A5053842742- S100 Proteins and Annexins
- Parathyroid Disorders and Treatments
- Bone health and treatments
- Vitamin D Research Studies
- Cell Adhesion Molecules Research
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- Erythrocyte Function and Pathophysiology
- Radiopharmaceutical Chemistry and Applications
- Protease and Inhibitor Mechanisms
- Inflammatory Bowel Disease
- TGF-β signaling in diseases
- Thyroid Disorders and Treatments
- Computational Drug Discovery Methods
- RNA modifications and cancer
- Microscopic Colitis
- Metabolomics and Mass Spectrometry Studies
- Folate and B Vitamins Research
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Pancreatic function and diabetes
- Genomics, phytochemicals, and oxidative stress
- Connexins and lens biology
- Parasitic Diseases Research and Treatment
- Cancer, Stress, Anesthesia, and Immune Response
Meharry Medical College
2016-2025
Tennessee Oncology
2018
University of Toronto
2007-2012
Canada Research Chairs
2012
University of New Brunswick
2012
Uppsala University
2002-2007
University of Buea
1997
Université de Yaoundé I
1990
Exosomes are nano-vesicles secreted by a wide range of mammalian cell types. These vesicles abundant in serum and other extracellular fluids contain large repertoire proteins, mRNA microRNA. have been implicated to communication, the transfer infectious agents, neurodegenerative diseases as well tumor progression. However, precise mechanisms which they internalized and/or remain poorly understood. In order follow their release uptake breast cells real time, cell-derived exosomes were tagged...
Minichromosome maintenance (MCM) complex replicative helicase complexes play essential roles in DNA replication all eukaryotes. Using a tandem affinity purification-tagging approach human cells, we discovered form of the MCM that contains previously unstudied protein, binding protein (MCM-BP). MCM-BP is conserved multicellular eukaryotes and shares limited homology with proteins. formed MCM3 to MCM7, which excluded MCM2; and, conversely, hexameric MCM2 MCM7 lacked MCM-BP, indicating can...
Our goal in this study was to define the mechanisms by which fetuin‐A mediates adhesion of tumor cells. The data show that absence fetuin‐A, detached cells secrete exosomes contain most known exosomal associated proteins but lack capacity mediate cellular adhesion. In presence exosomes, contain, addition other proteins, plasminogen and histones. These cell spreading. Plasminogen is a participant novel mechanism. suggest these play role progression.
The expression of annexin A6 (AnxA6) in AnxA6-deficient non-invasive tumor cells has been shown to terminate epidermal growth factor receptor (EGFR) activation and downstream signaling. However, as a scaffolding protein, AnxA6 may stabilize activated cell-surface receptors promote cellular processes such cell motility invasiveness. In this study, we investigated the contribution activity EGFR invasive breast cancer examined whether status influences response these EGFR-targeted tyrosine...
The mechanisms by which exosomes (nano‐vesicular messengers of cells) are taken up recipient cells poorly understood. We hypothesized that histones associated with these nanoparticles the ligands facilitate their interaction cell surface syndecan‐4 ( SDC 4) to mediate uptake. show incubation fetuin‐A (exosome‐associated proteins) and mediates uptake normally not endocytosed. Similarly, hydroxyapatite‐nanoparticles incubated FNH ) internalized tumor cells, while alone FN not. , both move...
The aberrant expression of α defensin 5 (DEFA5) protein in colonic inflammatory bowel diseases (IBDs) underlies the distinct pathogenesis Crohn's colitis (CC). It can serve as a biomarker for differentiating CC from Ulcerative (UC), particularly Indeterminate (IC) cases into UC and CC. We evaluated specificity commercially available anti-DEFA5 antibodies, emphasizing need to further validate their appropriateness given application highlighting necessity novel antibodies. established two mice...
The present studies were undertaken to address the innovative role of fetuin-A in growth and invasion potential a triple negative breast cancer (TNBC) cell line, MDA-MB-468. Basal like TNBC that express high levels ectopic have poorer prognosis for patients compared those low protein. We overexpressed MDA-MB-468 then determined invasive overexpressing cells vs controls transfected with empty vector. also adhesion presence only serum free medium complete medium. Our data suggest...
Breast cancer (BC) patients with late-stage and/or rapidly growing tumors are prone to develop high serum calcium levels which have been shown be associated larger and aggressive breast in post premenopausal women respectively. Given the pivotal role of sensing receptor (CaSR) homeostasis, we evaluated whether polymorphisms CASR gene at rs1801725 rs1801726 SNPs exon 7, circulating African American Caucasian control subjects BC cases. In this retrospective case-control study, assessed mean...
Inability to distinguish Crohn's colitis from ulcerative leads the diagnosis of indeterminate colitis. This greatly effects medical and surgical care patient because treatments for two diseases vary. Approximately 30 percent inflammatory bowel disease patients cannot be accurately diagnosed, increasing their risk inappropriate treatment. We sought determine whether transcriptomic patterns could used develop diagnostic biomarker(s) delineate more accurately. Four groups were assessed via...
Abstract The epidermal growth factor receptor (EGFR) is a major oncogene in triple-negative breast cancer (TNBC), but the use of EGFR-targeted tyrosine kinase inhibitors (TKI) and therapeutic monoclonal antibodies associated with poor response acquired resistance. Understanding basis for resistance to these drugs identifying biomarkers monitor ensuing remain challenge. We previously showed that reduced expression annexin A6 (AnxA6), calcium-dependent membrane-binding tumor suppressor, not...
Megalin is an integral membrane receptor belonging to the low-density lipoprotein family. In addition its role as endocytotic receptor, megalin has also been proposed have signalling functions. Using interaction cloning in yeast, we identified membrane-associated guanylate kinase family member postsynaptic density-95 (PSD-95) partner for megalin. PSD-95 and a truncated version of were co-immunoprecipitated from HEK-293 cell lysates overexpressing two proteins, which confirmed interaction....
Mini-chromosome maintenance complex-binding protein (MCM-BP) was discovered as a that is strongly associated with human MCM proteins, known to be crucial for DNA replication in providing helicase activity. The Xenopus MCM-BP homologue appears play role unloading complexes from chromatin after synthesis; however, the importance of and its functional contribution cells has been unclear. Here we show depletion by sustained expression short hairpin RNA (shRNA) results highly abnormal nuclear...
Abstract Glioblastomas (high‐grade astrocytomas) are highly aggressive brain tumors with poor prognosis and limited treatment options. In the present studies, we have defined role of fetuin‐A, a liver‐derived multifunctional serum protein, in growth an established glioblastoma cell line, LN 229. We hereby demonstrate that these cells synthesize ectopic fetuin‐A which supports their culture absence serum. demonstrated panel tissue microarray ( TMA ) glioblastomas also express fetuin‐A....
Physiological changes such as hypoxia in the tumor microenvironment (TME) endow cancer cells with malignant properties, leading to recurrence and rapid progression. Here, we assessed effect of (1% Oxygen) on suppressor Annexin A6 (AnxA6) response triple-negative breast (TNBC) epidermal growth factor receptor (EGFR) androgen (AR) targeted therapies. We demonstrate that brief exposure TNBC (within 24 h) is associated down regulation AnxA6 while > h cell type dependently stimulated...