A5078771244- Nitric Oxide and Endothelin Effects
- Electron Spin Resonance Studies
- Molecular Sensors and Ion Detection
- Cancer, Hypoxia, and Metabolism
- Sulfur Compounds in Biology
- Redox biology and oxidative stress
- Electrochemical sensors and biosensors
- Mitochondrial Function and Pathology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Free Radicals and Antioxidants
- Cancer, Lipids, and Metabolism
- Metabolism, Diabetes, and Cancer
- Eicosanoids and Hypertension Pharmacology
- ATP Synthase and ATPases Research
- Analytical Chemistry and Sensors
- Electrochemical Analysis and Applications
- Photochemistry and Electron Transfer Studies
- bioluminescence and chemiluminescence research
- Magnolia and Illicium research
- Inflammatory mediators and NSAID effects
- Parkinson's Disease Mechanisms and Treatments
- Radical Photochemical Reactions
- Coenzyme Q10 studies and effects
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
Medical College of Wisconsin
2016-2025
BioEnergetics (United States)
2021
University of Wisconsin–Madison
2017
Universitat de València
2017
Lodz University of Technology
2003-2014
University of Łódź
2001-2006
Cancer cells are long known to exhibit increased aerobic glycolysis, but glycolytic inhibition has not offered a viable chemotherapeutic strategy in part because of the systemic toxicity antiglycolytic agents. However, recent studies suggest that combined glycolysis and mitochondrial function may help overcome this issue. In study, we investigated efficacies mitochondria-targeted drugs (MTD) combination with 2-deoxy-d-glucose (2-DG), compound inhibits glycolysis. Using MTDs, termed Mito-CP...
Boronates, a group of organic compounds, are emerging as one the most effective probes for detecting and quantifying peroxynitrite, hypochlorous acid, hydrogen peroxide. Boronates react with peroxynitrite nearly million times faster than Boronate-containing fluorogenic compounds have been used to monitor real time generation in cells imaging peroxide living animals. This perspective highlights potential applications boronates other fluorescent high-throughput analyses hydroperoxides...
Lung cancer often has a poor prognosis, with brain metastases major reason for mortality. We modified lonidamine (LND), an antiglycolytic drug limited efficacy, to mitochondria-targeted mito-lonidamine (Mito-LND) which is 100-fold more potent. Mito-LND, tumor-selective inhibitor of oxidative phosphorylation, inhibits mitochondrial bioenergetics in lung cells and mitigates cell viability, growth, progression, metastasis xenografts mice. Mito-LND blocks tumor development by inhibiting...
Abstract Metformin (Met) is an approved antidiabetic drug currently being explored for repurposing in cancer treatment based on recent evidence of its apparent chemopreventive properties. Met weakly cationic and targets the mitochondria to induce cytotoxic effects tumor cells, albeit not very effectively. We hypothesized that increasing mitochondria-targeting potential by attaching a positively charged lipophilic substituent would enhance antitumor activity Met. In pursuit this question, we...
Herein we describe a high-throughput fluorescence and HPLC-based methodology for global profiling of reactive oxygen nitrogen species (ROS/RNS) in biological systems. The combined use HPLC detection is key to successful implementation validation this methodology. Included here are methods specifically detect quantitate the products formed from interaction between ROS/RNS fluorogenic probes, as follows: superoxide using hydroethidine, peroxynitrite boronate-based nitric oxide-derived...
Planar bone scintigraphy (PBS) and single-photon emission computed tomography (SPECT) were compared in 19 adults with radiographic evidence of spondylolysis and/or spondylolisthesis. SPECT was more sensitive than PBS when used to identify symptomatic patients sites "painful" defects the pars interarticularis. In addition, allowed accurate localization PBS. 6 patients, or spondylolisthesis unrelated low back pain, images posterior neural arch normal. The authors conclude that is cause are...
Mitoquinone (MitoQ) is a synthetically modified, redox-active ubiquinone compound that accumulates predominantly in mitochondria. We found MitoQ 30-fold more cytotoxic to breast cancer cells than healthy mammary cells. treatment led irreversible inhibition of clonogenic growth through combination autophagy and apoptotic cell death mechanisms. Relatively limited cytotoxicity was seen with the parent coenzyme Q(10.) Inhibition by associated G(1)/S cycle arrest phosphorylation checkpoint...
Abstract Background Recent research has revealed that targeting mitochondrial bioenergetic metabolism is a promising chemotherapeutic strategy. Key to successful implementation of this strategy the use new and improved mitochondria-targeted cationic agents selectively inhibit energy in breast cancer cells, while exerting little or no long-term cytotoxic effect normal cells. Methods In study, we investigated cytotoxicity alterations induced by vitamin E analog (Mito-chromanol, Mito-ChM) its...
Aims: Parkinson's disease (PD) is a neurodegenerative disorder characterized by progressive motor deficits and degeneration of dopaminergic neurons. Caused number genetic environmental factors, mitochondrial dysfunction oxidative stress play role in neurodegeneration PD. By selectively knocking out transcription factor A (TFAM) neurons, the transgenic MitoPark mice recapitulate many signature features disease, including deficits, neuronal loss, protein inclusions. In present study, we...