A5038474417- Monoclonal and Polyclonal Antibodies Research
- Click Chemistry and Applications
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Chemical Synthesis and Analysis
- Immune Cell Function and Interaction
- Lung Cancer Treatments and Mutations
- IL-33, ST2, and ILC Pathways
- HER2/EGFR in Cancer Research
- Pancreatic function and diabetes
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Clostridium difficile and Clostridium perfringens research
- Wnt/β-catenin signaling in development and cancer
- Surgical Simulation and Training
- Advanced Biosensing Techniques and Applications
- Cell Image Analysis Techniques
- Food Allergy and Anaphylaxis Research
- Colorectal Cancer Treatments and Studies
- Synthesis and Biological Evaluation
- Advanced Fluorescence Microscopy Techniques
- Neuroendocrine Tumor Research Advances
- Radiomics and Machine Learning in Medical Imaging
- PI3K/AKT/mTOR signaling in cancer
NYU Langone Health
2022-2025
New York University
2021-2025
University School
2024
NYU Langone’s Laura and Isaac Perlmutter Cancer Center
2021
Center for Systems Biology
2008-2014
Massachusetts General Hospital
2006-2014
Harvard University Press
2011
Harvard University
2006-2010
Gordon Center for Medical Imaging
2006-2008
Athinoula A. Martinos Center for Biomedical Imaging
2008
Glowing tags: a series of activatable ("turn-on") tetrazine-conjugated fluorescent probes was developed, which react rapidly in an inverse-electron-demand [4+2] cycloaddition with strained dienophiles such as trans-cyclooctene, thereby strongly increasing the fluorescence intensity. The novel turn-on were applied for intracellular live-cell imaging microtubuli-binding trans-cyclooctene modified taxol.
A label for six, please! fluorescent tetrazine derivative was used to image trans-cyclooctene-modified affinity ligands on live cancer cells through a bioorthogonal cycloaddition with reaction rate of approximately 6000±200 M−1 s−1 in serum at 37 °C (see scheme). To maximize the fluorescence signal, up six trans-cyclooctene moieties were attached antibody pretarget labeling.
Given mounting evidence of the importance gut-microbiota/immune-cell interactions in immune homeostasis and responsiveness, surprisingly little is known about leukocyte movements to, especially from, gut. We address this topic a minimally perturbant manner using Kaede transgenic mice, which universally express photoconvertible fluorescent reporter. Transcutaneous exposure cervical lymph nodes to violet light permitted punctual tagging cells specifically therein, subsequent monitoring their...
Leuchtmarker: Eine Serie von aktivierbaren ("einschaltbaren") Tetrazin-konjugierten Fluoreszenzsonden wurde entwickelt, die durch schnelle [4+2]-Cycloadditionen mit inversem Elektronenbedarf an sterisch gespannte Dienophile wie trans-Cycloocten addieren, wodurch es zu einem drastischen Anstieg der Fluoreszenzaktivität kommt (siehe Bild). Die neuen "Einschalt"-Sonden wurden für intrazelluläre Bildgebung lebender Zellen Mikrotubuli bindenden trans-Cycloocten-modifizierten Taxol verwendet....
The hallmark of type 1 diabetes is autoimmune destruction the insulin-producing β-cells pancreatic islets. Autoimmune has been difficult to study or treat because it not usually diagnosed until substantial β-cell loss already occurred. Imaging agents that permit noninvasive visualization changes in mass remain a high-priority goal. We report on development and testing near-infrared fluorescent imaging agent. Based amino acid sequence exendin-4, we created neopeptide via introduction an...
There is clinical equipoise for COVID-19 convalescent plasma (CCP) use in patients hospitalized with COVID-19.To determine the safety and efficacy of CCP compared placebo receiving noninvasive supplemental oxygen.CONTAIN COVID-19, a randomized, double-blind, placebo-controlled trial adults was conducted at 21 US hospitals from April 17, 2020, to March 15, 2021. The enrolled 941 participants who were 3 or less days presented 7 after symptom onset required oxygen supplementation.A unit...
Abstract The immune response to SARS-CoV-2 antigen after infection or vaccination is defined by the durable production of antibodies and T cells. Population-based monitoring typically focuses on antibody titer, but there a need for improved characterization quantification cell responses. Here, we used multimodal sequencing technologies perform longitudinal analysis circulating human leukocytes collected before immunization with mRNA vaccine BNT162b2. Our data indicated distinct...
Current therapy for lung cancer involves multimodality therapies. However, many patients are either refractory to or develop drug resistance. KRAS and epidermal growth factor receptor (EGFR) mutations represent some of the most common in cancer, studies have shown importance these both carcinogenesis chemoresistance. Genetically engineered murine models mutant EGFR been developed that more accurately recapitulate human cancer. Recently, using cell-based experiments, we showed platinum-based...
Deutlich sichtbar: Ein fluoreszierendes Tetrazinderivat wurde verwendet, um mit trans-Cycloocten modifizierte Affinitätsliganden auf lebenden Krebszellen abzubilden. Die bioorthogonale Cycloaddition läuft bei 37 °C in Serum einer Reaktionsgeschwindigkeit von ungefähr (6000±200) M−1 s−1 ab (siehe Schema). Um das Fluoreszenzsignal zu maximieren, wurden bis sechs trans-Cycloocten-Gruppen an die zur ortsspezifischen Bindung Zelloberfläche eingesetzten Antikörper geknüpft. Detailed facts of...
Eosinophils are multifunctional leukocytes that degrade and remodel tissue extracellular matrix through production of proteolytic enzymes, release proinflammatory factors to initiate propagate inflammatory responses, direct activation mucus secretion smooth muscle cell constriction. Thus, eosinophils central effector cells during allergic airway inflammation an important clinical therapeutic target. Here we describe the use injectable MMP-targeted optical sensor specifically quantitatively...
Purpose To use near-infrared (NIR) optical imaging to assess the therapeutic susceptibility and drug dosing of orthotopic human breast cancers implanted in mice treated with molecularly targeted therapy. Materials Methods This study was approved by institutional animal care committee. Imaging probes were synthesized conjugating epidermal growth factor receptor type 2 (HER2)-specific antibody trastuzumab fluorescent dyes. In vitro probe binding assessed flow cytometry. HER2-normal...
Abstract A number of exendin derivatives have been developed to target glucagon‐like peptide 1 (GLP‐1) receptors on beta cells in vivo. Modifications analogues shown significant effects pharmacokinetics and, as such, used develop a variety therapeutic compounds. Here, we show that an exendin‐4, modified at position 12 with cysteine conjugated tetrazine, can be labeled 18 F‐ trans ‐cyclooctene and converted into PET imaging agent high yields good selectivity. The accumulates vivo has...
Abstract The gastrointestinal tract is continuously exposed to foreign antigens in food and commensal microbes with potential induce adaptive immune responses. Peripherally induced T regulatory (pTreg) cells are essential for mitigating inflammatory responses these agents 1–4 . While RORγt + antigen-presenting (RORγt-APCs) were shown program gut microbiota-specific pTreg 5–7 , their definition remains incomplete, the APC responsible tolerance has remained elusive. Here, we identify a...
Purpose: To prospectively evaluate an optical imaging system designed to perform quantitative, intravital catheter-based of fluorescent molecular probes. Materials and Methods: This study was performed according a protocol approved by the institutional animal care committee. A fiberoptic catheter developed implement normalization algorithm for real-time quantitative near-infrared (NIR) imaging. The validated with in vitro fluorochrome phantoms vivo fluorescence measurements obtained tumors...
The genetic basis of pancreatic ductal adenocarcinoma, which constitutes the most common type malignancy, involves sequential activation oncogenes and inactivation tumor suppressor genes. Among pivotal alterations are Ki-RAS oncogene p53 gene inactivation. We explain that combination these events facilitates carcinogenesis as revealed in novel three-dimensional cell (spheroid cyst) culture vivo subcutaneous orthotopic xenotransplantation models. N-cadherin, a member classic cadherins...
Green fluorescent protein (GFP) has been used for cell tracking and imaging gene expression in superficial or surgically exposed structures. However, vivo murine is often limited by several factors, including scatter attenuation with depth overlapping autofluorescence. The autofluorescence signals have spectral profiles that are markedly different from the GFP emission profile. use of allows separation quantitation these contributions to total fluorescence signal seen weighting known pure...
Cancer cells interact with surrounding stromal fibroblasts during tumorigenesis, but the complex molecular rules that govern these interactions remain poorly understood thus hindering development of therapeutic strategies to target cancer stroma. We have taken a mathematical approach begin defining by performing first large-scale quantitative analysis fibroblast effects on cell proliferation across more than four hundred heterotypic line pairings. Systems-level modeling this dataset using...
Abstract Food antigens elicit immune tolerance through the action of regulatory T cells (Tregs) in intestine. Although that trigger common food allergies are known, epitopes mediate to most foods have not been described. Here, we identified murine cell receptors specific for maize, wheat, and soy, used expression cloning de-orphan their cognate epitopes. All derive from seed storage proteins resistant degradation abundant edible portion plant. Multiple unrelated clones were an epitope at...
To address the need for a clinically applicable intravital optical imaging system, we developed new hardware and software framework. We demonstrate its utility by applying it to an endoscope-based white light fluorescent system. The capabilities include acquisition visualization algorithms that perform registration, segmentation, histogram-based autoexposure of two channels (full-spectrum near-infrared fluorescence), all in real time. Data are processed saved as 12-bit files, matching...
Abstract New cancer therapies are increasingly molecular‐pathway specific. The evaluation of these novel would be greatly facilitated by the development noninvasive methods to assess multiple tumor cellular and molecular parameters. Using fluorescent probes specific for HER2/ neu (AF750‐trastuzumab) apoptosis (Cy5.5‐Annexin), we demonstrate a multichannel near infrared imaging approach that yields accurate early assessment treatment susceptibility, drug target inhibition response during...
The human immune response to SARS-CoV-2 antigen after infection or vaccination is defined by the durable production of antibodies and T cells. Population-based monitoring typically focuses on antibody titer, but there a need for improved characterization quantification cell responses. Here, we utilize multimodal sequencing technologies perform longitudinal analysis circulating leukocytes collected before BNT162b2 immunization. Our data reveal distinct subpopulations CD8