A5020002846- Insect and Pesticide Research
- Plant and animal studies
- Insect and Arachnid Ecology and Behavior
- Bee Products Chemical Analysis
- Healthcare and Venom Research
- Forensic Entomology and Diptera Studies
- Insect behavior and control techniques
- Insect Pest Control Strategies
- Venomous Animal Envenomation and Studies
- Animal Ecology and Behavior Studies
Burapha University
2013-2025
Nosema ceranae causes a widespread disease that reduces honey bee health but is only thought to infect adult bees, not larvae, critical life stage. We reared (Apis mellifera) larvae in vitro and provide the first demonstration N. can decrease subsequent longevity. exposed three-day-old single dose of 40,000 (40K), 10,000 (10K), zero (control), or 40K autoclaved (control) spores larval food. Spores developed intracellularly midgut cells at pre-pupal stage (8 days after egg hatching) 41% bees...
Since Nosema ceranae causes mortality in honey bee populations both managed apiaries and the wild, many chemical compounds have been screened tested for their abilities to suppress of N. ceranae. However, use synthetic substances has resulted residues colonies products. The development new methods control nosema disease is therefore much needed by beekeepers scientists. We evaluated effects propolis from stingless Trigona apicalis on ceranae-infected Asian bees, Apis cerana. One-day-old free...
The health of western honey bees (Apis mellifera L.) is constantly affected by Nosema ceranae, a microsporidian threat to colonies worldwide. We extracted propolis, natural product exhibiting antimicrobial properties, from bee hives, fed it worker before or after infection with N. and determined its ability protect treat them infection. Protection ceranae was tested using newly emerged that were group (50 bees/group) 50% propolis extract for 2, 4 8 d prior 1 × 105 spores per bee. Treatment...
Hypopharyngeal glands of honeybee are age-dependent structures that change with the size acini and correlated various social behaviors. The histochemical structure Apis cerana A. mellifera worker hypopharyngeal in four different developmental stages wes stained ninhydrin Schiff's periodic acid reagents (PAS) for localization proteins carbohydrates, respectively, examined light microscopy. Nurse bees both species had significantly larger as compared to guards forgers, but there were no...
SummaryIn this study, we investigated the bee flora utilised by Thai honey bees, Apis cerana, A. dorsata and florea in Nan province, northern Thailand, through identification of pollen grains from their loads midguts. We compared morphologically to match with local flowering plants determined protein concentration using a Bradford assay. The results showed that 8 families 15 species were found load analysis whilst 12 25 greatest number was (11 species), while there fewer for (10 species)...
Currently, there is widespread concern about declining honey bee populations affected by Nosema ceranae and apis, because infection these pathogens may result in a reduction of pollination services therefore raise concerns shortages. We investigated spp. spore contamination Apis mellifera pollen samples from 34 apiaries three different regions Thailand. spores were detected using light microscopy, species identification molecular characterization the 16S rRNA gene. The numbers spores/gram...
is a large contributing factor to the most recent decline in honey bee health worldwide. Developing new alternative treatments against
There are multiple feedback mechanisms involved in appetite regulation, which is an integral part of maintaining energetic homeostasis. Older forager honey bees, comparison to newly emerged bees and nurse known have highly fluctuating hemolymph trehalose levels, higher changes due starvation, octopamine levels the brain. What remains unknown if interact with one another how this varies as bee ages. We manipulated across age using physiological injections found that increase their increased...
Nosema ceranae is a unicellular microsporidian causing ventricular cell lysis in host Apis species. We hypothesized that the gut microbiota of honey bees, A. cerana, dorsata, florea, and mellifera, disrupted by N. infection. Correspondingly, we investigated impact infection on populations Bifidobacterium sp. Apilactobacillus kunkeei selected The bacteria were compared using culture-dependent method employing 16S rRNA gene sequencing between bees infected with 2.0 × 105N. spores those...
SummaryThe structure of the hypopharyngeal glands Apis andreniformis and florea workers was studied. Glands from pupae, nurse bees foraging were investigated using paraffin staining techniques. Periodic Acid Schiff's reagent (PAS) Ninhydrin Schiffs used for histochemical study carbohydrates proteins respectively. The two bee species found to be quite similar in terms structures, but differences between three life stages. Pupae showed an incomplete glandular which irregular shape. Each...
SummaryIn this study, we tested the responses (attractiveness or repellence) of two species dwarf honey bee (Apis andreniformis and Apis florea) foragers to synthetic mandibular gland pheromones 1-eicosanol, eicosane, heneicosane 2-heptanol. The pheromone concentrations were varied from 0.0, 0.1, 0.5, 1.0, 5.0 10.0% (v/v) in n-hexane. A. repelled by 5.0% eicosane heptanol, while 0.5 1.0% 2-heptanol florea foragers. attracted 0.1% None attractive