A5100774923- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Connexins and lens biology
- RNA modifications and cancer
- T-cell and Retrovirus Studies
- CRISPR and Genetic Engineering
- Biochemical and Molecular Research
- Genetic Neurodegenerative Diseases
- Periodontal Regeneration and Treatments
- Reproductive Biology and Fertility
- Transgenic Plants and Applications
- Endoplasmic Reticulum Stress and Disease
- Insect behavior and control techniques
- Glycosylation and Glycoproteins Research
- Insect symbiosis and bacterial influences
- Signaling Pathways in Disease
- Barrier Structure and Function Studies
- S100 Proteins and Annexins
- Fungal and yeast genetics research
- Connective Tissue Growth Factor Research
- Renal and related cancers
- Histone Deacetylase Inhibitors Research
- Acute Kidney Injury Research
Chinese Academy of Sciences
2022-2024
Institute of Zoology
2022-2024
University of Chinese Academy of Sciences
2022-2024
First Affiliated Hospital Zhejiang University
2023
Peking University
2017-2018
Kunming Institute of Zoology
2016
Kunming University of Science and Technology
2016
University College London
2014
Zhejiang University
2010
Intestinal ischemia-reperfusion is associated with the generation of reactive oxygen metabolites as well remote, oxidant-mediated lung injury. Oxidants elicit endothelial redox imbalance and loss vascular integrity by disorganizing several junctional proteins that contribute to maintenance regulation barrier. To determine specific effect on pulmonary barrier integrity, microvascular permeability was determined in lungs animals subjected chemically induced imbalance. The cultured cells also...
Sunflower Trypsin Inhibitor (SFTI-1) analogues have been prepared from simple linear precursors produced either by chemical synthesis or following purification Escherichia coli. We shown, for the first time that these SFTI-1 derived peptide sequences can be converted to circular peptides via selective consecutive acyl transfer reactions, and products synthetic bacterial origin are identical. Preliminary analysis of semi-synthetic confirmed SFTI-I10H as an inhibitor Kallikrein-5 (KLK5)...
Animal density-dependent experiences have profound effects on reproductive strategies with marked fecundity differences. Migratory locust adopts distinct population to cope their respective life cycles, but the mechanisms remain poorly understood. Here, we report that Piwi-interacting RNAs (piRNAs) in germline play key roles this process. We find Piwi protein Liwi1 and piRNAs are highly expressed early developing egg chambers solitarious locusts, which higher than gregarious locusts....
Abstract Parental experiences can affect the phenotypic plasticity of offspring. In locusts, population density that adults experience regulates number and hatching synchrony their eggs, contributing to locust outbreaks. However, pathway signal transmission from parents offspring remains unclear. Here, we find transcription factor Forkhead box protein N1 (FOXN1) responds high activates polypyrimidine tract-binding 1 ( Ptbp1 ) in locusts. FOXN1–PTBP1 serves as an upstream regulator miR-276, a...
Expanded non-coding RNA repeats of CUG and CCUG are the underlying genetic causes for myotonic dystrophy type 1 (DM1) 2 (DM2), respectively. A gain-of-function these pathogenic repeat expansions is mediated at least in part by their abnormal interactions with RNA-binding proteins such as MBNL1 resultant loss activity proteins. To study mechanisms CCUG-repeat an animal model, we created a fly model DM2 that expresses pure, uninterrupted ranging from 16 to 720 length. We show this...
The majority of long noncoding RNAs (lncRNAs) contain transposable elements (TEs). PAHAL, a nuclear-retained lncRNA that is inserted by Gypsy retrotransposon, has been shown to be vital regulator phenylalanine hydroxylase (PAH) gene expression controls dopamine biosynthesis and behavioural aggregation in the migratory locust. However, role retrotransposon transcriptional regulation PAHAL remains unknown. Here, we identified (named element) as an inverted terminal repeat located 3' end...
To investigate the clinical features and genetic defects in four generations of a Chinese family affected with atypical granular corneal dystrophy type I (GCD I).Family history data were recorded. Genomic DNA samples obtained from peripheral blood leukocytes all participated. Exons transforming growth factor-β-induced (TGFBI) gene directly sequenced after being amplified by polymerase chain reaction (PCR), multi-point linkage analysis using microsatellite makers flanking was applied to...
SUMMARY The proteasome is a sophisticated ATP-dependent molecular machine responsible for protein degradation in all eukaryotic cells. It remains elusive how conformational changes of the AAA-ATPase unfoldase regulatory particle (RP) control gating substrate-translocation channel to proteolytic chamber core (CP). Here we report three alternative states ATP-γS-bound human proteasome, which CP gate asymmetrically open, visualized by cryo-EM at near-atomic resolutions. Only four nucleotides are...
The proteasome is a sophisticated ATP-dependent molecular machine responsible for protein degradation in all eukaryotic cells. It remains elusive how conformational changes of the AAA-ATPase unfoldase regulatory particle (RP) control gating substrate-translocation channel to proteolytic chamber core (CP). Here we report three alternative states ATP-γS-bound human proteasome, which CP gate asymmetrically open, visualized by cryo-EM at near-atomic resolutions. Only four nucleotides are stably...
Abstract The proteasome is an ATP-dependent 2.5-megadalton machine responsible for ubiquitylated protein degradation in all eukaryotic cells. Here we present cryo-EM structures of the substrate-engaged human 26S seven conformational states at 2.8-3.6 Å resolution, captured during polyubiquitylated degradation. These visualize a continuum dynamic substrate-proteasome interactions from ubiquitin recognition to processive substrate translocation, which ATP hydrolysis sequentially navigate...