A5088458647- Single-cell and spatial transcriptomics
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Hedgehog Signaling Pathway Studies
- Long-Term Effects of COVID-19
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- T-cell and B-cell Immunology
- Respiratory viral infections research
- Glioma Diagnosis and Treatment
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Chromatin Remodeling and Cancer
- Immune cells in cancer
- Respiratory Support and Mechanisms
- Micro and Nano Robotics
- Healthcare Education and Workforce Issues
- interferon and immune responses
- vaccines and immunoinformatics approaches
- Cancer-related molecular mechanisms research
- Protein Degradation and Inhibitors
- Machine Learning and Data Classification
- Immunotherapy and Immune Responses
- Innovative Human-Technology Interaction
- Education and Learning Interventions
Emory University
2021-2024
RELX Group (United States)
2022
University of North Carolina at Chapel Hill
2018-2022
Meta (United States)
2022
Menlo School
2022
Targeting oncogenic pathways holds promise for brain tumor treatment, but inhibition of Sonic Hedgehog (SHH) signaling has failed in SHH-driven medulloblastoma. Cellular diversity within tumors and reduced lineage commitment can undermine targeted therapy by increasing the probability treatment-resistant populations. Using single-cell RNA-seq tracing, we analyzed cellular medulloblastomas transgenic, medulloblastoma-prone mice, responses to SHH-pathway inhibitor vismodegib. In untreated...
Abstract Troubling disparities in COVID-19–associated mortality emerged early, with nearly 70% of deaths confined to Black/African American (AA) patients some areas. However, targeted studies on this vulnerable population are scarce. Here, we applied multiomics single-cell analyses immune profiles from matching airways and blood samples Black/AA during acute SARS-CoV-2 infection. Transcriptional reprogramming infiltrating IFITM2+/S100A12+ mature neutrophils, likely recruited via the...
ABSTRACT Pre-existing anti-interferon alpha (anti-IFN-α) autoantibodies in blood are associated with susceptibility to life-threatening COVID-19. However, it is unclear whether anti-IFN-α the airways – initial site of infection can also determine disease outcomes. In this study, we developed a new multiparameter technology, flowBEAT, quantify and profile isotypes anti-SARS-CoV-2 antibodies longitudinal samples collected over 20 months from airway matching 129 donors mild, moderate, severe We...
Netrin-1 signaling is an essential prototypical neuronal guidance mechanism during embryonic development that also regulates tumor cell survival in a variety of adult cancer entities. In line with these data, monoclonal netrin-1 blocking antibody (anti-netrin-1 mAb/NP137) has been preclinically developed and blockade recently investigated phase 1 2 clinical trials several cancers. Here, we investigate the role most common malignant pediatric brain cancer, Medulloblastoma. Interestingly, find...
SRY (sex determining region Y)-box 2 (SOX2)-labeled cells play key roles in chemoresistance and tumor relapse; thus, it is critical to elucidate the mechanisms propagating them. Single-cell transcriptomic analyses of most common malignant pediatric brain tumor, medulloblastoma (MB), revealed existence astrocytic
Abstract It is unclear why medulloblastoma patients receiving similar treatments experience different outcomes. Transcriptomic profiling identified subgroups with prognoses, but in each subgroup, individuals remain at risk of incurable recurrence. To investigate similar-appearing tumors produce variable outcomes, we analyzed medulloblastomas triggered transgenic mice by a common driver mutation expressed points brain development. We genetically engineered to express oncogenic SmoM2 ,...
Abstract CD8+ T cells can potentiate long-lived immunity against COVID-19. We screened longitudinally-sampled convalescent human donors SARS-CoV-2 tetramers and identified a participant with an immunodominant response residues 322 to 311 of nucleocapsid (Nuc322–331), peptide conserved in all variants concern reported date. conducted 38-parameter cytometry by time flight on tetramer-identified Nuc322–331–specific CD4+ recognizing the entire spike proteins, took 32 serological measurements....
Single-cell transcriptomics enables the definition of diverse human immune cell types across multiple tissues and disease contexts. Further deeper biological understanding requires comprehensive integration single-cell omics (transcriptomic, proteomic, cell-receptor repertoire). To improve identification accuracy cell-type classification in multi-omics datasets, we developed SuPERR, a novel analysis workflow to increase resolution clustering allow for discovery previously hidden subsets. In...
Preexisting anti–interferon-α (anti–IFN-α) autoantibodies in blood are associated with susceptibility to life-threatening COVID-19. However, it is unclear whether anti–IFN-α the airways, initial site of infection, can also determine disease outcomes. In this study, we developed a multiparameter technology, FlowBEAT, quantify and profile isotypes anti–severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies longitudinal samples collected over 20 months from airways 129 donors...
Biomedical research often produces high-dimensional data confounded by batch effects such as systematic experimental variations, different protocols and subject identifiers. Without proper correction, low-dimensional representation of might encode reproduce the same variations observed in original data, compromise interpretation results. In this article, we propose a novel procedure to remove from embeddings obtained with t-SNE dimensionality reduction. The proposed methods are based on...
Humans are social animals living in societies with most of their activities occurring settings, characterized by multiple actors, the crossing individual behavioral paths, interactions between participants themselves and material (or immaterial) setting components. We theorize that virtual conferences, like physical ones, have a dual-space structure where two types (content transfer intercourse) intertwine. Understanding what happens such complex events requires minutely analyzing this...
Abstract Single-cell RNA sequencing (scRNA-seq) can reveal accurate and sensitive abundance in a single sample, but robust integration of multiple samples remains challenging. Large-scale scRNA-seq data generated by different workflows or laboratories contain batch-specific systemic variation. Such variation challenges confounding sample-specific biology with undesirable effects. Therefore, there is need for guidance selecting computational experimental approaches to minimize impacts on...
Immersive virtual reality (VR) simulations are gaining prominence in undergraduate nursing education because they (a) can mimic the dynamism of clinical settings for students, (b) enable educators to scaffold theory-practice integration and multiple skill development, (c) provide programs with a costeffective curricular solution supporting practice-readiness. In this paper, we investigate first- second-year students' experiences Simulation Learning System Virtual Reality (SLS VR) during...
CD8+ T cells are important antiviral effectors that can potentiate long-lived immunity against COVID-19, but a detailed characterization of these has been hampered by technical challenges. We screened 21 well-characterized, longitudinally-sampled convalescent donors recovered from mild COVID-19 collection SARS-CoV-2 tetramers, and identified one participant with an immunodominant response Nuc322-331, peptide is conserved in all the variants-of-concern reported to date. conducted 38-parameter...
Abstract Patients with medulloblastoma are typically treated a narrow range of therapies, but may experience widely divergent outcomes; 80-90% become long-term survivors while 20% develop incurable recurrence. Transcriptomic profiling has identified four subgroups different recurrence risks, outcomes remain variable for individual patients within each subgroup. To gain new insight into why similar-appearing tumors have outcomes, we examined how the timing tumor initiation effects...
Abstract Single-cell transcriptomics enables the definition of diverse human immune cell types across multiple tissues and disease contexts. Still, deeper biological understanding requires comprehensive integration single-cell omics (transcriptomic, proteomic, cell-receptor repertoire). To improve identification accuracy cell-type classification in multi-omics datasets, we developed SuPERR-seq, a novel analysis workflow to increase resolution clustering allow for discovery previously hidden...
Publicly deploying research chatbots is a nuanced topic involving necessary risk-benefit analyses. While there have recently been frequent discussions on whether it responsible to deploy such models, has far less focus the interaction paradigms and design approaches that resulting interfaces should adopt, in order achieve their goals more effectively. We aim pose, ground, attempt answer HCI questions involved this scope, by reporting mixed-methods user study conducted recent chatbot. find...
Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the ensuing COVID-19 pandemic have caused ∼40 million cases over 648,000 deaths in United States alone. Troubling disparities COVID-19-associated mortality emerged early, with nearly 70% of confined to Black/African-American (AA) patients some areas, yet targeted studies within this demographic are scant. Multi-omics single-cell analyses immune profiles from airways matching blood samples Black/AA revealed low viral...
The use of SMO inhibitors for SHH-subtype medulloblastoma has been both promising and problematic. inhibitor vismodegib met safety efficacy criteria to be FDA-approved the treatment basal cell carcinoma. In medulloblastoma, however, therapy complicated by emergence resistance during treatment. We hypothesized that is driven a subset cells with medulloblastomas remain proliferative despite inhibition eventually drive recurrence. To test this hypothesis, we modeled in transgenic mice...