A5031535321- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Spinal Cord Injury Research
- Nerve Injury and Rehabilitation
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- Photoacoustic and Ultrasonic Imaging
- Single-cell and spatial transcriptomics
- Molecular Biology Techniques and Applications
- Nitric Oxide and Endothelin Effects
- Ultrasound and Hyperthermia Applications
- Cervical and Thoracic Myelopathy
- Neuroscience and Neural Engineering
- S100 Proteins and Annexins
- Ultrasound Imaging and Elastography
- Pluripotent Stem Cells Research
- Peripheral Nerve Disorders
- Traumatic Brain Injury and Neurovascular Disturbances
- Supramolecular Self-Assembly in Materials
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Cardiovascular Issues in Pregnancy
- Anesthesia and Neurotoxicity Research
- Cell death mechanisms and regulation
- RNA regulation and disease
University of Calgary
2015-2025
Allen Institute for Brain Science
2019-2022
University of Hong Kong
2006-2019
Chinese University of Hong Kong
2006-2019
HKU-Pasteur Research Pole
2013-2016
Center for Human Reproduction
2012
University Grants Committee
2006
INhibitor of Growth (ING1-5) proteins are epigenetic readers that target histone acetyltransferase (HAT) or deacetylase (HDAC) complexes to the H3K4Me3 mark active transcription. ING5 targets Moz/Morf and HBO1 HAT alter acetylation H3 H4 core histones, affecting gene expression. Previous experiments in vitro indicated functions maintain stem cell character normal cancer cells. Here we find CRISPR/Cas9 knockout (KO) mice sub-fertile but show no decrease lifespan ability heal wounds despite...
Peripheral nerve injuries arising from trauma or disease can lead to sensory and motor deficits neuropathic pain. Despite the purported ability of peripheral self-repair, lifelong disability is common. New molecular cellular insights have begun reveal why has limited repair capacity. The primarily comprised axons Schwann cells, supporting glial cells that produce myelin facilitate rapid conduction electrical impulses. are required for successful regeneration; they partially...
Cellular injury and death are ubiquitous features of disease, yet tools to detect them limited insensitive subtle pathological changes. Acridine orange (AO), a nucleic acid dye with unique spectral properties, enables real-time measurement RNA DNA as proxies for cell viability during exposure various noxious stimuli. This tool illuminates signatures modes death, such cells undergoing apoptosis versus necrosis/necroptosis. new approach also shows that cellular decreases necrotic, necroptotic,...
As an extension of the brain, spinal cord has unique properties which could allow us to gain a better understanding CNS pathology. The brain and share same cellular components, yet latter is simpler in cytoarchitecture connectivity. In Alzheimer's research, virtually all focus on pathology, however it been shown that transgenic mouse models accumulate beta amyloid plaques cord, suggesting possesses molecular machinery conditions for plaque formation. Here we report spatial-temporal map load...
Gene therapies and associated technologies are transforming biomedical research enabling novel therapeutic options for patients living with debilitating incurable genetic disorders. The vector system based on recombinant adeno-associated viral vectors (AAVs) has shown great promise in recent clinical trials diseases of multiple organs, such as the liver nervous system. Despite successes toward development bioengineered AAV variants improved transduction primary human tissues cells, that can...
Dendrimers and dendriplexes, highly branched synthetic macromolecules, have gained popularity as new tools for a variety of nanomedicine strategies due to their unique structure properties. We show that fluorescent phosphorus dendrimers are well retained by bone marrow‐derived macrophages exhibit robust spectral shift in its emission response polarization conditions. Fluorescence properties this marker can also assist identifying macrophage presence phenotype status at different time points...
Abstract Early experimental evidence suggests that predegenerated nerve graft enhance axonal growth compared to freshly harvested in gap repair. However, the practicality of surgically pre-conditioning nerves for grafting remains a major obstacle. Herein, we aim investigate feasibility focal lesioning tissue using high-intensity focused ultrasound (HIFU) and compare regenerative effects grafts rat sciatic transection repair model. As proof principle, were exposed HIFU increase targeting...
Objective: To develop a standardized model of stretch−crush sciatic nerve injury in mice, and to compare outcomes crush novel injuries using standard manual gait sensory assays, them both semi-automated as well deep-learning analysis methods. Methods: Initial studies C57/Bl6 mice were used models followed by histologic analysis. In total, 12 eight-week-old 129S6/SvEvTac six-week behavioural study. Behavioral assessments the von Frey monofilament test recorded on DigiGait platform analyzed...
We previously showed that motor nerves are superior to sensory in promoting axon regeneration after spinal root avulsion. It is, however, impractical use as grafts. One potential approach enhancing axonal using is deliver trophic factors the graft. Here, we examined regulation of receptors for brain-derived neurotrophic factor, glial cell line-derived ciliary and pleiotrophin avulsion adult rats. then tested their survival-promoting neuroregenerative effects on motoneurons. The results...
T‐H. Chu, L. Wang, A. Guo, V. W‐K. Chan, C. W‐M. Wong and W. Wu (2012) Neuropathology Applied Neurobiology 38, 681–695 GDNF‐treated acellular nerve graft promotes motoneuron axon regeneration after implantation into cervical root avulsed spinal cord It is well known that glial cell line‐derived neurotrophic factor (GDNF) a potent for motoneurons. We have previously shown it greatly enhanced survival of peripheral following avulsion. Aims: In the current study, we explore whether injection...
Abstract Skin is an easily accessible tissue and a rich source of Schwann cells (SCs). Toward potential clinical application autologous SC therapies, we aim to improve the reliability specificity our protocol obtain SCs from small skin samples. As well, explore functional distinctions between skin‐derived (Sk‐SCs) nerve‐derived (N‐SCs), used single‐cell RNA‐sequencing series in vitro vivo assays. Our results showed that Sk‐SCs expressed typical markers. Single‐cell sequencing Sk‐ N‐SCs...
The depth of connective tissue window in the side a recipient nerve reverse end-to-side transfers (RETS) remains controversial.To test whether disruption influences efficiency donor axonal regeneration context RETS.Sprague-Dawley rats (n = 24) were assigned to 1 3 groups for obturator motor femoral RETS: group 1, without epineurium opening; 2, with only and 3, perineurium opening. Triple retrograde labeling was used assess number neurons that had regenerated into branch. Thy1-GFP 8) also...
Apurinic/apyrimidinic endonuclease 1 (APE1) is an intermediate enzyme in base excision repair which important for removing damaged nucleotides under normal and pathological conditions. Accumulation of bases causes genome instability jeopardizes cell survival. Our study to examine APE1 regulation oxidative stress spinal motor neurones are vulnerable insult.We challenged the neurone-like line NSC-34 with hydrogen peroxide delineated function by applying various inhibitors. We also examined...