Introduction Entomopathogenic fungi (EPF) can colonize and establish symbiotic relationships with plants as endophytes. Recently, EPF have been reported to suppress plant pathogens induce resistance diseases. However, the potential mechanisms via which endophytes control major diseases in situ remain largely unknown. Methods Pot field experiments were conducted investigate an EPF, Beauveria bassiana , colonizes tomato, under Botrytis cinerea infection stress. B. blastospores inoculated into...

The economic and environmental value of honeybees has been severely challenged in recent years by the collapse their colonies worldwide, often caused outbreaks infectious diseases. However, our understanding diversity, prevalence, transmission honeybee viruses is largely obscure due to a lack large-scale longitudinal genomic surveillance on global scale.We report meta-transcriptomic sequencing nearly 2000 samples two most important widely maintained species, as well an associated...

Recently, endorhizospheric microbiota is realized to be able promote the secondary metabolism in medicinal plants, but detailed metabolic regulation metabolisms and whether promotion influenced by environmental factors are unclear yet. Here, major flavonoids endophytic bacterial communities various Glycyrrhiza uralensis Fisch. roots collected from seven distinct places northwest China, as well edaphic conditions, were characterized analyzed. It was found that soil moisture temperature might...

Fine mapping and discovery of candidate genes underlying seed size are important for modern watermelon breeding. Here, by using a high-resolution genetic map whole-genome variation detection aided genome survey sequencing, we fine mapped discovered in watermelon. QTL (quantitative trait locus) identified two pleiotropic QTLs size, namely, qSS4 qSS6, high-density constructed specific length amplified fragment sequencing. qSS6 explained 93.00%, 94.11% 95.26% the phenotypic thousand-seed...

To investigate the feasibility and effectiveness of novel bone hook combined with finger-guided technique in treatment irreducible intertrochanteric femoral fractures elderly. Between January 2021 August 2023, 23 elderly patients were treated technique. There 10 males 13 females; age ranged from 68 to 93 years (mean, 76.2 years). The time injury operation 36 76 hours 51.2 hours). According classification standard proposed by TONG Dake et alin 2021, there cases typeⅠA, 1 case typeⅠB, 6 type...

Abstract Watermelon fruit flesh displays various colors. Although genetic loci underlying these variations are identified, the molecular mechanism remains elusive. Here, we assembled a chromosome-scale reference genome of an elite watermelon and developed integrated maps using single nucleotide polymorphism (SNP) structural variation markers. Several key varients for shape color were identified. Two variants associated with further studied, including one copy number variant (CNV, triplicate...

Breeding habits of essential dependence on flowers for larval food resources have evolved repeatedly in separate lineages the Drosophilidae. However, Impatiens L. never been recognized as hosts drosophilid flies until recently: two Hirtodrosophila species, H. actinia (Okada) and yapingi Gao, were found feeding breeding flowers. During our recent field surveys central southern China, a great number morphologically resembling species collected, almost exclusively from (family Balsaminaceae)...

Egusi watermelon has a unique egusi seed type, which could be useful for breeding both edible seeds and flesh in watermelon. However, the genetic basis of type is not clear. In present study, we first reported that at least two genes with inhibitory epistasis were responsible thin coat (unique type) Inheritance analysis five populations, including F2, BC, BCF2, suggested trait was controlled by suppressor gene together locus (eg) Based on high-throughput sequencing technology, quantitative...

Abstract Seed size is an important agronomic trait in watermelon, but the candidate genes and underlying molecular mechanism are not fully known. In present study, genetic analysis of BC 1 population derived from two parental lines revealed that watermelon seed controlled by a single locus medium seeds dominant to tomato seeds. addition, we constructed linkage map. The total length map was 1629.92 cM, with average distance .16 cM. QTL mapping resequencing identified 13.96‐kb chromosomal...

Tissue phenotypic plasticity facilitates rapid adaptation of organisms to biotic and/or abiotic pressure. The reproductive capacity honey bee workers (Apis mellifera) is plastic and responsive pheromones produced by broods the queen. Egg laying (ELWs), which could reactivate their ovaries lay haploid eggs upon queen lost, have been commonly discussed from many aspects. However, it remains unclear whether midgut homeostasis in ELWs affected during changes. Here, we found that expression...

Gene flow is crucial for maintaining population genetic diversity and increasing the adaptive potential of species. Therefore, identifying factors affecting gene important conservation planning, especially threatened mammals in complex mountain system. In this study, we conducted a microgeographic-scale landscape analysis endangered ecologically isolated Alashan red deer (Cervus elaphus alxaicus) first time. Using data from 329 individuals 11 microsatellite loci, assessed how environmental...

Insect pollinators provide major pollination services for wild plants and crops. Honeybee viruses can cause serious damage to honeybee colonies. However, of other pollinating insects have yet be fully explored. In the present study, we used RNA sequencing investigate viral diversity 50 species insects. A total 3 pathogenic viruses, 8 previously reported 26 novel were identified in sequenced samples. Among these, 7 shown closely related 4 determined potential pathogenicity their hosts. The...

Varroa destructor is a major pathogenic driver of the Western honeybee colony losses globally. Understanding developmental regulation V. critical to develop effective control measures. Development complex biological process regulated by numerous genes and long non-coding RNAs (lncRNAs); however, underlying lncRNAs in development remains unknown. In this study, we analyzed RNA sequencing (RNA-Seq) data derived from four stages female reproductive phase (i.e., egg, protonymph, deutonymph,...

In this article, we report the pathogenicity of a new strain fungus, Rhizopus oryzae to honeybee larvae, isolated from chalkbrood-diseased mummies larvae and pupae collected apiaries in China. Based on morphological observation internal transcribed spacer (ITS) region analyses, pathogenic fungus was identified as R. oryzae. Koch’s postulates were performed determine cause-and-effect isolate fungus. The vitro virulent honeybees tested by artificially inoculating worker lab. for both...

As an enduring Chinese freshwater aquaculture product, the Eriocheir sinensis has a high economic value and is characterized by catadromous life style that undergoes seawater–freshwater migration. However, little known about their gut microbial status as they move from saltwater to acclimatization. Here, we sampled cultivated megalopa three desalination ponds investigated microbiota diversity, community structures biotic interactions stage first juvenile after for 9 days. Our results...

Ascosphaera apis is the causative agent of honey bee chalkbrood disease, and spores are only known source infections. Interference with sporulation therefore a promising way to manage A. apis. The versicolorin reductase gene (StcU-2) ketoreductase protein related melanin biosynthesis. To study StcU-2 in ascospore production apis, CRISPR/Cas9 was used, eight hygromycin B antibiotic-resistant transformants incorporating enhanced green fluorescent (EGFP) were made analyzed. PCR amplification,...

Over the last ten years, bee keeping industry has been struggling to understand and stop sudden widespread loss or collapse of honey colonies, known collectively as Colony Collapse Disorder (CCD), in U.S. around world. While colonies experience many stressors that could cause a colony collapse, we are focusing on quality, health reproductive ability queens. The purpose this line research is identify relationships between pheromone signatures queens quality ultimate goal find reliable,...

This study was conducted to clarify the food compositions of Corbicula bivalves (C. japonica and leana) in tidal rivers with varied landscapes. In order grasp their trophic structures, we measured carbon nitrogen stable isotope ratios collected samples such as growth stages suspended particulate organic matters (SPOMs) landscapes sources, then analyzed relationships IsoSource mixing model software. The ratio measurements clarified that there a large difference ranges C. leana spite same...

This study determined the pathogenicity of a new strain virulent fungus, Rhizopus oryzae , isolated from dead honeybee broods collected apiaries in China, by evaluating its against Apis mellifera worker larvae laboratory. Isolation and characterization fungus were performed on potato dextrose agar media. Species specificity confirmed using internal transcribed spacers (ITS) genes. The cause-and-effect this fungal isolate was verified with Koch’s postulates; vitro pathogen to honeybees tested...