A5044731106- RNA Research and Splicing
- Neurogenesis and neuroplasticity mechanisms
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- Power Systems and Technologies
- Muscle Physiology and Disorders
- bioluminescence and chemiluminescence research
- Adipose Tissue and Metabolism
- Fetal and Pediatric Neurological Disorders
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Muscle metabolism and nutrition
- Hippo pathway signaling and YAP/TAZ
- Medical Imaging Techniques and Applications
- Herpesvirus Infections and Treatments
- High-Voltage Power Transmission Systems
- Cancer, Hypoxia, and Metabolism
- Immunotherapy and Immune Responses
- Poxvirus research and outbreaks
- Signaling Pathways in Disease
- Plant responses to water stress
- RNA regulation and disease
- Glioma Diagnosis and Treatment
- Neonatal and fetal brain pathology
- Adipokines, Inflammation, and Metabolic Diseases
Sungkyunkwan University
2002-2019
Samsung Medical Center
2002-2003
Optical luciferase gene imaging is emerging as a method to monitor expression in small animals. However, there concern over how regional availability of exogenously administered substrate may affect photon emission. We thus synthesized [125I]iodo-D-luciferin, which demonstrated characteristics for firefly luciferase, and investigated its cell uptake kinetics vivo biodistribution. Luminescence assays luc transduced cells confirmed linear decline emitted light units with decreasing luciferin...
Smallpox, caused by the variola virus belonging to genus Orthopoxvirus, is an acute contagious disease that killed 300 million people in 20th century. Since it was declared be eradicated and national immunization program against stopped, has become a prospective bio-weapon. It necessary develop safe vaccine protects from terrorism using this biological weapon can administered immunocompromised people. Our previous study reported on development of attenuated smallpox (KVAC103). This evaluated...
Despite the high incidence of severe defects in central nervous system caused by human cytomegalovirus (HCMV) congenital infection, mechanism HCMV neuropathogenesis and roles individual viral genes have not yet been fully determined. In this study, we show that immediate-early 2 (IE2) protein may play a key role HCMV-caused neurodevelopmental disorders. IE2-transduced neural progenitor cells gave rise to neurospheres with lower frequency produced smaller than control vitro, indicating...
Creatine is a nitrogenous organic acid known to function in adenosine triphosphate (ATP) metabolism. Recent evidence indicates that creatine regulates the differentiation of mesenchymal stem cells (MSCs) processes such as osteogenesis and myogenesis. In this study, we show also has negative regulatory effect on fat cell formation. inhibits accumulation cytoplasmic triglycerides dose-dependent manner irrespective adipogenic models used, including C3H10T1/2 MSC line, 3T3-L1 preadipocytes,...
While 18F-FDG labelling of monocytes would allow in vivo trafficking with positron emission tomography (PET), present methods suffer from poor retention radioactivity. We investigated the feasibility utilizing insulin for improved [18F]fluorodeoxyglucose (18F-FDG) labelling. Separated human and lymphocytes were labelled or without 3 h pre-incubation. Insulin had no effect on lymphocyte (21.4±0.8% vs 20.8±1.1% efficiency, P = NS). However, monocytes, pre-incubation led to a 169±9% increase...
Notch has a broad range of regulatory functions in many developmental processes, including hematopoiesis, neurogenesis, and angiogenesis. several key functional regions such as the RBP-Jκ/CBF1 association module (RAM) domain, nuclear localization signals (NLS), ankyrin (ANK) repeats. However, previous reports assessing level importance these domains signaling pathway are controversial. In this study, we have assessed contribution each domain to regulation mammalian neural stem cells vivo...
Transactivation response element RNA-binding protein (TRBP) is known to play important roles in human immunodeficiency virus (HIV) replication and microRNA biogenesis. However, recent studies implicate TRBP a variety of biological processes as mediator for cross-talk between signal transduction pathways. Here, we provide the first evidence that required efficient neurosphere formation, expression neural stem cell markers Notch target genes primary progenitor cells vitro. Consistent with...
CTUSBDU +FKV QPXFS TZTUFN JT DPOOFDUFE UP UIF NBJOMBOE UISPVHI )7%$ BOE SFDFJWFT BCPVU IBMG PG SFRVJSFE FMFDUSJDJUZ MJOFT BMSFBEZ FYQFSJFODFE CMBDL PVU XIFO B HFOFSBUPS USJQQFE BU NPNFOU MJOF GBVMUT #VU 6'3 PQFSBUFE BT XBT FYQFDUFE GBVMU PDDVSSFE UIBU UJNF T QFOFUSBUJPO MFWFM XJOE UVSCJOFT JODSFBTFT JU TFU BHBJO DPOTJEFSJOH JOUFSNJUUFOU UVSCJO PVUQVUT *O UIJT QBQFS XF BEESFTT OFX XBZ TFUUJOH DPNQVUFS TJNVMBUJPO ,FZ 8PSET 8JOE -PBE TIFEEJOH %FQBSUNFOU &MFDUSJDBM &MFDUSPOJD...