A5079546664- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Galectins and Cancer Biology
- RNA and protein synthesis mechanisms
- Extracellular vesicles in disease
- Complement system in diseases
- Protein purification and stability
- RNA Research and Splicing
- Mosquito-borne diseases and control
- RNA regulation and disease
- Analytical Chemistry and Chromatography
Max Planck Institute of Colloids and Interfaces
2016-2023
Freie Universität Berlin
2019-2023
Glycopeptide enrichment is a crucial step in glycoproteomics for which hydrophilic interaction chromatography (HILIC) has extensively been applied due to its low bias towards different glycan types. A systematic evaluation of applicable HILIC mobile phases on glycopeptide efficiency and selectivity is, date, however, still lacking. Here, we present novel, simplified technique termed “Drop-HILIC”, was systematically evaluate the phase effect ZIC-HILIC (zwitterionic type chromatography)...
Abstract The mammalian Staufen proteins (Stau1 and Stau2) mediate degradation of mRNA containing complex secondary structures in their 3’-untranslated region (UTR) through a pathway known as Staufen-mediated decay (SMD). This also involves the RNA helicase UPF1, which is best for its role nonsense-mediated (NMD) pathway. Here we present biochemical reconstitution recruitment activation UPF1 context SMD We demonstrate involvement UPF2, core NMD factor activator SMD. UPF2 acts an adaptor...
Abstract Glycoconjugates on extracellular vesicles (EVs) play a vital role in internalization and mediate interaction as well regulation of the host immune system by viruses, bacteria, parasites. During their intraerythrocytic life‐cycle stages, malaria parasites, Plasmodium falciparum ( Pf ) secretion EVs infected red blood cells (RBCs) that carry diverse range parasitic host‐derived molecules. These molecules facilitate parasite‐parasite parasite‐host interactions to ensure parasite...