A5036435246- SARS-CoV-2 and COVID-19 Research
- Blood groups and transfusion
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Complement system in diseases
- Chemical Synthesis and Analysis
- Alzheimer's disease research and treatments
- vaccines and immunoinformatics approaches
- Ubiquitin and proteasome pathways
- Supramolecular Self-Assembly in Materials
- Diabetes and associated disorders
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Click Chemistry and Applications
- Lipid Membrane Structure and Behavior
- Viral Infectious Diseases and Gene Expression in Insects
- IgG4-Related and Inflammatory Diseases
- RNA and protein synthesis mechanisms
- Adenosine and Purinergic Signaling
The University of Texas at Austin
2020-2024
Memorial Sloan Kettering Cancer Center
2014-2020
Cornell University
2014-2020
The molecular composition and binding epitopes of the immunoglobulin G (IgG) antibodies that circulate in blood plasma after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection are unknown. Proteomic deconvolution IgG repertoire to spike glycoprotein convalescent subjects revealed response is directed predominantly (>80%) against residing outside receptor domain (RBD). In one subject, just four lineages accounted for 93.5% response, including an amino (N)-terminal...
We describe the molecular-level composition of polyclonal immunoglobulin G (IgG) anti-spike antibodies from ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, vaccination, or their combination ("hybrid immunity") at monoclonal resolution. Infection primarily triggers S2/N-terminal domain (NTD)-reactive antibodies, whereas vaccination mainly induces anti-receptor-binding (RBD) antibodies. This imprint persists after secondary exposures wherein >60% ensuing...
SUMMARY Although humoral immunity is essential for control of SARS-CoV-2, the molecular composition, binding epitopes and effector functions immunoglobulin G (IgG) antibodies that circulate in blood plasma following infection are unknown. Proteomic deconvolution circulating IgG repertoire (Ig-Seq 1 ) to spike ectodomain (S-ECD 2 four convalescent study subjects revealed response oligoclonal directed predominantly (>80%) S-ECD lie outside receptor domain (RBD). When comparing either RBD,...
Here we describe YESS 2.0, a highly versatile version of the yeast endoplasmic sequestration screening (YESS) system suitable for engineering and characterizing protein/peptide modifying enzymes such as proteases with desired new activities. By incorporating features that modulate gene transcription well substrate enzyme spatial sequestration, 2.0 achieves significantly higher operational dynamic range compared original YESS. To showcase advantages improved an already efficient TEV protease...
We used plasma IgG proteomics to study the molecular composition and temporal durability of polyclonal antibodies triggered by ancestral SARS-CoV-2 infection, vaccination, or their combination ("hybrid immunity"). Infection, whether primary post-vaccination, mainly an anti-spike antibody response S2 domain, while vaccination predominantly induced anti-RBD antibodies. Immunological imprinting persisted after a secondary (hybrid) exposure, with >60% ensuing serological originating from initial...
Significance The bromodomain–histone binding motif has been shown to read epigenetic marks on chromatin, and proteins with a bromodomain can have powerful effects gene expression transcriptional regulation. ATAD2 is one such protein linked wide variety of cancers correlating strongly poor tumor prognosis. We developed an efficient chemical synthesis the region using modern ligation methods assemble peptide fragments. Lessons learned in this synthetic exploration include number potentially...
γ-Secretase is a multisubunit complex that catalyzes intramembranous cleavage of transmembrane proteins. The lipid environment forms membrane microdomains serve as spatio-temporal platforms for proteins to function properly. Despite substantial advances in the regulation γ-secretase, effect local microenvironment on γ-secretase poorly understood. Here, we characterized and quantified partitioning its substrates, amyloid precursor protein (APP) Notch, into bilayers using solid-supported model...
Synthesis and use of benzimidazole activity-based probes to validate target labeling identify novel binding partners.
Abstract Extensive efforts have been made to study the human immune response following SARS-CoV-2 infection. Recent work by our lab provided insights into molecular-level composition of circulating IgG antibody repertoire evoked primary infection and revealed key properties spike-directed antibodies epitopes they target in convalescent plasma (Voss et al., Science 2021). We showed that mild is directed outside RBD primarily S2 domain spike glycoprotein. The continual emergence globally...