A5014613813- Hepatitis C virus research
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Viral-associated cancers and disorders
- Histiocytic Disorders and Treatments
- interferon and immune responses
- MicroRNA in disease regulation
- HIV Research and Treatment
- Immune Cell Function and Interaction
- Polyomavirus and related diseases
- Herpesvirus Infections and Treatments
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Respiratory viral infections research
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Extracellular vesicles in disease
- Galectins and Cancer Biology
- RNA modifications and cancer
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Synthesis and Biological Evaluation
- RNA regulation and disease
University of North Carolina at Chapel Hill
2017-2019
UNC Lineberger Comprehensive Cancer Center
2017
Icahn School of Medicine at Mount Sinai
2010-2016
Albert Einstein College of Medicine
2011
ABSTRACT The liver-specific microRNA miR-122 is required for efficient hepatitis C virus (HCV) RNA replication both in cell culture and vivo . In addition, nonhepatic cells have been rendered more at supporting this stage of the HCV life cycle by expression. This study investigated how influences miR-122-deficient HepG2 line. Expression permitted infectious virion production. When a missing receptor also expressed, these efficiently support viral entry thus entire cycle.
The E2 glycoprotein of hepatitis C virus (HCV) mediates viral attachment and entry into target hepatocytes elicits neutralizing antibodies in infected patients. To characterize the structural functional basis HCV neutralization, we generated a novel panel 78 monoclonal (MAbs) against proteins from genotype 1a 2a strains. Using high-throughput focus-forming reduction or luciferase-based neutralization assays with chimeric infectious containing both genotypes, defined eight MAbs that...
Hepatitis C virus (HCV) is a major cause of liver disease worldwide. A better understanding its life cycle, including the process host cell entry, important for development HCV therapies and model systems. Based on requirement numerous factors, two tight junction proteins claudin-1 (CLDN1) occludin (OCLN), entry has been proposed to be multi-step process. The lack OCLN-specific inhibitors prevented comprehensive analysis this To study role OCLN in we created mutants whose activities could...
Hepatitis C virus (HCV) cell entry is a complex, multistep process requiring numerous host factors, including the tight junction protein claudin‐1 (CLDN1). It not known whether CLDN1 and HCV glycoproteins physically interact. Therefore, focus of this work was to study genetic interactions between HCV. We used CRISPR technology generate knockout (KO) Huh‐7.5 cells, which could be infected by genotype 2a Jc1 unless expression restored. Passage Jc1‐transfected KO cells resulted in selection...
ABSTRACT Hepatitis C virus (HCV) is a leading cause of liver disease worldwide. As HCV infects only human and chimpanzee cells, antiviral therapy vaccine development have been hampered by the lack convenient small-animal model. In this study we further investigate how species tropism modulated at level cell entry. It has previously determined that tight junction protein occludin (OCLN) essential for host entry OCLN more efficient than mouse ortholog mediating To relationship between sequence...
Kaposi's sarcoma-associated herpesvirus (KSHV) is the etiologic agent of three human malignancies, endothelial cell cancer sarcoma, and two B cancers, Primary Effusion Lymphoma multicentric Castleman's disease. KSHV has latent lytic phases viral life cycle, while both contribute to pathogenesis, proteins KSHV-mediated oncogenesis. Reactivation from latency driven by gene transactivator RTA, RTA transcription controlled epigenetic modifications. To identify host chromatin-modifying that are...
Kaposi's sarcoma-associated herpesvirus (KSHV) is a that linked to sarcoma (KS), primary effusion lymphoma (PEL) and multicentric Castleman's disease (MCD). KSHV establishes persistent latent infection in the human host. undergoes periods of spontaneous reactivation where it can enter lytic replication phase its lifecycle. During reactivation, host innate immune responses are activated restrict viral replication. Here, we report NLRX1, negative regulator type I interferon response, important...
Hepatitis C virus (HCV) is the leading cause of liver cancer in Western Hemisphere. HCV infection requires miR-122, which expressed only cells, and thus one reason that replication this occurs efficiently cells hepatic origin. To understand how genetics impact miR-122 usage, we knocked out using clustered regularly interspaced short palindromic repeat (CRISPR) technology adapted to replicate presence noncognate RNAs. In doing so, identified viral mutations allow complete absence miR-122....
Herpesviral deubiquitinating enzymes (DUBs) were discovered in 2005, are highly conserved across the family, and proving to be increasingly important players herpesviral infection. EBV's DUB, BPLF1, is known regulate both cellular viral target activities, yet remains largely unstudied. Our work has implicated BPLF1 a wide range of processes including infectivity, DNA replication, repair. Additionally, knockout delays reduces human B-cell immortalization lymphoma formation humanized mice....
1Department of MicrobiologyIcahn School Medicine at Mount SinaiNew YorkNYUSA *Corresponding author: [email protected]