Abstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives constitute rich sources diversity for resistance. Here, we report map-based cloning resistance gene Pm13 from species Aegilops longissima . encodes a mixed lineage kinase domain-like (MLKL) protein that contains an N-terminal-domain MLKL (MLKL_NTD) domain in its N-terminus and C-terminal serine/threonine (STK) domain. function validated by mutagenesis, silencing,...

Abstract Powdery mildew is a devastating disease that affects wheat yield and quality. Wheat wild relatives represent valuable sources of resistance genes. Cloning characterization these genes will facilitate their incorporation into breeding programs. Here, we report the cloning Pm57 , powdery gene from Aegilops searsii . It encodes tandem kinase protein with putative kinase-pseudokinase domains followed by von Willebrand factor A domain (WTK-vWA), being ortholog Lr9 mediates leaf rust...

Genomic prediction is a promising approach for accelerating the genetic gain of complex traits in wheat breeding. However, increasing accuracy (PA) genomic (GP) models remains challenge successful implementation this approach. Multivariate have shown promise when evaluated using diverse panels unrelated accessions; however, limited information available on their performance advanced breeding trials. Here, we used multivariate GP to predict multiple agronomic 314 and elite lines winter 10...

Integrating high-throughput phenotyping (HTP) based traits into phenomic and genomic selection (GS) can accelerate the breeding of high-yielding climate-resilient wheat cultivars. In this study, we explored applicability Unmanned Aerial Vehicles (UAV)-assisted HTP combined with deep learning (DL) for or multi-trait (MT) prediction grain yield (GY), test weight (TW), protein content (GPC) in winter wheat. Significant correlations were observed between agronomic HTP-based across different...

Successful seedling establishment depends on the optimum depth of seed placement especially in drought-prone conditions, providing an opportunity to exploit subsoil water and increase winter survival wheat. Coleoptile length is a key determinant for appropriate at which can be sown. Thus, understanding genetic basis coleoptile necessary important wheat breeding. We conducted genome-wide association study (GWAS) using diverse panel 298 genotypes dissect architecture length. identified nine...

Optimizing wheat height to maximize yield has been an important aspect which is evident from a successful example of green revolution. Dwarfing genes (Rht) are known for gains due lodging resistance and partitioning assimilates into ear. The available commercially exploited sources dwarfism in Indian spring Rht1 Rht2 inspite availability over 20 dwarfing genes. Rht8 Gibberellic acid sensitive gene another reduced some Mediterranean countries. Two F2 populations segregating with each...

Leaf rust caused by Puccinia triticina Eriks is one of the most problematic diseases wheat throughout world. The gene Lr42 confers effective resistance against leaf at both seedling and adult plant stages. Previous studies had reported to be recessive dominant in hexaploid wheat; however, diploid Aegilops tauschii (TA2450), we found studying segregation two independent F2 their F2:3 populations. We further fine-mapped using a KS93U50/Morocco F5 recombinant inbred line (RIL) population 3.7 cM...

Abstract Background In the late 1920s, A. E. Watkins collected about 7000 landrace cultivars (LCs) of bread wheat ( Triticum aestivum L.) from 32 different countries around world. Among which 826 LCs remain viable and could be a valuable source superior/favorable alleles to enhance disease resistance in wheat. present study, core set 121 LCs, captures majority genetic diversity collection, was evaluated for identifying novel sources against tan spot, Stagonospora nodorum blotch (SNB),...

Fusarium head blight (FHB), also known as scab, is a devastating fungal disease of wheat that causes significant losses in grain yield and quality. Quantitative inheritance cumbersome phenotyping make FHB resistance challenging trait for direct selection breeding. Genomic to predict traits has shown promise several studies. Here, we used univariate multivariate genomic prediction models evaluate the accuracy (PA) different using 476 elite advanced breeding lines developed by South Dakota...

Abstract Fusarium head blight (FHB) is a fungal disease posing major threat to wheat production. Plant breeding that leverages genotyping an effective method improve the genetic resistance of cultivars. Started in 1995, uniform regional scab nursery (URSN) consists germplasm from several public programs Northern US region. Its main objective showcase new sources and enable exchange among cooperators; however, data URSN have not been studied. Phenotypic genotypic this were gathered, as well...

Fusarium head blight (FHB) remains one of the most destructive diseases wheat (Triticum aestivum L.), causing considerable losses in yield and end-use quality. Phenotyping FHB resistance traits, Fusarium-damaged kernels (FDK), deoxynivalenol (DON), is either prone to human biases or resource expensive, hindering progress breeding for FHB-resistant cultivars. Though genomic selection (GS) can be an effective way select these inaccurate phenotyping a hurdle exploiting this approach. Here, we...

Abstract Key message Multi-environmental characterization of flag leaf morphology traits in the US winter wheat revealed nine stable genomic regions for different leaf-related including a major region governing angle. Flag is primary contributor to accumulating photosynthetic assimilates. (FLM) determine overall canopy structure and capacity intercept light, thus influencing efficiency. Hence, understanding genetic control these could be useful breeding desirable ideotypes wheat. We used...

A better understanding of the genetic control spike and kernel traits that have higher heritability can help in development high-yielding wheat varieties. Here, we identified marker-trait associations (MTAs) for various spike- kernel-related winter (Triticum aestivum L.) through genome-wide association studies (GWAS). An mapping panel comprising 297 hard accessions from U.S. Great Plains was evaluated eight three different environments. GWAS using 15,590 single-nucleotide polymorphisms...

Improvement of end-use quality remains one the most important goals in hard winter wheat (HWW) breeding. Nevertheless, evaluation traits is confined to later development generations owing resource-intensive phenotyping. Genomic selection (GS) has shown promise facilitating for quality; however, lower prediction accuracy (PA) complex a challenge GS implementation. Multi-trait genomic (MTGP) models can improve PA by incorporating information on correlated secondary traits, but these remain be...

Abstract Stagonospora nodorum blotch (SNB) is an economically important wheat disease caused by the necrotrophic fungus Parastagonospora . SNB resistance in controlled several quantitative trait loci (QTLs). Thus, identifying novel resistance/susceptibility QTLs crucial for continuous improvement of resistance. Here, hard winter association mapping panel (HWWAMP) comprising accessions from breeding programs Great Plains region US, was evaluated and effectors (NEs) sensitivity at seedling...

Fusarium head blight (FHB), caused by the fungus graminearum Schwabe is an important disease of wheat that causes severe yield losses along with serious quality concerns. Incorporating host resistance from either wild relatives, landraces, or exotic materials remains challenging and has shown limited success. Therefore, a better understanding genetic basis native FHB in hard winter (HWW) combining it major quantitative trait loci (QTLs) can facilitate development FHB-resistant cultivars. In...

Abstract Fusarium head blight (FHB) is one of the most destructive fungal diseases affecting wheat ( Triticum aestivum ). Moreover, it notorious for producing mycotoxin deoxynivalenol (DON), posing a significant global threat to food and feed safety. Traditional methods like enzyme‐linked immunosorbent assay (ELISA) gas chromatography‐mass spectrometry (GC‐MS) are commonly used assess DON levels in grain or flour samples time‐consuming expensive. Therefore, faster, cost‐effective method...

<title>Abstract</title> Powdery mildew is a devastating disease that affects wheat yield and quality. Despite wild relatives being valuable source of resistance genes, their incorporation into improvement constrained by adverse effects on agronomic traits, difficulty in isolating genes poor understanding the mechanisms. Here, we report cloning Pm57, first gene isolated from Aegilops searsii. It encodes an unusual tandem kinase (WTK) protein with putative kinase-pseudokinase domains followed...

Abstract End‐use and processing traits in wheat ( Triticum aestivum L.) are crucial for varietal development but often evaluated only the advanced stages of breeding program due to amount grain needed labor‐intensive phenotyping assays. Advances genomic resources have provided new tools address selection these complex earlier process. We used association mapping identify key variants underlying various end‐use quality evaluate usefulness prediction hard red spring from Northern United...

Leaf rust, caused by Puccinia triticina ( Pt ) is among the most devastating diseases posing a significant threat to global wheat production. The continuously evolving virulent races in North America calls for exploring new sources of leaf rust resistance. A diversity panel 365 bread accessions selected from worldwide population landraces and cultivars was evaluated at seedling stage against four (TDBJQ, TBBGS, MNPSD and, TNBJS). wide distribution responses observed. Majority genotypes...

Abstract Wheat powdery mildew is one of the most destructive diseases threatening global wheat production. The wild relatives comprise rich sources diversity for resistance. Here, we report map-based cloning broad-spectrum resistance gene Pm13 from species Aegilops longissima. encodes a novel mixed lineage kinase domain-like (MLKL) protein which contains an N-terminal HeLo domain and C-terminal authentic serine/threonine (STK) domain, unique architecture that has not been reported in plant...

Development and deployment of wheat varieties with high yields, wide adaptability, good quality, multiple-resistance to abiotic biotic stresses, efficient response fertilizers have greatly contributed global sustainable production. The genomic composition key commercial variety can help understand the genetic basis underlying development new permit increased breeding efficiency. In this study, we report chromosomal compositions BN207, presently leading in southern region Huang-Huai River...

Abstract Leaf rust, caused by Puccinia triticina (Pt) is among the most devastating diseases posing a significant threat to global wheat production. The continuously evolving virulent Pt races in North America calls for exploring new sources of leaf rust resistance. A diversity panel 365 bread accessions selected from worldwide population landraces and cultivars was evaluated at seedling stage against four (TDBJQ, TBBGS, MNPSD and, TNBJS). wide distribution responses observed. Majority...