A5084400126- Electron Spin Resonance Studies
- Catalytic Processes in Materials Science
- Lanthanide and Transition Metal Complexes
- Metal-Organic Frameworks: Synthesis and Applications
- Industrial Gas Emission Control
- Proteins in Food Systems
- Covalent Organic Framework Applications
- Spectroscopy and Quantum Chemical Studies
- Advanced Photocatalysis Techniques
- Protein Structure and Dynamics
- Gas Sensing Nanomaterials and Sensors
- Magnetism in coordination complexes
- Advanced NMR Techniques and Applications
- Lipid Membrane Structure and Behavior
- Electrochemical sensors and biosensors
- Layered Double Hydroxides Synthesis and Applications
- Protein Interaction Studies and Fluorescence Analysis
- Enzyme Catalysis and Immobilization
- Crystal structures of chemical compounds
- Metal complexes synthesis and properties
- DNA and Nucleic Acid Chemistry
- Microencapsulation and Drying Processes
- Catalysis and Oxidation Reactions
- Food composition and properties
- Thermochemical Biomass Conversion Processes
South China University of Technology
2025
North Dakota State University
2016-2024
Jiangnan University
2021-2024
Dakota State University
2018-2024
Hebei University of Science and Technology
2020-2023
State Key Laboratory of Food Science and Technology
2023
The Ohio State University
2019-2021
Wells Fargo (United States)
2018-2021
University of Science and Technology Beijing
2015-2020
Aluminum Corporation of China (China)
2018
How a receptor transmits signal G protein–coupled receptors (GPCRs) transmit diverse external signals into the cell. When activated by an outside stimulus, they bind to protein inside cell and accelerate exchange of bound guanosine diphosphate (GDP) nucleotide for triphosphate, which initiates intercellular signaling. Dror et al. used atomic-level molecular dynamics simulations show how GPCRs enhance GDP release. The is dynamic frequently adopts conformation that exposes even without bound....
Pulsed electron spin resonance (ESR) dipolar spectroscopy (PDS) in combination with site-directed labeling is unique providing nanometer-range distances and distributions biological systems. To date, most of the pulsed ESR techniques require frozen solutions at cryogenic temperatures to reduce rapid relaxation rate prevent averaging electron-electron interaction due molecular tumbling. enable measurements liquid solution, we are exploring a triarylmethyl (TAM)-based label relatively long...
Enzyme immobilization in metal-organic frameworks (MOFs) offers retained enzyme integrity and activity, enhanced stability, reduced leaching. Trapping enzymes on MOF surfaces would allow for catalysis involving large substrates. In both cases, the catalytic efficiency selectivity depend not only integrity/concentration but also orientation. However, it has been a challenge to determine orientation of that are supported solid matrices, which is even more challenging immobilized/trapped MOFs...
Biocatalysis of large-sized substrates finds wide applications. Immobilizing the involved enzymes on solid supports improves biocatalysis yet faces challenges such as enzyme structural perturbation, leaching, and low cost-efficiencies, depending immobilization strategies/matrices. Carbon nanotubes (CNTs) are attractive matrices but challenged by leaching (physical adsorption) or perturbation (covalent linking). Zeolitic imidazolate frameworks (ZIFs) overcome these issues. However, our recent...
Nanofluidic ion transport holds high promise in bio-sensing and energy conversion applications. However, smart nanofluidic devices with flux modulable capabilities remain to be realised. Herein, we demonstrate based on oriented two-dimensional covalent organic framework (2D COF) membranes vertically aligned nanochannel arrays that achieved a 2-3 orders of magnitude higher compared conventional single-channel devices. The surface-charge-governed conductance is dominant for electrolyte...
Type I cadherin cell-adhesion proteins are similar in sequence and structure yet different enough to mediate highly specific cell-cell recognition phenomena. It has previously been shown that small differences the homophilic heterophilic binding affinities of type family members can account for differential cell-sorting behavior. Here we use a combination X-ray crystallography, analytical ultracentrifugation, surface plasmon resonance double electron-electron (DEER) electron paramagnetic...
As a first-line vertebrate immune defense, the polymeric immunoglobulin receptor (pIgR) transports IgA and IgM across epithelia to mucosal secretions, where cleaved ectodomain (secretory component; SC) becomes component of secretory antibodies, or when unliganded, binds excludes bacteria. Here we report 2.6Å crystal structure unliganded human SC (hSC) comparisons with 1.7Å teleost fish (tSC), an early pIgR ancestor. The hSC comprises five immunoglobulin-like domains (D1-D5) arranged as...
Chemical functionalization or docking of transition-metal ions in covalent organic frameworks (COFs) is importance for calibrating properties and widening potential applications. In this work, we demonstrate the successful decoration COF with vanadium as exemplified context post-synthetically modifying two-dimensional that features eclipsed stacking structure, large pores, hydroxyl functionalities, high thermal chemical stability using vanadyl acetylacetonate. The potent catalytic behavior...
Pea protein hydrolysate (PPH) is successfully conjugated with gum arabic (GA) through Maillard-driven chemistry. The effect of cross-linking conjugation on the structure, solubility, volatile substances, emulsification, and antioxidative activity glyco-PPH investigated, found to improve all properties. formation confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Fourier-transform infrared (FTIR), scanning electron microscopy (SEM). Size exclusion...
Although enzyme immobilization has improved many areas, biocatalysis involving large-size substrates is still challenging for platform design because of the protein damage under often "harsh" reaction conditions required these reactions. Our recent efforts indicate potential using Metal–Organic Frameworks (MOFs) to partially confine enzymes on surface MOF-based composites while offering sufficient substrate contact. Still, improvements are expand feasible pH range and efficiency contacting...
Immobilizing enzymes on nanoparticles (NPs) enhances the cost-efficiency of biocatalysis; however, when substrates are large, it becomes difficult to separate enzyme@NP from products while avoiding leaching or damage in reaction medium. Metal–organic framework (MOF)-coated magnetic NPs (MNPs) offer efficient separation and enhanced enzyme protection; involved enzymes/substrates have be smaller than MOF apertures. A potential solution these challenges is coprecipitating metal/ligand with MNP...
We present the measurement of Cu(2+)-Cu(2+) and Cu(2+)-nitroxide distance distributions using double electron-electron resonance (DEER) on a proline-based peptide an alanine-based peptide. The contains two well-characterized Cu(2+) binding segments, PHGGGW, separated by seven proline residues. PHGGGW segment at one end nitroxide spin label attached to cysteine residue close other DEER experiments were performed several external magnetic fields offsets probe orientational effects Cu(2+)-based...
The relationship between DNA sequence recognition and catalytic specificity in a DNA-modifying enzyme was explored using paramagnetic Cu 2+ ions as probes for ESR spectroscopic biochemical studies. Electron spin echo envelope modulation spectroscopy establishes that coordinates to histidine residues the EcoRI endonuclease homodimer bound its specific site. coordinated His were identified by unique use of -ion based long-range distance constraints. Double electron-electron resonance data...