A5000395076- Central Venous Catheters and Hemodialysis
- Vascular Malformations Diagnosis and Treatment
- Glutathione Transferases and Polymorphisms
- Angiogenesis and VEGF in Cancer
- Cardiac Fibrosis and Remodeling
- Genomics, phytochemicals, and oxidative stress
- Protease and Inhibitor Mechanisms
- Vascular Anomalies and Treatments
- Renal and Vascular Pathologies
- Parasites and Host Interactions
- Cell Adhesion Molecules Research
- Vascular Procedures and Complications
- Dialysis and Renal Disease Management
- Renal and related cancers
- Selenium in Biological Systems
- Muscle Physiology and Disorders
- Ion Channels and Receptors
- IL-33, ST2, and ILC Pathways
- Electrospun Nanofibers in Biomedical Applications
- Tuberculosis Research and Epidemiology
- Extracellular vesicles in disease
- Growth Hormone and Insulin-like Growth Factors
- Vascular Malformations and Hemangiomas
- NF-κB Signaling Pathways
- Pharmacogenetics and Drug Metabolism
Baylor College of Medicine
2016-2025
Baylor University
2018
Beijing Anzhen Hospital
2009-2016
Capital Medical University
2009-2016
Army Medical University
2015
Guangzhou First People's Hospital
2015
Guangzhou Medical University
2015
Bloodworks Northwest
2015
University of Washington
2015
Methodist Hospital
2010
Interleukin-6 (IL-6) is an important cytokine participating in multiple biologic activities immune regulation and inflammation. IL-6 has been associated with cardiovascular remodeling. However, the mechanism of hypertensive cardiac fibrosis still unclear. Angiotensin II (Ang II) infusion mice increased expression heart. knockout (IL-6-/-) reduced Ang II-induced fibrosis: 1) Masson trichrome staining showed that significantly fibrotic areas wild-type mouse heart, which was greatly suppressed...
Objective— Inflammatory responses play a pivotal role in the pathogenesis of hypertensive cardiac remodeling. Macrophage recruitment and polarization contribute to development fibrosis. Although serum-glucocorticoid regulated kinase 1 (SGK1) is key mediator fibrosis, its regulating macrophage function leading fibrosis has not been investigated. We aimed determine mechanism by which SGK1 regulates inflammatory process, thus contributing Methods Results— After angiotensin II infusion mice,...
In damaged kidneys, increased extracellular matrix (ECM) and tissue stiffness stimulate kidney fibrosis through incompletely characterized molecular mechanisms. The transcriptional coactivators yes-associated protein (Yap) coactivator with PDZ-binding motif (Taz) function as mechanosensors in cancer cells have been implicated the regulation of myofibroblasts kidney. We hypothesized that development depends on Yap-induced activation proliferation fibroblasts. mice, Yap expression renal...
To explore the role of lipid peroxidation (LPO) products in initial phase stress mediated signaling, we studied effect mild, transient oxidative or heat on parameters that regulate cellular concentration 4-hydroxynonenal (4-HNE). When K562 cells were exposed to mild shock (42 °C, 30 min) (50 µmH<sub>2</sub>O<sub>2</sub>, 20 and allowed recover for 2 h, there was a severalfold induction hGST5.8, which catalyzes formation glutathione-4-HNE conjugate (GS-HNE), RLIP76, mediates transport GS-HNE...
Active transport of conjugated and unconjugated electrophiles out cells is essential for cellular homeostasis. We have previously identified in human tissues a transporter, DNP-SG [S-(2,4-dinitrophenyl)glutathione] ATPase, capable carrying this function [Awasthi et al. (1998) Biochemistry 37, 5231−5238, 5239−5248]. now report the cloning ATPase. The sequence cDNA clone was identical to that RLIP76, known Ral-binding protein. RLIP76 expressed E. coli purified by affinity chromatography....
Objective— Activation and proliferation of vascular smooth muscle cells (VSMCs) occur in the venous neointima vein grafts. VSMCs a grafted are subjected to mechanical stretch; our goal is understand essential stretch-regulated signals that influence during neointimal formation Methods Results— In cultured VSMCs, stretch induces upregulation both IGF-1 IGF-1R. Stretch sustained tyrosine phosphorylation IGF-1R its substrate, IRS-1; these responses were related stretch-induced activation Src...
Interleukin-12 is essential for the differentiation of naïve T cells into interferon-γ-producing cells, which regulate inflammatory responses. We investigated this process regulating hypertension-induced cardiac fibrosis.Mice infused with angiotensin II showed a marked increase in interleukin-12p35 expression macrophages. The degree fibrosis was significantly enhanced knockout (p35-KO) mice compared wild-type (WT) littermates response to II. Fibrotic hearts p35-KO increased accumulation...
Background The inflammatory response plays a critical role in hypertension-induced cardiac remodeling. We aimed to study how interaction among cells causes responses the process of hypertensive fibrosis. Methodology/Principal Findings Infusion angiotensin II (Ang II, 1500 ng/kg/min) mice rapidly induced expression interferon γ (IFN-γ) and leukocytes infiltration into heart. To determine IFN-γ on inflammation remodeling, both wild-type (WT) IFN-γ-knockout (KO) were infused Ang for 7 days,...
Background Cathepsin S (Cat S) is overexpressed in human atherosclerotic and aneurysmal tissues may contributes to degradation of extracellular matrix, especially elastin, inflammatory diseases. We aimed define the role Cat cardiac inflammation fibrosis induced by angiotensin II (Ang II) mice. Methods Results S-knockout S−/−) littermate wild-type (WT) C57BL/6J mice were infused continuously with Ang (750 ng/kg/min) or saline for 7 days. S−/− showed severe fibrosis, including elevated...
Background: Arteriovenous (AV) fistulas are the preferred access for dialysis but have a high incidence of failure. This study aims to understand crosstalk between skeletal muscle catabolism and AV fistula maturation Methods: Skeletal metabolism were evaluated in mice with chronic kidney disease (CKD). The roles myostatin YAP1 regulating transdifferentiation adventitial mesenchymal stem cells (mesenchymal cells) intima hyperplasia investigated. Nanoparticles carrying inhibitor, verteporfin,...
Angiotensin II (Ang II) promotes vascular inflammation and remodeling via activation of nuclear factor kappaB (NF-kappaB)-mediated transcription proinflammatory genes such as interleukin-6 (IL-6). We examined the signaling mechanism whereby Ang activates NF-kappaB in smooth muscle cells (VSMCs).Ang treatment did not increase phosphorylation inhibitor kappaBalpha (IkappaBalpha) or IkappaBbeta decrease their levels. In contrast, mitogen-activated protein kinase kinase-1 (MEK1) inhibition...
Dyslipidemia increases the risks for atherosclerosis in part by impairing endothelial integrity; progenitor cells (EPCs) play a pivotal role reendothelialization. In this study, we investigated mechanism whereby oxidized low-density lipoprotein (oxLDL) affects function of differentiated EPCs (EDCs). EDCs expanded vitro from isolated human cord blood, measured EDC responses to both copper-oxidized LDL and L5, an electronegative minimally vivo patients with hypercholesterolemia. OxLDL induced...
Neointima formation causes the failure of 60% arteriovenous fistulas (AVFs) within 2 years. Neointima-forming mechanisms are controversial but possibly linked to excess proinflammatory responses and dysregulated Notch signaling. To identify how AVFs fail, we anastomosed carotid artery internal jugular vein in normal uremic mice compared these findings with those failed from patients ESRD. Endothelial cells (ECs) or expressed mesenchymal markers (FSP-1 and/or α-SMA) exhibited increased...
Hemodialysis patients depend on arteriovenous fistulas (AVF) for vascular access. Unfortunately, their 2-yr primary patency rate is only 60% because of AVF clog due to intimal hyperplasia at the venous anastomosis. Chronic kidney disease (CKD) can increase neointima formation by unknown mechanisms. A new mouse model was created, and mechanisms CKD in AVFs were investigated. We created mice anastomosing common carotid artery internal jugular vein. induced [BUN (blood urea nitrogen) control...
Objective— Hypoxic pulmonary hypertension (HPH) is characterized by proliferative vascular remodeling. Abnormal artery smooth muscle cells proliferation and endothelial dysfunction are the primary cellular bases of AQP1 (aquaporin-1) regulated oxygen level has been observed to play a role in migration cells. The HPH pathogenesis not directly determined date. To determine possible roles explore its mechanisms. Approach Results— Aqp1 knockout mice were used, model was established this study....
Insulin-like growth factor-1 receptor (IGF-1R) can regulate vascular homeostasis and endothelial function. We studied the role of IGF-1R in oxidative stress-induced dysfunction. Unilateral ureteral obstruction (UUO) was performed wild-type (WT) mice with cell (EC)-specific knockout (KO). After UUO KO mice, barrier dysfunction more severe than WT as seen by increased inflammatory infiltration (VE)-cadherin phosphorylation. interstitial fibroblast accumulation enhanced extracellular protein...