A5072014297- NMR spectroscopy and applications
- Advanced Electron Microscopy Techniques and Applications
- Molecular spectroscopy and chirality
- Electron and X-Ray Spectroscopy Techniques
- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Various Chemistry Research Topics
- Advanced MRI Techniques and Applications
- Nanopore and Nanochannel Transport Studies
- Advanced Fluorescence Microscopy Techniques
- Force Microscopy Techniques and Applications
- Molecular Junctions and Nanostructures
- Micro and Nano Robotics
- Machine Learning in Materials Science
- Gas Sensing Nanomaterials and Sensors
- Pickering emulsions and particle stabilization
- High Entropy Alloys Studies
- High-Temperature Coating Behaviors
- Polymer Surface Interaction Studies
- Fluid Dynamics and Thin Films
- Photoreceptor and optogenetics research
- Advanced Polymer Synthesis and Characterization
- Fullerene Chemistry and Applications
- Sleep and Work-Related Fatigue
- Photochromic and Fluorescence Chemistry
Peking University
2021-2025
Beijing National Laboratory for Molecular Sciences
2022-2025
Imaging Center
2015-2025
Chang'an University
2024
Hudson Institute
2016-2023
John Wiley & Sons (United States)
2016-2023
Liechtenstein Institute
2023
Linde (United States)
2023
Taishan University
2022
Northeastern University
2022
Reactions give solvents a kick During chemical reaction, the reorganization of solvent molecules not directly in contact with reactants and products is normally viewed as simple diffusion response. Wang et al. studied molecular six common reactions—including copper-catalyzed click reaction Diels-Alder reaction—with pulsed-field gradient nuclear magnetic resonance. They observed boost mobility relative to Brownian that was stronger for catalyzed reactions were studied. The mobilities verified...
Assessing the ergodicity of graphene liquid cell electron microscope measurements, we report that loop states circular DNA interconvert reversibly and numbers follow Boltzmann distribution expected for this molecule in bulk solution, provided dose is low (80-keV energy rate 1-20 e
The reversible modification of proteins with lipoic acid (LPA)-derived polydisulfides (PDS) is an important approach toward the transient regulation and on-demand recovery protein functions. in situ growth PDS from cysteine (Cys) residue a protein, however, has been challenging due to near-equilibrium thermodynamics ring-opening polymerization LPA. Here, we report protein-mediated, aggregation-induced (AIP) amphiphilic LPA-derived monomers at room temperature, which can be performed...
It remains challenging to understand the structural evolution of conjugated polymers from single chains solvated aggregates and film microstructures, although it underpins performance optoelectrical devices fabricated via mainstream solution processing method. With several ensemble visual measurements, here we unravel morphological process a model system isoindigo-based molecules, including hidden molecular assembly pathways, mesoscale network formation, their unorthodox chain dependence....
Using nanobubbles as geometrical confinements, we create a thin water film (∼10 nm) in graphene liquid cell and investigate the evolution of its instability at nanoscale under transmission electron microscopy. The breakdown films, resulting subsequent formation growth nanodroplets, is visualized generalized into different modes. We identified distinct droplet modes by analyzing dynamic processes involving 61 droplets 110 bridges within 31 Graphene Liquid Cells (GLCs). Droplet influenced...
Use of electron-based microscopy in aqueous media has been held back because samples tend to suffer from water radiolysis and other chemical degradation caused by the high energy incident electrons. Here we show that liquid pockets graphene cells at room temperature display significantly improved stability when using deuterated water, D2O. Reporting transmission electron (TEM) experiments based on common imaging conditions, conclude use D2O outperforms adding radical scavengers H2O...
We evaluate critically the use of pulsed gradient spin–echo nuclear magnetic resonance to measure molecular mobility during chemical reactions. With raw NMR spectra available in a public depository, we confirm boosted click reaction (Wang et al. Science 2020 369, 537−541) regardless order field (linearly increasing, linearly decreasing, random sequence). also for Diels–Alder reaction. The conceptual advantage former system is that constant rate implies catalyst concentration, whereas latter...
In quest of the holy grail to "see" how individual molecules interact in liquid environments, single-molecule imaging methods now include liquid-phase electron microscopy, whose resolution can be nanometers space and several frames per second time using an ordinary microscope that is routinely available many researchers. However, with current state art, protocols sound similar those described literature lead outcomes differ. The key challenge achieve sample contrast under a safe dose within...
CO2 is a waste gas, but also crucial C1 resource. The chemical conversion of significant for reducing its concentration in the air and mitigating negative impacts...
The intellectual community focused on nanomotors has recently become interested in extending these concepts to individual molecules. Here, we study a chemical reaction according whose mechanism some intermediate species should speed up while others slow down predictable ways, if the nanomotor hypothesis of boosted diffusion holds. Accordingly, scrutinize absolute coefficient (D) during steps catalytic cycle for CuAAC (copper-catalyzed azide-alkyne cycloaddition click reaction), using proton...
Nanoconfined water plays an important role in broad fields of science and engineering. Classical molecular dynamics (MD) simulations have been widely used to investigate phases under nanoconfinement. The key ingredient MD is the force field. In this study, we systematically investigated performance a recently introduced family globally optimal models, OPC OPC3, TIP4P/2005 describing nanoconfined two-dimensional (2D) ice. Our studies show that melting points monolayer square ice (MSI) all...
Liquid-phase electron microscopy (LP-EM) imaging has revolutionized our understanding of nanosynthesis and assembly. However, the current closed geometry limits its application for open systems. The ubiquitous physical process coffee-ring phenomenon that underpins materials engineering science remains elusive at nanoscale due to lack experimental tools. We introduce a quartz nanopipette liquid cell with tunable dimension requires only standard microscopes. Depending on condition, allows...
Protein/peptide amyloid fibril formation is associated with various neurodegenerative diseases and, hence, has been the subject of extensive studies. From a structure-evolution point view, we now know great deal about initial and final states this process; however, very little its intermediate states. Herein, employ liquid-phase transmission electron microscopy to directly visualize one intermediates formed during aggregation process an amyloid-forming peptide. As shown in figure, find that...
Deep learning methods, U-Net, UNet++, were applied to the analysis of single molecule movies obtained from liquid-phase electron microscopy.
A detailed quantitative nanoscopic description of soft surfaces under dynamic flow is lacking, despite its importance. To better understand the role surface texture in mass transport complex media, we used Förster resonance energy transfer combination with total internal reflectance fluorescence microscopy (FRET-TIRFM) to directly measure laminar slip penetration depth (slip length) on poly(N-isopropylacrylamide) (pNIPAM) thin films (50–110 nm) different grafting densities (0.60, 0.38, and...