A5071857438- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Ubiquitin and proteasome pathways
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Peptidase Inhibition and Analysis
- Lipid metabolism and biosynthesis
- Plant biochemistry and biosynthesis
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Phytochemicals and Antioxidant Activities
- Plant Genetic and Mutation Studies
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Glycosylation and Glycoproteins Research
- Redox biology and oxidative stress
- Transgenic Plants and Applications
- Plant responses to elevated CO2
South China Normal University
2016-2025
China Tobacco
2024
Beijing Institute of Technology
2022-2024
China Telecom (China)
2023-2024
China Telecom
2023-2024
University of Science and Technology of China
2023-2024
Shandong University of Finance and Economics
2023
Shanghai Institute for Science of Science
2022
Guangxi University
2022
Durham University
2017
Abstract The rice (Oryza sativa) spotted leaf11 (spl11) mutant was identified from an ethyl methanesulfonate–mutagenized indica cultivar IR68 population and previously shown to display a spontaneous cell death phenotype enhanced resistance fungal bacterial pathogens. Here, we have isolated Spl11 via map-based cloning strategy. isolation of the gene facilitated by identification three additional spl11 alleles IR64 collection. predicted SPL11 protein contains both U-box domain armadillo (ARM)...
Ubiquitination plays important roles in plant hormone signal transduction. We show that the RING finger E3 ligase, Arabidopsis thaliana SALT- AND DROUGHT-INDUCED FINGER1 (SDIR1), is involved abscisic acid (ABA)-related stress SDIR1 expressed all tissues of and upregulated by drought salt stress, but not ABA. Plants expressing ProSDIR1-beta-glucuronidase (GUS) reporter construct confirmed strong induction GUS expression stomatal guard cells leaf mesophyll under stress. The green fluorescent...
Gold-nanoparticles-based colorimetric assay is an attractive detection format, but limited by the tedious and ineffective posthybridization manipulations for genomic analysis. Here, we present a new design gene-sensing platform based on clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system. In this strategy, programmable recognition of DNA Cas12a/crRNA RNA Cas13a/crRNA with complementary target activates trans-ssDNA or -ssRNA cleavage. Target-induced ssRNA cleavage...
Previous analysis of transcriptional changes after elicitation Cf-9 transgenic tobacco (Nicotiana tabacum) by Avr9 peptide revealed a rapidly upregulated gene, ACRE276. We show that ACRE276 is transiently induced in wounded leaves within 15 min, but upon elicitor treatment, this upregulation enhanced and maintained until cell death onset tobacco. RNA interference (RNAi) silencing results loss hypersensitive response (HR) specified Cf resistance genes. RNAi plants are also compromised for HR...
Mitochondria and autophagy play important roles in the networks that regulate plant leaf senescence cell death. However, molecular mechanisms underlying interactions between mitochondrial signaling are currently not well understood. This study characterized function of Arabidopsis (Arabidopsis thaliana) AAA-protease gene FtSH4 regulating senescence, finding mediates WRKY-dependent salicylic acid (SA) accumulation signaling. Knockout ftsh4-4 mutant resulted severe death, high levels. The...
BRAHMA (BRM), a SWI/SNF chromatin remodeling ATPase, is essential for the transcriptional reprogramming associated with development and cell differentiation in Arabidopsis thaliana. In this study, we show that loss-of-function mutations BRM led to defective maintenance of root stem niche, decreased meristematic activity, stunted growth. Mutations affected auxin distribution by reducing local expression several PIN-FORMED (PIN) genes cells impaired transcription factor PLETHORA (PLT1) PLT2....
To better understand the mechanism involved in ethylene-induced chilling tolerance harvested banana fruit, a gel-based proteomic study followed by MALDI-TOF-TOF MS was carried out. Banana fruit were treated with 500 ppm ethylene for 12 h and then stored at 6°C. During cold storage, assessed proteins from peel extracted analysis. It observed that pretreatment significantly induced manifesting as increases maximal chlorophyll fluorescence (Fv/Fm) decreased electrolyte leakage. Sixty-four spots...
Heat stress (HS) has serious effects on plant development, resulting in heavy agricultural losses. A critical transcription factor network is involved adaptation to high temperature. DEHYDRATION RESPONSIVE ELEMENT-BINDING PROTEIN2A (DREB2A) a key that functions thermotolerance. The DREB2A protein unstable under normal temperature and degraded by the 26S proteasome; however, mechanism which stability dramatically increases response HS remains poorly understood. In this study, we found of...
Abstract Small peptides modulate multiple processes in plant cells, but their regulation by post-translational modification remains unclear. ROT4 (ROTUNDIFOLIA4) belongs to a family of Arabidopsis non-secreted small peptides, knowledge on its molecular function and how it is regulated limited. Here, we find that S-acylated cells. S-acylation an important form protein lipidation, yet so far has not been reported regulate plants. We show this essential for the plasma membrane association ROT4....
Abstract Abscisic acid (ABA) signaling is crucial for plant responses to various abiotic stresses. The Arabidopsis (Arabidopsis thaliana) transcription factor ABA INSENSITIVE 5 (ABI5) a central regulator of signaling. ABI5 BINDING PROTEIN 1 (AFP1) interacts with and facilitates its 26S-proteasome-mediated degradation, although the detailed mechanism has remained unclear. Here, we report that an ABA-responsive U-box E3 ubiquitin ligase, PLANT U-BOX 35 (PUB35), physically AFP1 ABI5. PUB35...
Abstract Plants maintain stem cells in meristems to sustain lifelong growth; these must have effective DNA damage responses prevent mutations that can propagate large parts of the plant. However, molecular links between cell functions and remain largely unexplored. Here, we report small ubiquitin-related modifier E3 ligase AtMMS21 (for methyl methanesulfonate sensitivity gene21) acts root niche by mediating Arabidopsis (Arabidopsis thaliana). Mutation causes defects during embryogenesis...
Geminiviruses, such as beet severe curly top virus (BSCTV), are a group of DNA viruses that cause plant diseases and agricultural losses. The C4 protein is major symptom determinant in several geminiviruses; however, its regulatory mechanism molecular function cells remain unclear. Here, we show BSCTV S-acylated planta, this post-translational lipid modification necessary for membrane localization functions, especially regulation shoot development host plants. Furthermore, the form interacts...
The annual Zea mays ssp. mexicana L. is a member of teosinte, wild relative the spp. This subspecies has strong growth and regeneration ability, high tiller numbers, protein lysine content as well resistance to many fungal diseases, it can be effectively used in maize improvement. In this study, we reported transcriptome by merging data from untreated control (CK), cold (4℃) drought (PEG2000, 20%) treated plant samples. A total 251,145 transcripts (N50 = 1,269 bp) 184,280 unigenes 923 were...
MMS21 is a SUMO E3 ligase that conserved in eukaryotes, and has previously been shown to be required for DNA repair maintenance of chromosome integrity. Loss the Arabidopsis causes defective meristems dwarf phenotypes.Here, we show role AtMMS21 during gametophyte development. deficient plants are semisterile with shorter mature siliques abortive seeds. The mms21-1 mutant shows reduced pollen number, viability, germination abnormal tube growth. Embryo sac development also compromised mutant....
Abstract Various chloroplast transit peptides (CTP) have been used to successfully target some foreign proteins into chloroplasts, but for other these same CTPs reduced localization efficiencies or fail completely. The underlying cause of the failures remains an open question, and more effective are needed. In this study, we initially observed that two E.coli enzymes, EcTSR EcGCL, failed be targeted rice chloroplasts by commonly-used rbcS peptide (rCTP) were subsequently degraded. Further...