A5078623638- Connective tissue disorders research
- Elasticity and Material Modeling
- Cardiovascular Health and Disease Prevention
- Aortic aneurysm repair treatments
- Williams Syndrome Research
- Protease and Inhibitor Mechanisms
- Aortic Disease and Treatment Approaches
- Cerebrovascular and Carotid Artery Diseases
- Coronary Interventions and Diagnostics
- Peripheral Artery Disease Management
- Cellular Mechanics and Interactions
- Cardiomyopathy and Myosin Studies
- Cell Adhesion Molecules Research
- Cardiovascular Function and Risk Factors
- Intracranial Aneurysms: Treatment and Complications
- Congenital heart defects research
Washington University in St. Louis
2017-2023
Elastin is an important ECM (extracellular matrix) protein in large and small arteries. Vascular smooth muscle cells (SMCs) produce the layered elastic laminae found arteries but synthesize little elastin muscular However, have a well-defined internal lamina (IEL) that separates endothelial (ECs) from SMCs. The extent to which ECs contribute IEL unknown.To use targeted (Eln) deletion mice explore relative contributions of SMCs formation different arteries.We used SMC- EC-specific Cre...
Elastic fibers are present in low quantities tendon, where they located both within fascicles near tenocytes and more broadly the interfascicular matrix (IFM). While elastic have long been known to be significant mechanics of elastin-rich tissue (i.e., vasculature, skin, lungs), recent studies suggested a mechanical role for tendons that is dependent on specific tendon function. However, exact contribution elastin properties different types (e.g., positional, energy-storing) remains unknown....
Elastin is a primary structural protein in the arterial wall that contributes to vascular mechanical properties and degrades with aging. Aging associated stiffening an increase blood pressure. There evidence aging follows different timelines sex. Our objective was investigate how elastin content affects remodeling male female mice We used wild-type (Eln+/+) heterozygous (Eln+/-) at 6, 12, 24 mo of age measured their pressure morphology, structure, content, circumferential stress, stretch...
Elastic fibers provide reversible elasticity to the large arteries and are assembled during development when hemodynamic forces increasing. Mutations in elastic fiber genes associated with cardiovascular disease. Mice lacking expression of elastin ( Eln −/− ), fibulin-4 Efemp2 or lysyl oxidase Lox ) die at birth severe malformations. All three genetic knockout models have defects, aortic wall thickening, arterial tortuosity. However, mice develop stenoses, while ascending aneurysms. We...
Increased arterial stiffness is associated with atherosclerosis in humans, but there have been limited animal studies investigating the relationship between these factors. We bred elastin wildtype (Eln+/+) and heterozygous (Eln+/-) mice to apolipoprotein E (Apoe+/+) knockout (Apoe-/-) fed them normal diet (ND) or Western (WD) for 12 weeks. Eln+/- increased stiffness. Apoe-/- develop on ND that accelerated by WD. It has reported may play a role atherosclerotic plaque progression. found...
Transmural advective transport of solute and fluid was investigated in mouse carotid arteries with either a genetic knockout fibulin-5 (Fbln5-/-) or treatment elastase to determine the influence disrupted elastic fiber matrix on wall properties. Fibulin-5 is an important director assembly. Arteries from Fbln5-/- mice have loose, noncontinuous network were hypothesized reduced resistance transport. Experiments carried out ex vivo at physiological pressure axial stretch. Hydraulic conductance...
Arterial stiffening occurs during natural aging, is associated with an increased risk of adverse cardiovascular events, and can follow different timelines in males females. One mechanism arterial includes remodeling the extracellular matrix (ECM), which alters wall material properties. We used elastin haploinsufficient (Eln+/-) wildtype (Eln+/+) mice to investigate how properties two arteries (ascending aorta carotid artery) change age, sex, ECM composition. a constitutive model by Dong Sun...