger 3',5'-cyclic adenosine monophosphate (cAMP) is increasingly recognized as having many different roles in plant responses to environmental stimuli. To gain further insights into these roles, Arabidopsis thaliana cell suspension culture was treated with 100 nM of permeant 8-bromo-cAMP for 5 or 10 min. Here, applying mass spectrometry and comparative proteomics, 20 proteins were identified differentially expressed we noted a specific bias role abiotic stress, particularly...

Post-transcriptional regulation has far-reaching implications on the fate of RNAs. It is gaining increasing momentum as a critical component in adjusting global cellular transcript levels during development and response to environmental stresses. In this process, RNA-binding proteins (RBPs) are indispensable chaperones that naturally bind RNA via one or multiple globular domains (RBDs) changing function bound Despite technical challenges faced plants large-scale studies, several hundreds...

The spliceosome processes RNAs from a pre-RNA state to mature mRNA thereby influencing RNA availability for translation, localization, and turnover. It consists of complex structures containing RNA-binding proteins (RBPs) essential post-transcriptional gene expression control. Here we investigate the dynamic modifications spliceosomal RBPs under stress in particular drought stress. We do so by interactome capture Arabidopsis thaliana using label free quantitation. This approach identified 44...

Phytosulfokines (PSKs) are plant peptide hormones that co-regulate growth, differentiation and defense responses. PSKs signal through a plasma membrane localized leucine-rich repeat receptor-like kinase (phytosulfokine receptor 1, PSKR1) also contains functional cytosolic guanylate cyclase with its catalytic center embedded within the domain. To functionally characterize this novel type of overlapping dual function, we investigated phosphorylation PSKR1 in vitro Tandem mass spectrometry...

Signal modulation is important for the growth and development of plants this process mediated by a number factors including physiological regulators their associated signal transduction pathways. Protein kinases play central role in signaling, those involving pathogen response mechanisms. We previously demonstrated an active guanylate cyclase (GC) catalytic center brassinosteroid insensitive receptor (AtBRI1) within intracellular kinase domain resulting dual enzymatic activity. Here we...

In plants, structural and physiological evidence has suggested the presence of biologically active natriuretic peptides (PNPs). PNPs are secreted into apoplast, systemically mobile elicit a range responses signaling via cGMP. The PNP-dependent include tissue specific modifications cation transport changes in stomatal conductance photosynthetic rate. PNP also critical role host defense responses. Surprisingly, PNP-homologs produced by several plant pathogens during colonization suppressing...